Experiment: LB_plus_SM_buffer with EIP16_phage 0.0875 MOI
Only strains with sufficient reads to estimate fitness are shown,
but the strain fitness values are still rather noisy and may be biased towards zero.
Strains near the edge of a gene are not shown as being associated with that
gene (they are in grey).
Strains in the central 10-90% of a gene are color coded by the insertion's strand.
Usually, "+" means that the selectable marker is encoded on the forward strand,
i.e., transcribed rightward.
500 nt GFF916 and GFF917 overlap by 11 nucleotides GFF917 and GFF918 are separated by 3 nucleotides GFF918 and GFF919 are separated by 120 nucleotides GFF919 and GFF920 are separated by 204 nucleotides GFF920 and GFF921 are separated by 33 nucleotides
GFF916 - RelB/StbD replicon stabilization protein (antitoxin to RelE/StbE), at 94,800 to 95,042
GFF916
GFF917 - RelE/StbE replicon stabilization toxin, at 95,032 to 95,316
GFF917
GFF918 - Ribonucleotide reductase of class III (anaerobic), activating protein (EC 1.97.1.4), at 95,320 to 95,784
GFF918
GFF919 - Ribonucleotide reductase of class III (anaerobic), large subunit (EC 1.17.4.2), at 95,905 to 98,043
GFF919
GFF920 - FIG01045722: hypothetical protein, at 98,248 to 98,418
GFF920
GFF921 - Trehalose-6-phosphate hydrolase (EC 3.2.1.93), at 98,452 to 100,104
GFF921
Position (kb) 95
96
97
98
99 Strain fitness (log2 ratio) -1
0
1
2 at 95.038 kb on + strand at 95.038 kb on + strand at 95.038 kb on + strand at 95.038 kb on + strand at 95.079 kb on + strand, within GFF917 at 95.080 kb on - strand, within GFF917 at 95.132 kb on + strand, within GFF917 at 95.132 kb on + strand, within GFF917 at 95.196 kb on + strand, within GFF917 at 95.315 kb on - strand at 95.485 kb on - strand, within GFF918 at 95.519 kb on - strand, within GFF918 at 95.519 kb on - strand, within GFF918 at 95.625 kb on + strand, within GFF918 at 95.626 kb on - strand, within GFF918 at 95.643 kb on + strand, within GFF918 at 95.684 kb on + strand, within GFF918 at 95.765 kb on + strand at 95.806 kb on + strand at 95.806 kb on + strand at 95.815 kb on + strand at 95.911 kb on + strand at 95.912 kb on - strand at 95.927 kb on - strand at 95.927 kb on - strand at 96.111 kb on - strand at 96.143 kb on + strand, within GFF919 at 96.148 kb on + strand, within GFF919 at 96.149 kb on - strand, within GFF919 at 96.164 kb on + strand, within GFF919 at 96.254 kb on + strand, within GFF919 at 96.254 kb on + strand, within GFF919 at 96.281 kb on + strand, within GFF919 at 96.329 kb on + strand, within GFF919 at 96.419 kb on + strand, within GFF919 at 96.660 kb on + strand, within GFF919 at 96.663 kb on - strand, within GFF919 at 96.816 kb on + strand, within GFF919 at 96.816 kb on + strand, within GFF919 at 96.941 kb on + strand, within GFF919 at 96.997 kb on - strand, within GFF919 at 97.117 kb on - strand, within GFF919 at 97.171 kb on - strand, within GFF919 at 97.242 kb on - strand, within GFF919 at 97.390 kb on - strand, within GFF919 at 97.410 kb on + strand, within GFF919 at 97.479 kb on + strand, within GFF919 at 97.518 kb on + strand, within GFF919 at 97.518 kb on + strand, within GFF919 at 97.519 kb on - strand, within GFF919 at 97.519 kb on - strand, within GFF919 at 97.547 kb on + strand, within