Strain Fitness in Salmonella enterica subsp. enterica serovar Typhimurium str. MS1868 around GFF615

Experiment: LB_plus_SM_buffer with EIP16_phage 0.0875 MOI

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Only strains with sufficient reads to estimate fitness are shown, but the strain fitness values are still rather noisy and may be biased towards zero. Strains near the edge of a gene are not shown as being associated with that gene (they are in grey). Strains in the central 10-90% of a gene are color coded by the insertion's strand. Usually, "+" means that the selectable marker is encoded on the forward strand, i.e., transcribed rightward.

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500 ntGFF614 and GFF615 are separated by 74 nucleotidesGFF615 and GFF616 are separated by 53 nucleotidesGFF616 and GFF617 are separated by 191 nucleotides GFF614 - Osmolarity sensory histidine kinase EnvZ, at 353,479 to 354,822 GFF614 GFF615 - Phosphoenolpyruvate carboxykinase [ATP] (EC 4.1.1.49), at 354,897 to 356,516 GFF615 GFF616 - hypothetical protein, at 356,570 to 356,704 GFF616 GFF617 - Putative transport protein, at 356,896 to 358,605 GFF617 Position (kb) 354 355 356 357Strain fitness (log2 ratio) -1 0 1 2at 354.012 kb on - strand, within GFF614at 354.056 kb on + strand, within GFF614at 354.070 kb on - strand, within GFF614at 354.135 kb on + strand, within GFF614at 354.321 kb on + strand, within GFF614at 354.322 kb on - strand, within GFF614at 354.426 kb on + strand, within GFF614at 354.499 kb on - strand, within GFF614at 354.660 kb on + strand, within GFF614at 354.820 kb on + strandat 354.820 kb on + strandat 354.821 kb on - strandat 354.833 kb on + strandat 354.833 kb on + strandat 354.833 kb on + strandat 354.833 kb on + strandat 354.836 kb on + strandat 354.836 kb on + strandat 354.947 kb on + strandat 355.021 kb on + strandat 355.157 kb on + strand, within GFF615at 355.172 kb on + strand, within GFF615at 355.173 kb on - strand, within GFF615at 355.211 kb on - strand, within GFF615at 355.235 kb on + strand, within GFF615at 355.236 kb on - strand, within GFF615at 355.258 kb on + strand, within GFF615at 355.258 kb on + strand, within GFF615at 355.259 kb on - strand, within GFF615at 355.352 kb on + strand, within GFF615at 355.504 kb on + strand, within GFF615at 355.504 kb on + strand, within GFF615at 355.505 kb on - strand, within GFF615at 355.839 kb on - strand, within GFF615at 355.858 kb on + strand, within GFF615at 355.925 kb on + strand, within GFF615at 356.018 kb on + strand, within GFF615at 356.240 kb on + strand, within GFF615at 356.279 kb on + strand, within GFF615at 356.306 kb on + strand, within GFF615at 356.306 kb on + strand, within GFF615at 356.306 kb on + strand, within GFF615at 356.307 kb on - strand, within GFF615at 356.308 kb on + strand, within GFF615at 356.308 kb on + strand, within GFF615at 356.308 kb on + strand, within GFF615at 356.308 kb on + strand, within GFF615at 356.309 kb on - strand, within GFF615at 356.309 kb on - strand, within GFF615at 356.309 kb on - strand, within GFF615at 356.309 kb on - strand, within GFF615at 356.312 kb on + strand, within GFF615at 356.370 kb on - strandat 356.373 kb on + strandat 356.373 kb on + strandat 356.390 kb on - strandat 356.408 kb on + strandat 356.423 kb on - strandat 356.434 kb on + strandat 356.476 kb on + strandat 356.477 kb on - strandat 356.538 kb on + strandat 356.539 kb on - strandat 356.539 kb on - strandat 356.539 kb on - strandat 356.540 kb on + strandat 356.593 kb on + strand, within GFF616at 356.600 kb on - strand, within GFF616at 356.736 kb on + strandat 356.737 kb on - strandat 356.761 kb on + strandat 356.919 kb on + strandat 356.920 kb on - strandat 356.922 kb on + strandat 356.922 kb on + strandat 356.971 kb on + strandat 356.972 kb on - strandat 356.972 kb on - strandat 357.003 kb on + strandat 357.020 kb on - strandat 357.020 kb on - strandat 357.020 kb on - strandat 357.024 kb on + strandat 357.057 kb on - strandat 357.057 kb on - strandat 357.064 kb on - strandat 357.078 kb on + strand, within GFF617at 357.202 kb on + strand, within GFF617at 357.253 kb on + strand, within GFF617at 357.320 kb on + strand, within GFF617at 357.320 kb on + strand, within GFF617at 357.448 kb on - strand, within GFF617at 357.452 kb on - strand, within GFF617

