Experiment: LB_plus_SM_buffer with EIP16_phage 0.0875 MOI
Only strains with sufficient reads to estimate fitness are shown,
but the strain fitness values are still rather noisy and may be biased towards zero.
Strains near the edge of a gene are not shown as being associated with that
gene (they are in grey).
Strains in the central 10-90% of a gene are color coded by the insertion's strand.
Usually, "+" means that the selectable marker is encoded on the forward strand,
i.e., transcribed rightward.
500 nt GFF493 and GFF494 are separated by 173 nucleotides GFF494 and GFF495 are separated by 157 nucleotides
GFF493 - Cellulose synthase operon protein C, at 215,732 to 219,184
GFF493
GFF494 - Protein yhjK, at 219,358 to 221,364
GFF494
GFF495 - Aerobic C4-dicarboxylate transporter for fumarate, L-malate, D-malate, succunate, at 221,522 to 222,808
GFF495
Position (kb)
219
220
221
222 Strain fitness (log2 ratio)
-3
-2
-1
0
1
2 at 218.378 kb on - strand, within GFF493 at 218.408 kb on + strand, within GFF493 at 218.408 kb on + strand, within GFF493 at 218.409 kb on - strand, within GFF493 at 218.436 kb on - strand, within GFF493 at 218.634 kb on + strand, within GFF493 at 218.672 kb on + strand, within GFF493 at 218.672 kb on + strand, within GFF493 at 218.672 kb on + strand, within GFF493 at 218.672 kb on + strand, within GFF493 at 218.672 kb on + strand, within GFF493 at 218.675 kb on + strand, within GFF493 at 218.675 kb on + strand, within GFF493 at 218.675 kb on + strand, within GFF493 at 218.675 kb on + strand, within GFF493 at 218.676 kb on - strand, within GFF493 at 218.676 kb on - strand, within GFF493 at 218.676 kb on - strand, within GFF493 at 218.678 kb on - strand, within GFF493 at 218.781 kb on - strand, within GFF493 at 218.783 kb on + strand, within GFF493 at 218.783 kb on + strand, within GFF493 at 218.786 kb on - strand, within GFF493 at 218.799 kb on - strand, within GFF493 at 218.836 kb on + strand, within GFF493 at 218.859 kb on + strand at 219.081 kb on - strand at 219.104 kb on + strand at 219.182 kb on + strand at 219.286 kb on + strand at 219.286 kb on + strand at 219.319 kb on + strand at 219.385 kb on - strand at 219.476 kb on - strand at 219.582 kb on + strand, within GFF494 at 219.582 kb on + strand, within GFF494 at 219.583 kb on - strand, within GFF494 at 219.585 kb on + strand, within GFF494 at 219.620 kb on + strand, within GFF494 at 219.770 kb on - strand, within GFF494 at 219.777 kb on - strand, within GFF494 at 219.802 kb on + strand, within GFF494 at 219.802 kb on + strand, within GFF494 at 219.844 kb on + strand, within GFF494 at 219.849 kb on - strand, within GFF494 at 219.890 kb on + strand, within GFF494 at 220.027 kb on + strand, within GFF494 at 220.027 kb on + strand, within GFF494 at 220.028 kb on - strand, within GFF494 at 220.028 kb on - strand, within GFF494 at 220.048 kb on - strand, within GFF494 at 220.113 kb on + strand, within GFF494 at 220.146 kb on + strand, within GFF494 at 220.297 kb on - strand, within GFF494 at 220.367 kb on + strand, within GFF494 at 220.368 kb on - strand, within GFF494 at 220.379 kb on + strand, within GFF494 at 220.379 kb on + strand, within GFF494 at 220.387 kb on - strand, within GFF494 at 220.456 kb on + strand, within GFF494 at 220.456 kb on + strand, within GFF494 at 220.456 kb on + strand, within GFF494 at 220.457 kb on - strand, within GFF494 at 220.457 kb on - strand, within GFF494 at 220.457 kb on - strand, within GFF494 at 220.460 kb on - strand, within GFF494 at 220.486 kb on + strand, within GFF494 at 220.701 kb on + strand, within GFF494 at 220.702 kb on - strand, within GFF494 at 220.702 kb on - strand, within GFF494 at 220.702 kb on - strand, within GFF494 at 220.702 kb on - strand, within