Experiment: dodecane 5 vol%
Only strains with sufficient reads to estimate fitness are shown,
but the strain fitness values are still rather noisy and may be biased towards zero.
Strains near the edge of a gene are not shown as being associated with that
gene (they are in grey).
Strains in the central 10-90% of a gene are color coded by the insertion's strand.
Usually, "+" means that the selectable marker is encoded on the forward strand,
i.e., transcribed rightward.
500 nt gutM and srlR are separated by 66 nucleotides srlR and gutQ overlap by 8 nucleotides gutQ and ygaA overlap by 4 nucleotides
b2706: gutM - DNA-binding transcriptional activator of glucitol operon (NCBI), at 2,826,643 to 2,827,002
gutM
b2707: srlR - DNA-bindng transcriptional repressor (NCBI), at 2,827,069 to 2,827,842
srlR
b2708: gutQ - orf, hypothetical protein (VIMSS), at 2,827,835 to 2,828,800
gutQ
b2709: ygaA - putative 2-component transcriptional regulator (VIMSS), at 2,828,797 to 2,830,311
ygaA
Position (kb)
2827
2828
2829 Strain fitness (log2 ratio)
-1
0
1 at 2826.876 kb on - strand, within gutM at 2826.932 kb on + strand, within gutM at 2826.963 kb on - strand, within gutM at 2827.166 kb on - strand, within srlR at 2827.166 kb on - strand, within srlR at 2827.191 kb on - strand, within srlR at 2827.191 kb on - strand, within srlR at 2827.193 kb on + strand, within srlR at 2827.193 kb on + strand, within srlR at 2827.201 kb on - strand, within srlR at 2827.298 kb on + strand, within srlR at 2827.368 kb on + strand, within srlR at 2827.425 kb on + strand, within srlR at 2827.431 kb on + strand, within srlR at 2827.481 kb on + strand, within srlR at 2827.483 kb on - strand, within srlR at 2827.549 kb on + strand, within srlR at 2827.619 kb on + strand, within srlR at 2827.619 kb on + strand, within srlR at 2827.893 kb on - strand at 2828.144 kb on + strand, within gutQ at 2828.242 kb on - strand, within gutQ at 2828.313 kb on - strand, within gutQ at 2828.314 kb on - strand, within gutQ at 2828.394 kb on - strand, within gutQ at 2828.394 kb on - strand, within gutQ at 2828.394 kb on - strand, within gutQ at 2828.405 kb on - strand, within gutQ at 2829.156 kb on - strand, within ygaA at 2829.156 kb on - strand, within ygaA at 2829.382 kb on + strand, within ygaA at 2829.382 kb on + strand, within ygaA at 2829.400 kb on + strand, within ygaA at 2829.417 kb on + strand, within ygaA at 2829.527 kb on + strand, within ygaA at 2829.527 kb on + strand, within ygaA at 2829.544 kb on - strand, within ygaA at 2829.583 kb on + strand, within ygaA at 2829.583 kb on + strand, within ygaA at 2829.687 kb on + strand, within ygaA at 2829.687 kb on + strand, within ygaA
Per-strain Table
Position Strand Gene LocusTag Fraction dodecane 5 vol% remove 2,826,876 - gutM b2706 0.65 +0.4 2,826,932 + gutM b2706 0.80 +0.1 2,826,963 - gutM b2706 0.89 -0.3 2,827,166 - srlR b2707 0.13 +0.1 2,827,166 - srlR b2707 0.13 +0.2 2,827,191 - srlR b2707 0.16 -0.9 2,827,191 - srlR b2707 0.16 -0.9 2,827,193 + srlR b2707 0.16 +0.1 2,827,193 + srlR b2707 0.16 +0.4 2,827,201 - srlR b2707 0.17 -0.1 2,827,298 + srlR b2707 0.30 -0.1 2,827,368 + srlR b2707 0.39 +0.5 2,827,425 + srlR b2707 0.46 +0.1 2,827,431 + srlR b2707 0.47 +0.2 2,827,481 + srlR b2707 0.53 +1.1 2,827,483 - srlR b2707 0.53 +0.4 2,827,549 + srlR b2707 0.62 -1.3 2,827,619 + srlR b2707 0.71 +0.4 2,827,619 + srlR b2707 0.71 -0.6 2,827,893 - +0.2 2,828,144 + gutQ b2708 0.32 +0.5 2,828,242 - gutQ b2708 0.42 -1.6 2,828,313 - gutQ b2708 0.49 +0.2 2,828,314 - gutQ b2708 0.50 -0.1 2,828,394 - gutQ b2708 0.58 -0.7 2,828,394 - gutQ b2708 0.58 +0.9 2,828,394 - gutQ b2708 0.58 +0.2 2,828,405 - gutQ b2708 0.59 -0.3 2,829,156 - ygaA b2709 0.24 -0.1 2,829,156 - ygaA b2709 0.24 +0.1 2,829,382 + ygaA b2709 0.39 +0.4 2,829,382 + ygaA b2709 0.39 +0.5 2,829,400 + ygaA b2709 0.40 -0.0 2,829,417 + ygaA b2709 0.41 +0.3 2,829,527 + ygaA b2709 0.48 -0.2 2,829,527 + ygaA b2709 0.48 +0.0 2,829,544 - ygaA b2709 0.49 +0.6 2,829,583 + ygaA b2709 0.52 +0.1 2,829,583 + ygaA b2709 0.52 +0.3 2,829,687 + ygaA b2709 0.59 +0.3 2,829,687 + ygaA b2709 0.59 +0.3
Or see this region's nucleotide sequence