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Per-strain Table

Position Strand Gene LocusTag Fraction LB_plus_SM_buffer with EIP16_phage 0.0875 MOI
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354,012 - GFF614 0.40 +1.3
354,056 + GFF614 0.43 +1.7
354,070 - GFF614 0.44 +1.4
354,135 + GFF614 0.49 +1.5
354,321 + GFF614 0.63 +1.8
354,322 - GFF614 0.63 +2.1
354,426 + GFF614 0.70 +1.3
354,499 - GFF614 0.76 +1.6
354,660 + GFF614 0.88 +1.6
354,820 + +0.2
354,820 + +0.0
354,821 - +1.8
354,833 + +0.9
354,833 + +0.4
354,833 + +0.3
354,833 + +0.0
354,836 + +1.3
354,836 + +1.4
354,947 + -0.0
355,021 + +0.4
355,157 + GFF615 0.16 +1.4
355,172 + GFF615 0.17 +0.1
355,173 - GFF615 0.17 -0.7
355,211 - GFF615 0.19 -0.1
355,235 + GFF615 0.21 -0.2
355,236 - GFF615 0.21 -1.1
355,258 + GFF615 0.22 -0.3
355,258 + GFF615 0.22 +0.3
355,259 - GFF615 0.22 -0.1
355,352 + GFF615 0.28 -0.0
355,504 + GFF615 0.37 +0.7
355,504 + GFF615 0.37 -0.3
355,505 - GFF615 0.38 +1.0
355,839 - GFF615 0.58 +1.7
355,858 + GFF615 0.59 -0.4
355,925 + GFF615 0.63 -0.1
356,018 + GFF615 0.69 -0.4
356,240 + GFF615 0.83 +0.0
356,279 + GFF615 0.85 +0.1
356,306 + GFF615 0.87 +0.9
356,306 + GFF615 0.87 -0.1
356,306 + GFF615 0.87 -0.1
356,307 - GFF615 0.87 -0.5
356,308 + GFF615 0.87 +0.1
356,308 + GFF615 0.87 -0.2
356,308 + GFF615 0.87 -0.1
356,308 + GFF615 0.87 +0.2
356,309 - GFF615 0.87 +0.2
356,309 - GFF615 0.87 -0.1
356,309 - GFF615 0.87 -0.5
356,309 - GFF615 0.87 -0.0
356,312 + GFF615 0.87 +1.1
356,370 - -0.2
356,373 + -0.1
356,373 + +0.1
356,390 - -0.4
356,408 + +0.0
356,423 - -0.1
356,434 + +0.2
356,476 + -0.7
356,477 - +0.5
356,538 + -0.5
356,539 - -0.8
356,539 - -0.2
356,539 - -0.2
356,540 + -0.4
356,593 + GFF616 0.17 -0.5
356,600 - GFF616 0.22 +0.3
356,736 + +0.2
356,737 - -0.3
356,761 + +0.0
356,919 + +0.2
356,920 - -0.6
356,922 + +0.2
356,922 + -1.3
356,971 + +1.4
356,972 - +0.3
356,972 - +0.4
357,003 + +0.4
357,020 - -0.0
357,020 - +0.4
357,020 - -0.1
357,024 + +0.3
357,057 - +0.0
357,057 - +2.0
357,064 - +0.4
357,078 + GFF617 0.11 -0.1
357,202 + GFF617 0.18 -0.1
357,253 + GFF617 0.21 +0.2
357,320 + GFF617 0.25 +0.0
357,320 + GFF617 0.25 +0.5
357,448 - GFF617 0.32 +0.3
357,452 - GFF617 0.33 +0.4

Or see this region's nucleotide sequence