Experiment: LB_plus_SM_buffer with EIP16_phage 0.0875 MOI
Only strains with sufficient reads to estimate fitness are shown,
but the strain fitness values are still rather noisy and may be biased towards zero.
Strains near the edge of a gene are not shown as being associated with that
gene (they are in grey).
Strains in the central 10-90% of a gene are color coded by the insertion's strand.
Usually, "+" means that the selectable marker is encoded on the forward strand,
i.e., transcribed rightward.
500 nt GFF606 and GFF607 are separated by 69 nucleotides GFF607 and GFF608 are separated by 51 nucleotides GFF608 and GFF609 are separated by 12 nucleotides GFF609 and GFF610 are separated by 18 nucleotides GFF610 and GFF611 are separated by 436 nucleotides
GFF606 - Uncharacterized protein YfaD, at 345,227 to 346,141
GFF606
GFF607 - hypothetical protein, at 346,211 to 346,324
GFF607
GFF608 - Ferrous iron-sensisng transcriptional regulator FeoC, at 346,376 to 346,612
GFF608
GFF609 - Ferrous iron transport protein B, at 346,625 to 348,943
GFF609
GFF610 - Ferrous iron transport protein A, at 348,962 to 349,189
GFF610
GFF611 - Transcription accessory protein (S1 RNA-binding domain), at 349,626 to 351,956
GFF611
Position (kb) 346
347
348
349 Strain fitness (log2 ratio) -5
-4
-3
-2
-1
0
1
2 at 345.694 kb on - strand, within GFF606 at 345.696 kb on - strand, within GFF606 at 345.701 kb on - strand, within GFF606 at 345.808 kb on + strand, within GFF606 at 345.872 kb on - strand, within GFF606 at 345.872 kb on - strand, within GFF606 at 345.886 kb on + strand, within GFF606 at 345.909 kb on + strand, within GFF606 at 345.915 kb on + strand, within GFF606 at 346.240 kb on + strand, within GFF607 at 346.324 kb on + strand at 346.324 kb on + strand at 346.356 kb on - strand at 346.356 kb on - strand at 346.412 kb on - strand, within GFF608 at 346.468 kb on + strand, within GFF608 at 346.541 kb on - strand, within GFF608 at 346.617 kb on + strand at 346.626 kb on + strand at 346.728 kb on - strand at 346.776 kb on + strand at 346.776 kb on + strand at 346.831 kb on - strand at 346.913 kb on - strand, within GFF609 at 346.920 kb on - strand, within GFF609 at 346.923 kb on + strand, within GFF609 at 346.950 kb on + strand, within GFF609 at 347.023 kb on + strand, within GFF609 at 347.023 kb on + strand, within GFF609 at 347.059 kb on + strand, within GFF609 at 347.060 kb on - strand, within GFF609 at 347.317 kb on + strand, within GFF609 at 347.374 kb on + strand, within GFF609 at 347.518 kb on + strand, within GFF609 at 347.519 kb on - strand, within GFF609 at 347.544 kb on - strand, within GFF609 at 347.657 kb on - strand, within GFF609 at 347.735 kb on - strand, within GFF609 at 347.735 kb on - strand, within GFF609 at 347.735 kb on - strand, within GFF609 at 347.827 kb on + strand, within GFF609 at 347.828 kb on - strand, within GFF609 at 347.828 kb on - strand, within GFF609 at 347.862 kb on + strand, within GFF609 at 348.303 kb on + strand, within GFF609 at 348.303 kb on + strand, within GFF609 at 