GFF494 at 221.005 kb on - strand, within GFF494 at 221.009 kb on + strand, within GFF494 at 221.009 kb on + strand, within GFF494 at 221.019 kb on - strand, within GFF494 at 221.066 kb on - strand, within GFF494 at 221.085 kb on - strand, within GFF494 at 221.092 kb on - strand, within GFF494 at 221.200 kb on - strand at 221.205 kb on - strand at 221.353 kb on + strand at 221.597 kb on + strand at 221.598 kb on - strand at 221.601 kb on - strand at 221.656 kb on + strand, within GFF495 at 221.657 kb on - strand, within GFF495 at 221.800 kb on + strand, within GFF495 at 221.908 kb on + strand, within GFF495 at 222.153 kb on - strand, within GFF495 at 222.153 kb on - strand, within GFF495 at 222.191 kb on - strand, within GFF495 at 222.200 kb on + strand, within GFF495 at 222.201 kb on - strand, within GFF495 at 222.201 kb on - strand, within GFF495 at 222.201 kb on - strand, within GFF495 at 222.202 kb on + strand, within GFF495 at 222.212 kb on - strand, within GFF495 at 222.284 kb on + strand, within GFF495 at 222.285 kb on - strand, within GFF495
Per-strain Table
Position Strand Gene LocusTag Fraction LB_plus_SM_buffer with EIP16_phage 0.0875 MOI remove 218,378 - GFF493 0.77 +1.4 218,408 + GFF493 0.77 +0.2 218,408 + GFF493 0.77 -0.0 218,409 - GFF493 0.78 -0.1 218,436 - GFF493 0.78 -1.9 218,634 + GFF493 0.84 +2.1 218,672 + GFF493 0.85 +0.3 218,672 + GFF493 0.85 +0.1 218,672 + GFF493 0.85 -0.5 218,672 + GFF493 0.85 -0.2 218,672 + GFF493 0.85 +0.2 218,675 + GFF493 0.85 -0.7 218,675 + GFF493 0.85 -0.8 218,675 + GFF493 0.85 +0.2 218,675 + GFF493 0.85 +0.1 218,676 - GFF493 0.85 -0.3 218,676 - GFF493 0.85 +0.2 218,676 - GFF493 0.85 -0.3 218,678 - GFF493 0.85 -0.7 218,781 - GFF493 0.88 -1.1 218,783 + GFF493 0.88 -0.3 218,783 + GFF493 0.88 -0.7 218,786 - GFF493 0.88 -0.5 218,799 - GFF493 0.89 -1.0 218,836 + GFF493 0.90 -0.2 218,859 + -0.9 219,081 - +0.3 219,104 + +1.2 219,182 + -0.1 219,286 + -0.0 219,286 + +1.3 219,319 + -1.0 219,385 - -2.0 219,476 - -2.7 219,582 + GFF494 0.11 -2.8 219,582 + GFF494 0.11 -0.1 219,583 - GFF494 0.11 -2.3 219,585 + GFF494 0.11 -1.1 219,620 + GFF494 0.13 -1.7 219,770 - GFF494 0.21 -1.5 219,777 - GFF494 0.21 -2.8 219,802 + GFF494 0.22 -2.2 219,802 + GFF494 0.22 +0.8 219,844 + GFF494 0.24 -1.9 219,849 - GFF494 0.24 -1.7 219,890 + GFF494 0.27 -2.0 220,027 + GFF494 0.33 -0.8 220,027 + GFF494 0.33 -1.6 220,028 - GFF494 0.33 -2.2 220,028 - GFF494 0.33 -2.1 220,048 - GFF494 0.34 -1.8 220,113 + GFF494 0.38 -0.1 220,146 + GFF494 0.39 -2.0 220,297 - GFF494 0.47 -2.4 220,367 + GFF494 0.50 -2.2 220,368 - GFF494 0.50 -0.4 220,379 + GFF494 0.51 -2.2 220,379 + GFF494 0.51 -2.3 220,387 - GFF494 0.51 -1.4 220,456 + GFF494 0.55 -0.1 220,456 + GFF494 0.55 -1.9 220,456 + GFF494 0.55 -0.8 220,457 - GFF494 0.55 -2.0 220,457 - GFF494 0.55 -2.1 220,457 - GFF494 0.55 -2.3 220,460 - GFF494 0.55 -1.5 220,486 + GFF494 0.56 -1.7 220,701 + GFF494 0.67 -2.3 220,702 - GFF494 0.67 -0.8 220,702 - GFF494 0.67 -0.2 220,702 - GFF494 0.67 -0.0 220,702 - GFF494 0.67 -1.9 221,005 - GFF494 0.82 +0.7 221,009 + GFF494 0.82 -0.8 221,009 + GFF494 0.82 +0.8 221,019 - GFF494 0.83 -1.5 221,066 - GFF494 0.85 -1.3 221,085 - GFF494 0.86 -1.9 221,092 - GFF494 0.86 -0.6 221,200 - -1.8 221,205 - +0.0 221,353 + +0.2 221,597 + -0.4 221,598 - -0.3 221,601 - -0.4 221,656 + GFF495 0.10 -0.5 221,657 - GFF495 0.10 -0.1 221,800 + GFF495 0.22 +0.2 221,908 + GFF495 0.30 +0.8 222,153 - GFF495 0.49 -0.1 222,153 - GFF495 0.49 +0.3 222,191 - GFF495 0.52 +0.4 222,200 + GFF495 0.53 -0.1 222,201 - GFF495 0.53 +0.6 222,201 - GFF495 0.53 -0.2 222,201 - GFF495 0.53 -0.1 222,202 + GFF495 0.53 -0.9 222,212 - GFF495 0.54 -1.2 222,284 + GFF495 0.59 -0.2 222,285 - GFF495 0.59 -0.3
Or see this region's nucleotide sequence