Experiment: LB_plus_SM_buffer with EIP16_phage 0.0875 MOI
Only strains with sufficient reads to estimate fitness are shown,
but the strain fitness values are still rather noisy and may be biased towards zero.
Strains near the edge of a gene are not shown as being associated with that
gene (they are in grey).
Strains in the central 10-90% of a gene are color coded by the insertion's strand.
Usually, "+" means that the selectable marker is encoded on the forward strand,
i.e., transcribed rightward.
500 nt GFF902 and GFF903 are separated by 55 nucleotides GFF903 and GFF904 are separated by 107 nucleotides GFF904 and GFF905 are separated by 98 nucleotides GFF905 and GFF906 are separated by 3 nucleotides GFF906 and GFF907 are separated by 160 nucleotides
GFF902 - Glyceraldehyde-3-phosphate ketol-isomerase (EC 5.3.1.1), at 83,591 to 84,475
GFF902
GFF903 - FIG138315: Putative alpha helix protein, at 84,531 to 85,082
GFF903
GFF904 - TldE protein, part of TldE/TldD proteolytic complex, at 85,190 to 86,530
GFF904
GFF905 - Soluble cytochrome b562, at 86,629 to 87,015
GFF905
GFF906 - FIG01047835: hypothetical protein, at 87,019 to 87,141
GFF906
GFF907 - FIG01045571: hypothetical protein, at 87,302 to 87,631
GFF907
Position (kb)
85
86
87 Strain fitness (log2 ratio)
-2
-1
0
1
2 at 84.238 kb on + strand, within GFF902 at 85.172 kb on + strand at 85.172 kb on + strand at 85.173 kb on - strand at 85.342 kb on - strand, within GFF904 at 85.381 kb on + strand, within GFF904 at 85.381 kb on + strand, within GFF904 at 85.389 kb on - strand, within GFF904 at 85.685 kb on - strand, within GFF904 at 85.797 kb on - strand, within GFF904 at 85.797 kb on - strand, within GFF904 at 85.945 kb on - strand, within GFF904 at 85.986 kb on - strand, within GFF904 at 85.990 kb on + strand, within GFF904 at 85.990 kb on + strand, within GFF904 at 86.171 kb on + strand, within GFF904 at 86.171 kb on + strand, within GFF904 at 86.339 kb on + strand, within GFF904 at 86.340 kb on - strand, within GFF904 at 86.405 kb on - strand at 86.440 kb on + strand at 86.479 kb on + strand at 86.481 kb on + strand at 86.481 kb on + strand at 86.599 kb on + strand at 86.645 kb on + strand at 86.709 kb on + strand, within GFF905 at 86.713 kb on - strand, within GFF905 at 86.991 kb on + strand at 86.991 kb on + strand at 86.992 kb on - strand at 87.000 kb on + strand at 87.001 kb on - strand at 87.001 kb on - strand at 87.001 kb on - strand at 87.007 kb on + strand at 87.070 kb on - strand, within GFF906 at 87.202 kb on - strand at 87.273 kb on - strand at 87.366 kb on + strand, within GFF907 at 87.469 kb on + strand, within GFF907
Per-strain Table
Position Strand Gene LocusTag Fraction LB_plus_SM_buffer with EIP16_phage 0.0875 MOI remove 84,238 + GFF902 0.73 -0.7 85,172 + +1.8 85,172 + -0.6 85,173 - -0.1 85,342 - GFF904 0.11 -0.4 85,381 + GFF904 0.14 -0.3 85,381 + GFF904 0.14 +0.4 85,389 - GFF904 0.15 -0.4 85,685 - GFF904 0.37 -0.1 85,797 - GFF904 0.45 -0.2 85,797 - GFF904 0.45 -0.7 85,945 - GFF904 0.56 +0.5 85,986 - GFF904 0.59 -0.0 85,990 + GFF904 0.60 -0.7 85,990 + GFF904 0.60 -0.7 86,171 + GFF904 0.73 -0.2 86,171 + GFF904 0.73 -0.2 86,339 + GFF904 0.86 +0.5 86,340 - GFF904 0.86 +0.1 86,405 - +0.2 86,440 + -1.9 86,479 + -1.4 86,481 + -0.5 86,481 + +0.2 86,599 + -0.2 86,645 + -0.3 86,709 + GFF905 0.21 +0.1 86,713 - GFF905 0.22 +0.1 86,991 + -0.2 86,991 + +0.1 86,992 - -0.4 87,000 + -0.1 87,001 - -0.2 87,001 - -0.3 87,001 - +0.9 87,007 + +0.5 87,070 - GFF906 0.41 -0.1 87,202 - -0.0 87,273 - +0.0 87,366 + GFF907 0.19 -0.0 87,469 + GFF907 0.51 +0.9
Or see this region's nucleotide sequence