Experiment: LB_plus_SM_buffer with EIP16_phage 0.0875 MOI
Only strains with sufficient reads to estimate fitness are shown,
but the strain fitness values are still rather noisy and may be biased towards zero.
Strains near the edge of a gene are not shown as being associated with that
gene (they are in grey).
Strains in the central 10-90% of a gene are color coded by the insertion's strand.
Usually, "+" means that the selectable marker is encoded on the forward strand,
i.e., transcribed rightward.
500 nt GFF601 and GFF602 are separated by 118 nucleotides GFF602 and GFF603 are separated by 376 nucleotides
GFF601 - 4-alpha-glucanotransferase (amylomaltase) (EC 2.4.1.25), at 339,176 to 341,254
GFF601
GFF602 - High-affinity gluconate transporter GntT, at 341,373 to 342,689
GFF602
GFF603 - NfuA Fe-S protein maturation, at 343,066 to 343,641
GFF603
Position (kb) 341
342
343 Strain fitness (log2 ratio) -1
0
1
2 at 340.432 kb on + strand, within GFF601 at 340.432 kb on + strand, within GFF601 at 340.432 kb on + strand, within GFF601 at 340.577 kb on + strand, within GFF601 at 340.577 kb on + strand, within GFF601 at 340.577 kb on + strand, within GFF601 at 340.577 kb on + strand, within GFF601 at 340.578 kb on - strand, within GFF601 at 340.609 kb on + strand, within GFF601 at 340.610 kb on - strand, within GFF601 at 340.642 kb on + strand, within GFF601 at 340.643 kb on - strand, within GFF601 at 340.643 kb on - strand, within GFF601 at 340.712 kb on + strand, within GFF601 at 340.713 kb on - strand, within GFF601 at 340.719 kb on - strand, within GFF601 at 340.721 kb on - strand, within GFF601 at 340.907 kb on + strand, within GFF601 at 340.907 kb on + strand, within GFF601 at 340.954 kb on + strand, within GFF601 at 340.955 kb on - strand, within GFF601 at 340.958 kb on + strand, within GFF601 at 340.958 kb on + strand, within GFF601 at 340.958 kb on + strand, within GFF601 at 340.959 kb on - strand, within GFF601 at 340.987 kb on + strand, within GFF601 at 341.019 kb on + strand, within GFF601 at 341.020 kb on - strand, within GFF601 at 341.043 kb on + strand, within GFF601 at 341.043 kb on + strand, within GFF601 at 341.196 kb on + strand at 341.297 kb on + strand at 341.358 kb on + strand at 341.358 kb on + strand at 341.451 kb on - strand at 341.664 kb on - strand, within GFF602 at 341.719 kb on + strand, within GFF602 at 341.719 kb on + strand, within GFF602 at 341.720 kb on - strand, within GFF602 at 341.720 kb on - strand, within GFF602 at 341.890 kb on - strand, within GFF602 at 342.173 kb on + strand, within GFF602 at 342.278 kb on - strand, within GFF602 at 342.283 kb on - strand, within GFF602 at 342.349 kb on + strand, within GFF602 at 342.350 kb on - strand, within GFF602 at 342.402 kb on - strand, within GFF602 at 342.651 kb on + strand at 342.660 kb on - strand at 342.668 kb on + strand at 342.826 kb on + strand at 342.865 kb on + strand at 342.952 kb on - strand at 343.004 kb on - strand at 343.071 kb on - strand at 343.071 kb on - strand at 343.071 kb on - strand at 343.071 kb on - strand at 343.073 kb on + strand at 343.073 kb on + strand at 343.073 kb on + strand at 343.195 kb on - strand, within GFF603 at 343.388 kb on - strand, within GFF603 at 343.427 kb on + strand, within GFF603 at 343.514 kb on + strand, within GFF603 at 343.514 kb on + strand, within GFF603 at 343.633 kb on - strand at 343.655 kb on + strand at 343.679 kb on - strand
Per-strain Table
Position Strand Gene LocusTag Fraction LB_plus_SM_buffer with EIP16_phage 0.0875 MOI remove 340,432 + GFF601 0.60 -1.0 340,432 + GFF601 0.60 +0.4 340,432 + GFF601 0.60 +0.7 340,577 + GFF601 0.67 -0.2 340,577 + GFF601 0.67 +0.5 340,577 + GFF601 0.67 +1.9 340,577 + GFF601 0.67 -0.1 340,578 - GFF601 0.67 +0.2 340,609 + GFF601 0.69 -0.0 340,610 - GFF601 0.69 -0.1 340,642 + GFF601 0.71 +0.3 340,643 - GFF601 0.71 +0.3 340,643 - GFF601 0.71 +0.8 340,712 + GFF601 0.74 +0.0 340,713 - GFF601 0.74 -0.5 340,719 - GFF601 0.74 -0.0 340,721 - GFF601 0.74 -0.1 340,907 + GFF601 0.83 +0.7 340,907 + GFF601 0.83 +0.2 340,954 + GFF601 0.86 -0.4 340,955 - GFF601 0.86 +0.1 340,958 + GFF601 0.86 +0.0 340,958 + GFF601 0.86 +0.1 340,958 + GFF601 0.86 -0.0 340,959 - GFF601 0.86 -0.3 340,987 + GFF601 0.87 +0.0 341,019 + GFF601 0.89 +0.7 341,020 - GFF601 0.89 +0.0 341,043 + GFF601 0.90 -0.2 341,043 + GFF601 0.90 +0.1 341,196 + +0.3 341,297 + -0.2 341,358 + +0.1 341,358 + +0.1 341,451 - +0.4 341,664 - GFF602 0.22 -0.1 341,719 + GFF602 0.26 -0.1 341,719 + GFF602 0.26 +0.1 341,720 - GFF602 0.26 +0.0 341,720 - GFF602 0.26 +0.3 341,890 - GFF602 0.39 -0.3 342,173 + GFF602 0.61 -0.4 342,278 - GFF602 0.69 +0.7 342,283 - GFF602 0.69 +0.5 342,349 + GFF602 0.74 +1.8 342,350 - GFF602 0.74 +0.1 342,402 - GFF602 0.78 +1.2 342,651 + +0.5 342,660 - +0.5 342,668 + -0.1 342,826 + +0.2 342,865 + -0.1 342,952 - -0.1 343,004 - -0.7 343,071 - +0.2 343,071 - -0.5 343,071 - -0.3 343,071 - -0.5 343,073 + -0.0 343,073 + +0.5 343,073 + -0.4 343,195 - GFF603 0.22 +1.4 343,388 - GFF603 0.56 +0.9 343,427 + GFF603 0.63 +0.1 343,514 + GFF603 0.78 +1.2 343,514 + GFF603 0.78 +1.3 343,633 - +0.8 343,655 + +0.7 343,679 - -0.3
Or see this region's nucleotide sequence