GFF919 at 97.562 kb on + strand, within GFF919 at 97.591 kb on + strand, within GFF919 at 97.594 kb on + strand, within GFF919 at 97.594 kb on + strand, within GFF919 at 97.625 kb on + strand, within GFF919 at 97.678 kb on - strand, within GFF919 at 97.793 kb on + strand, within GFF919 at 97.932 kb on - strand at 97.969 kb on + strand at 97.983 kb on + strand at 98.242 kb on + strand at 98.439 kb on + strand at 98.439 kb on + strand at 98.439 kb on + strand at 98.439 kb on + strand at 98.439 kb on + strand at 98.440 kb on - strand at 98.453 kb on + strand at 98.461 kb on + strand at 98.462 kb on - strand at 98.526 kb on - strand at 98.530 kb on - strand at 98.560 kb on - strand at 98.560 kb on - strand at 98.648 kb on - strand, within GFF921 at 98.733 kb on - strand, within GFF921 at 98.738 kb on + strand, within GFF921 at 98.739 kb on - strand, within GFF921 at 98.790 kb on - strand, within GFF921 at 99.030 kb on + strand, within GFF921 at 99.030 kb on + strand, within GFF921 at 99.031 kb on - strand, within GFF921 at 99.031 kb on - strand, within GFF921 at 99.031 kb on - strand, within GFF921 at 99.031 kb on - strand, within GFF921 at 99.033 kb on - strand, within GFF921
Per-strain Table
Position Strand Gene LocusTag Fraction LB_plus_SM_buffer with EIP16_phage 0.0875 MOI remove 95,038 + -0.1 95,038 + -0.3 95,038 + +0.1 95,038 + -0.6 95,079 + GFF917 0.16 -0.0 95,080 - GFF917 0.17 +0.5 95,132 + GFF917 0.35 -0.1 95,132 + GFF917 0.35 +0.7 95,196 + GFF917 0.58 +0.3 95,315 - -0.2 95,485 - GFF918 0.35 +1.5 95,519 - GFF918 0.43 +0.3 95,519 - GFF918 0.43 -0.2 95,625 + GFF918 0.66 -0.7 95,626 - GFF918 0.66 -0.0 95,643 + GFF918 0.69 -0.8 95,684 + GFF918 0.78 +0.4 95,765 + +0.3 95,806 + -0.4 95,806 + -0.9 95,815 + -0.4 95,911 + -0.4 95,912 - +0.1 95,927 - +0.1 95,927 - -0.5 96,111 - +0.6 96,143 + GFF919 0.11 +0.1 96,148 + GFF919 0.11 -0.5 96,149 - GFF919 0.11 +1.2 96,164 + GFF919 0.12 -0.3 96,254 + GFF919 0.16 +1.7 96,254 + GFF919 0.16 +0.0 96,281 + GFF919 0.18 +1.7 96,329 + GFF919 0.20 -0.0 96,419 + GFF919 0.24 -0.1 96,660 + GFF919 0.35 +0.0 96,663 - GFF919 0.35 +0.4 96,816 + GFF919 0.43 -0.4 96,816 + GFF919 0.43 +0.2 96,941 + GFF919 0.48 -0.9 96,997 - GFF919 0.51 -0.9 97,117 - GFF919 0.57 -0.3 97,171 - GFF919 0.59 +0.1 97,242 - GFF919 0.63 -0.3 97,390 - GFF919 0.69 -0.3 97,410 + GFF919 0.70 -0.5 97,479 + GFF919 0.74 -0.1 97,518 + GFF919 0.75 -1.0 97,518 + GFF919 0.75 +0.5 97,519 - GFF919 0.75 -0.4 97,519 - GFF919 0.75 +0.5 97,547 + GFF919 0.77 -1.1 97,562 + GFF919 0.77 -0.1 97,591 + GFF919 0.79 +0.2 97,594 + GFF919 0.79 +0.8 97,594 + GFF919 0.79 -0.0 97,625 + GFF919 0.80 +0.1 97,678 - GFF919 0.83 -0.3 97,793 + GFF919 0.88 +1.8 97,932 - -0.2 97,969 + -0.0 97,983 + +0.2 98,242 + -0.1 98,439 + +0.4 98,439 + +0.3 98,439 + +0.0 98,439 + -0.2 98,439 + +0.6 98,440 - -0.5 98,453 + +0.3 98,461 + +0.4 98,462 - +0.6 98,526 - +0.5 98,530 - -0.3 98,560 - -0.2 98,560 - -0.3 98,648 - GFF921 0.12 +0.0 98,733 - GFF921 0.17 -0.1 98,738 + GFF921 0.17 +0.8 98,739 - GFF921 0.17 +0.3 98,790 - GFF921 0.20 +0.0 99,030 + GFF921 0.35 -0.8 99,030 + GFF921 0.35 +0.1 99,031 - GFF921 0.35 +0.0 99,031 - GFF921 0.35 +0.0 99,031 - GFF921 0.35 +1.2 99,031 - GFF921 0.35 +0.4 99,033 - GFF921 0.35 +0.8
Or see this region's nucleotide sequence