348.350 kb on + strand, within GFF609 at 348.350 kb on + strand, within GFF609 at 348.415 kb on - strand, within GFF609 at 348.598 kb on + strand, within GFF609 at 348.598 kb on + strand, within GFF609 at 348.599 kb on - strand, within GFF609 at 348.608 kb on - strand, within GFF609 at 348.637 kb on - strand, within GFF609 at 348.763 kb on - strand at 348.823 kb on + strand at 348.884 kb on - strand at 348.888 kb on - strand at 348.888 kb on - strand at 348.888 kb on - strand at 348.963 kb on + strand at 348.987 kb on - strand, within GFF610 at 349.006 kb on + strand, within GFF610 at 349.079 kb on - strand, within GFF610 at 349.190 kb on - strand at 349.196 kb on + strand at 349.196 kb on + strand at 349.197 kb on - strand at 349.207 kb on + strand at 349.207 kb on + strand at 349.220 kb on + strand at 349.220 kb on + strand at 349.220 kb on + strand at 349.221 kb on - strand at 349.221 kb on - strand at 349.222 kb on + strand at 349.265 kb on + strand at 349.814 kb on + strand at 349.815 kb on - strand at 349.821 kb on - strand at 349.821 kb on - strand at 349.821 kb on - strand
Per-strain Table
Position Strand Gene LocusTag Fraction LB_plus_SM_buffer with EIP16_phage 0.0875 MOI remove 345,694 - GFF606 0.51 +0.4 345,696 - GFF606 0.51 +0.8 345,701 - GFF606 0.52 +1.7 345,808 + GFF606 0.63 +0.5 345,872 - GFF606 0.70 -0.0 345,872 - GFF606 0.70 +0.3 345,886 + GFF606 0.72 -0.5 345,909 + GFF606 0.75 -0.2 345,915 + GFF606 0.75 +0.2 346,240 + GFF607 0.25 -0.5 346,324 + +0.0 346,324 + -0.6 346,356 - -0.8 346,356 - -0.1 346,412 - GFF608 0.15 -4.9 346,468 + GFF608 0.39 -0.9 346,541 - GFF608 0.70 -2.5 346,617 + -1.9 346,626 + -2.3 346,728 - -3.6 346,776 + -3.0 346,776 + -2.6 346,831 - -1.9 346,913 - GFF609 0.12 -1.8 346,920 - GFF609 0.13 -2.5 346,923 + GFF609 0.13 -3.2 346,950 + GFF609 0.14 -3.0 347,023 + GFF609 0.17 -0.4 347,023 + GFF609 0.17 -0.5 347,059 + GFF609 0.19 -3.4 347,060 - GFF609 0.19 -2.7 347,317 + GFF609 0.30 -3.7 347,374 + GFF609 0.32 -3.5 347,518 + GFF609 0.39 -0.8 347,519 - GFF609 0.39 -2.5 347,544 - GFF609 0.40 -3.0 347,657 - GFF609 0.45 -2.6 347,735 - GFF609 0.48 -0.8 347,735 - GFF609 0.48 -1.0 347,735 - GFF609 0.48 -2.8 347,827 + GFF609 0.52 -2.5 347,828 - GFF609 0.52 -3.1 347,828 - GFF609 0.52 -2.0 347,862 + GFF609 0.53 -4.0 348,303 + GFF609 0.72 -2.1 348,303 + GFF609 0.72 -2.7 348,350 + GFF609 0.74 -1.9 348,350 + GFF609 0.74 -3.3 348,415 - GFF609 0.77 -2.9 348,598 + GFF609 0.85 -2.2 348,598 + GFF609 0.85 -2.5 348,599 - GFF609 0.85 -3.3 348,608 - GFF609 0.86 -2.5 348,637 - GFF609 0.87 -2.0 348,763 - -2.5 348,823 + -3.2 348,884 - -2.4 348,888 - -1.8 348,888 - -3.7 348,888 - -2.3 348,963 + -2.9 348,987 - GFF610 0.11 -2.9 349,006 + GFF610 0.19 -1.9 349,079 - GFF610 0.51 -3.6 349,190 - -2.3 349,196 + -3.1 349,196 + -2.6 349,197 - -1.8 349,207 + -2.0 349,207 + -1.6 349,220 + -1.7 349,220 + -3.7 349,220 + -3.4 349,221 - +1.9 349,221 - +1.2 349,222 + -3.2 349,265 + -2.0 349,814 + +0.2 349,815 - +0.4 349,821 - -0.2 349,821 - -0.5 349,821 - -0.6
Or see this region's nucleotide sequence