Experiment: LB_plus_SM_buffer with EIP16_phage 0.0875 MOI
Only strains with sufficient reads to estimate fitness are shown,
but the strain fitness values are still rather noisy and may be biased towards zero.
Strains near the edge of a gene are not shown as being associated with that
gene (they are in grey).
Strains in the central 10-90% of a gene are color coded by the insertion's strand.
Usually, "+" means that the selectable marker is encoded on the forward strand,
i.e., transcribed rightward.
500 nt GFF4599 and GFF4600 are separated by 184 nucleotides GFF4600 and GFF4601 are separated by 24 nucleotides GFF4601 and GFF4602 are separated by 114 nucleotides GFF4602 and GFF4603 are separated by 46 nucleotides
GFF4599 - Esterase ybfF (EC 3.1.-.-), at 388,780 to 389,550
GFF4599
GFF4600 - SeqA protein, negative modulator of initiation of replication, at 389,735 to 390,277
GFF4600
GFF4601 - Phosphoglucomutase (EC 5.4.2.2), at 390,302 to 391,942
GFF4601
GFF4602 - 'Pyridoxamine 5'-phosphate oxidase (EC 1.4.3.5)' transl_table=11, at 392,057 to 392,560
GFF4602
GFF4603 - Putrescine/proton symporter, putrescine/ornithine antiporter PotE, at 392,607 to 393,926
GFF4603
Position (kb) 390
391
392 Strain fitness (log2 ratio) -6
-5
-4
-3
-2
-1
0
1
2 at 389.386 kb on + strand, within GFF4599 at 389.505 kb on + strand at 389.589 kb on + strand at 389.590 kb on - strand at 389.623 kb on + strand at 389.630 kb on + strand at 389.687 kb on + strand at 389.771 kb on + strand at 389.772 kb on - strand at 389.827 kb on + strand, within GFF4600 at 390.142 kb on + strand, within GFF4600 at 390.143 kb on - strand, within GFF4600 at 390.143 kb on - strand, within GFF4600 at 390.143 kb on - strand, within GFF4600 at 390.276 kb on - strand at 390.276 kb on - strand at 390.287 kb on + strand at 390.287 kb on + strand at 390.380 kb on + strand at 390.380 kb on + strand at 390.381 kb on - strand at 390.384 kb on + strand at 390.545 kb on + strand, within GFF4601 at 390.546 kb on - strand, within GFF4601 at 390.546 kb on - strand, within GFF4601 at 390.546 kb on - strand, within GFF4601 at 390.652 kb on + strand, within GFF4601 at 390.722 kb on - strand, within GFF4601 at 390.731 kb on - strand, within GFF4601 at 390.766 kb on + strand, within GFF4601 at 390.766 kb on + strand, within GFF4601 at 390.767 kb on - strand, within GFF4601 at 390.767 kb on - strand, within GFF4601 at 390.915 kb on + strand, within GFF4601 at 391.072 kb on - strand, within GFF4601 at 391.072 kb on - strand, within GFF4601 at 391.072 kb on - strand, within GFF4601 at 391.105 kb on + strand, within GFF4601 at 391.288 kb on + strand, within GFF4601 at 391.289 kb on - strand, within GFF4601 at 391.293 kb on - strand, within GFF4601 at 391.534 kb on + strand, within GFF4601 at 391.634 kb on + strand, within GFF4601 at 392.137 kb on + strand, within GFF4602 at 392.137 kb on + strand, within GFF4602 at 392.138 kb on - strand, within GFF4602 at 392.138 kb on - strand, within GFF4602 at 392.266 kb on - strand, within GFF4602 at 392.270 kb on - strand, within GFF4602 at 392.270 kb on - strand, within GFF4602 at 392.271 kb on + strand, within GFF4602 at 392.294 kb on + strand, within GFF4602 at 392.295 kb on - strand, within GFF4602 at 392.295 kb on - strand, within GFF4602 at 392.295 kb on - strand, within GFF4602 at 392.298 kb on - strand, within GFF4602 at 392.346 kb on - strand, within GFF4602 at 392.513 kb on - strand at 392.581 kb on - strand at 392.654 kb on - strand at 392.656 kb on - strand at 392.732 kb on + strand at 392.734 kb on + strand at 392.734 kb on + strand at 392.735 kb on - strand at 392.836 kb on + strand, within GFF4603 at 392.843 kb on + strand, within GFF4603 at 392.843 kb on + strand, within GFF4603 at 392.844 kb on - strand, within GFF4603
Per-strain Table
Position Strand Gene LocusTag Fraction LB_plus_SM_buffer with EIP16_phage 0.0875 MOI remove 389,386 + GFF4599 0.79 -0.0 389,505 + -0.1 389,589 + -0.1 389,590 - +0.8 389,623 + +0.2 389,630 + -0.1 389,687 + +0.2 389,771 + -0.9 389,772 - +0.5 389,827 + GFF4600 0.17 -0.3 390,142 + GFF4600 0.75 -0.0 390,143 - GFF4600 0.75 -4.2 390,143 - GFF4600 0.75 -1.2 390,143 - GFF4600 0.75 -2.5 390,276 - -3.2 390,276 - -4.1 390,287 + -6.1 390,287 + -3.7 390,380 + -3.9 390,380 + -4.6 390,381 - -3.0 390,384 + -2.6 390,545 + GFF4601 0.15 -4.3 390,546 - GFF4601 0.15 -2.6 390,546 - GFF4601 0.15 -3.0 390,546 - GFF4601 0.15 -2.8 390,652 + GFF4601 0.21 -4.1 390,722 - GFF4601 0.26 -4.2 390,731 - GFF4601 0.26 -2.4 390,766 + GFF4601 0.28 -2.9 390,766 + GFF4601 0.28 -5.7 390,767 - GFF4601 0.28 -2.7 390,767 - GFF4601 0.28 -2.6 390,915 + GFF4601 0.37 -2.6 391,072 - GFF4601 0.47 -2.2 391,072 - GFF4601 0.47 -2.2 391,072 - GFF4601 0.47 -1.0 391,105 + GFF4601 0.49 -3.0 391,288 + GFF4601 0.60 -2.7 391,289 - GFF4601 0.60 -4.5 391,293 - GFF4601 0.60 -0.5 391,534 + GFF4601 0.75 -3.1 391,634 + GFF4601 0.81 -3.0 392,137 + GFF4602 0.16 +0.4 392,137 + GFF4602 0.16 -0.3 392,138 - GFF4602 0.16 +1.2 392,138 - GFF4602 0.16 +0.6 392,266 - GFF4602 0.41 +0.8 392,270 - GFF4602 0.42 -0.2 392,270 - GFF4602 0.42 +2.1 392,271 + GFF4602 0.42 +0.2 392,294 + GFF4602 0.47 -0.2 392,295 - GFF4602 0.47 +0.2 392,295 - GFF4602 0.47 +0.7 392,295 - GFF4602 0.47 -0.5 392,298 - GFF4602 0.48 +0.2 392,346 - GFF4602 0.57 +0.2 392,513 - +0.2 392,581 - -1.0 392,654 - +1.5 392,656 - -0.4 392,732 + +0.0 392,734 + +0.9 392,734 + -0.2 392,735 - -0.1 392,836 + GFF4603 0.17 -0.1 392,843 + GFF4603 0.18 +0.5 392,843 + GFF4603 0.18 -0.1 392,844 - GFF4603 0.18 +0.4
Or see this region's nucleotide sequence