Experiment: LB_plus_SM_buffer with EIP16_phage 0.0875 MOI
Only strains with sufficient reads to estimate fitness are shown,
but the strain fitness values are still rather noisy and may be biased towards zero.
Strains near the edge of a gene are not shown as being associated with that
gene (they are in grey).
Strains in the central 10-90% of a gene are color coded by the insertion's strand.
Usually, "+" means that the selectable marker is encoded on the forward strand,
i.e., transcribed rightward.
500 nt GFF531 and GFF532 are separated by 3 nucleotides GFF532 and GFF533 are separated by 72 nucleotides GFF533 and GFF534 are separated by 4 nucleotides
GFF531 - Transporter, putative, at 263,638 to 264,855
GFF531
GFF532 - DcrB protein precursor, at 264,859 to 265,416
GFF532
GFF533 - Putative preQ0 transporter, at 265,489 to 266,229
GFF533
GFF534 - tRNA 5-methylaminomethyl-2-thiouridine synthase TusA, at 266,234 to 266,569
GFF534
Position (kb) 264
265
266 Strain fitness (log2 ratio) -1
0
1
2 at 263.864 kb on - strand, within GFF531 at 263.874 kb on - strand, within GFF531 at 263.874 kb on - strand, within GFF531 at 264.157 kb on + strand, within GFF531 at 264.225 kb on - strand, within GFF531 at 264.304 kb on + strand, within GFF531 at 264.358 kb on - strand, within GFF531 at 264.360 kb on + strand, within GFF531 at 264.378 kb on + strand, within GFF531 at 264.670 kb on + strand, within GFF531 at 264.671 kb on - strand, within GFF531 at 264.671 kb on - strand, within GFF531 at 264.671 kb on - strand, within GFF531 at 264.671 kb on - strand, within GFF531 at 264.864 kb on + strand at 264.865 kb on - strand at 264.876 kb on + strand at 264.877 kb on - strand at 265.035 kb on + strand, within GFF532 at 265.053 kb on + strand, within GFF532 at 265.054 kb on - strand, within GFF532 at 265.059 kb on + strand, within GFF532 at 265.060 kb on - strand, within GFF532 at 265.060 kb on - strand, within GFF532 at 265.093 kb on - strand, within GFF532 at 265.206 kb on + strand, within GFF532 at 265.344 kb on + strand, within GFF532 at 265.397 kb on + strand at 265.397 kb on + strand at 265.522 kb on - strand at 265.607 kb on + strand, within GFF533 at 265.644 kb on + strand, within GFF533 at 265.807 kb on - strand, within GFF533 at 265.883 kb on + strand, within GFF533 at 265.883 kb on + strand, within GFF533 at 265.884 kb on - strand, within GFF533 at 265.904 kb on + strand, within GFF533 at 265.904 kb on + strand, within GFF533 at 266.102 kb on - strand, within GFF533 at 266.102 kb on - strand, within GFF533 at 266.126 kb on - strand, within GFF533 at 266.142 kb on + strand, within GFF533 at 266.208 kb on + strand at 266.211 kb on + strand at 266.212 kb on - strand
Per-strain Table
Position Strand Gene LocusTag Fraction LB_plus_SM_buffer with EIP16_phage 0.0875 MOI remove 263,864 - GFF531 0.19 -1.1 263,874 - GFF531 0.19 -0.1 263,874 - GFF531 0.19 +0.0 264,157 + GFF531 0.43 -0.3 264,225 - GFF531 0.48 -0.1 264,304 + GFF531 0.55 -0.3 264,358 - GFF531 0.59 +0.3 264,360 + GFF531 0.59 -0.5 264,378 + GFF531 0.61 -0.3 264,670 + GFF531 0.85 +0.0 264,671 - GFF531 0.85 +0.2 264,671 - GFF531 0.85 +0.5 264,671 - GFF531 0.85 +1.0 264,671 - GFF531 0.85 -0.8 264,864 + -0.2 264,865 - -0.9 264,876 + +0.2 264,877 - +0.8 265,035 + GFF532 0.32 -0.6 265,053 + GFF532 0.35 -0.1 265,054 - GFF532 0.35 +0.6 265,059 + GFF532 0.36 +0.3 265,060 - GFF532 0.36 -0.6 265,060 - GFF532 0.36 +1.2 265,093 - GFF532 0.42 +0.6 265,206 + GFF532 0.62 -1.6 265,344 + GFF532 0.87 +0.8 265,397 + +0.4 265,397 + +0.1 265,522 - +0.2 265,607 + GFF533 0.16 +1.9 265,644 + GFF533 0.21 -0.4 265,807 - GFF533 0.43 +1.1 265,883 + GFF533 0.53 -0.0 265,883 + GFF533 0.53 +0.4 265,884 - GFF533 0.53 +0.4 265,904 + GFF533 0.56 -1.6 265,904 + GFF533 0.56 +0.3 266,102 - GFF533 0.83 -0.2 266,102 - GFF533 0.83 +0.4 266,126 - GFF533 0.86 -0.7 266,142 + GFF533 0.88 +0.5 266,208 + -0.0 266,211 + -0.1 266,212 - -0.1
Or see this region's nucleotide sequence