Experiment: LB_plus_SM_buffer with EIP16_phage 0.0875 MOI
Only strains with sufficient reads to estimate fitness are shown,
but the strain fitness values are still rather noisy and may be biased towards zero.
Strains near the edge of a gene are not shown as being associated with that
gene (they are in grey).
Strains in the central 10-90% of a gene are color coded by the insertion's strand.
Usually, "+" means that the selectable marker is encoded on the forward strand,
i.e., transcribed rightward.
500 nt GFF618 and GFF619 are separated by 18 nucleotides GFF619 and GFF620 are separated by 10 nucleotides GFF620 and GFF621 are separated by 64 nucleotides
GFF618 - 33 kDa chaperonin (Heat shock protein 33) (HSP33), at 358,715 to 359,599
GFF618
GFF619 - Ribosome-associated heat shock protein implicated in the recycling of the 50S subunit (S4 paralog), at 359,618 to 360,019
GFF619
GFF620 - FIG001957: putative hydrolase, at 360,030 to 360,698
GFF620
GFF621 - IgaA: a membrane protein that prevents overactivation of the Rcs regulatory system, at 360,763 to 362,895
GFF621
Position (kb) 360
361 Strain fitness (log2 ratio) -1
0
1
2 at 359.191 kb on + strand, within GFF618 at 359.220 kb on + strand, within GFF618 at 359.243 kb on - strand, within GFF618 at 359.433 kb on - strand, within GFF618 at 359.574 kb on - strand at 359.574 kb on - strand at 359.594 kb on - strand at 359.620 kb on - strand at 359.707 kb on + strand, within GFF619 at 359.890 kb on + strand, within GFF619 at 359.890 kb on + strand, within GFF619 at 359.890 kb on + strand, within GFF619 at 359.907 kb on + strand, within GFF619 at 359.907 kb on + strand, within GFF619 at 359.907 kb on + strand, within GFF619 at 359.907 kb on + strand, within GFF619 at 359.908 kb on - strand, within GFF619 at 360.083 kb on + strand at 360.083 kb on + strand at 360.083 kb on + strand at 360.207 kb on + strand, within GFF620 at 360.283 kb on - strand, within GFF620 at 360.286 kb on - strand, within GFF620 at 360.335 kb on + strand, within GFF620 at 360.385 kb on - strand, within GFF620 at 360.402 kb on + strand, within GFF620 at 360.407 kb on + strand, within GFF620 at 360.496 kb on + strand, within GFF620 at 360.509 kb on + strand, within GFF620 at 360.510 kb on - strand, within GFF620 at 360.516 kb on + strand, within GFF620 at 360.516 kb on + strand, within GFF620 at 360.517 kb on - strand, within GFF620 at 360.526 kb on + strand, within GFF620 at 360.539 kb on + strand, within GFF620 at 360.540 kb on - strand, within GFF620 at 360.541 kb on + strand, within GFF620 at 360.541 kb on + strand, within GFF620 at 360.613 kb on + strand, within GFF620 at 360.613 kb on + strand, within GFF620 at 360.692 kb on + strand at 360.692 kb on + strand
Per-strain Table
Position Strand Gene LocusTag Fraction LB_plus_SM_buffer with EIP16_phage 0.0875 MOI remove 359,191 + GFF618 0.54 +1.8 359,220 + GFF618 0.57 +1.3 359,243 - GFF618 0.60 +1.2 359,433 - GFF618 0.81 +1.0 359,574 - +1.2 359,574 - +1.1 359,594 - +1.6 359,620 - +1.3 359,707 + GFF619 0.22 +0.4 359,890 + GFF619 0.68 +2.2 359,890 + GFF619 0.68 +0.6 359,890 + GFF619 0.68 +1.2 359,907 + GFF619 0.72 +1.5 359,907 + GFF619 0.72 +1.1 359,907 + GFF619 0.72 +0.9 359,907 + GFF619 0.72 +1.4 359,908 - GFF619 0.72 -0.1 360,083 + +1.0 360,083 + +1.2 360,083 + +1.2 360,207 + GFF620 0.26 +1.2 360,283 - GFF620 0.38 +0.3 360,286 - GFF620 0.38 +1.3 360,335 + GFF620 0.46 -0.1 360,385 - GFF620 0.53 -0.1 360,402 + GFF620 0.56 -0.1 360,407 + GFF620 0.56 +0.8 360,496 + GFF620 0.70 -0.0 360,509 + GFF620 0.72 +2.0 360,510 - GFF620 0.72 +0.5 360,516 + GFF620 0.73 -0.0 360,516 + GFF620 0.73 -0.2 360,517 - GFF620 0.73 -0.1 360,526 + GFF620 0.74 +0.2 360,539 + GFF620 0.76 +0.1 360,540 - GFF620 0.76 +0.4 360,541 + GFF620 0.76 -0.2 360,541 + GFF620 0.76 +1.3 360,613 + GFF620 0.87 +0.2 360,613 + GFF620 0.87 +0.3 360,692 + +0.5 360,692 + -0.9
Or see this region's nucleotide sequence