Experiment: LB_plus_SM_buffer with EIP16_phage 0.0875 MOI
Only strains with sufficient reads to estimate fitness are shown,
but the strain fitness values are still rather noisy and may be biased towards zero.
Strains near the edge of a gene are not shown as being associated with that
gene (they are in grey).
Strains in the central 10-90% of a gene are color coded by the insertion's strand.
Usually, "+" means that the selectable marker is encoded on the forward strand,
i.e., transcribed rightward.
500 nt GFF4477 and GFF4478 are separated by 14 nucleotides GFF4478 and GFF4479 are separated by 2 nucleotides GFF4479 and GFF4480 are separated by 14 nucleotides GFF4480 and GFF4481 overlap by 23 nucleotides
GFF4477 - Glucosamine--fructose-6-phosphate aminotransferase [isomerizing] (EC 2.6.1.16), at 263,454 to 264,515
GFF4477
GFF4478 - PTS system, mannose-specific IID component (EC 2.7.1.69), at 264,530 to 265,384
GFF4478
GFF4479 - PTS system, mannose-specific IIC component (EC 2.7.1.69), at 265,387 to 266,130
GFF4479
GFF4480 - PTS system, mannose-specific IIB component (EC 2.7.1.69), at 266,145 to 266,615
GFF4480
GFF4481 - PTS system, mannose-specific IIA component (EC 2.7.1.69), at 266,593 to 267,042
GFF4481
Position (kb) 265
266
267 Strain fitness (log2 ratio) -1
0
1 at 264.395 kb on + strand, within GFF4477 at 264.396 kb on - strand, within GFF4477 at 264.497 kb on + strand at 264.498 kb on - strand at 264.499 kb on + strand at 264.499 kb on + strand at 264.500 kb on - strand at 264.508 kb on - strand at 264.508 kb on - strand at 264.515 kb on + strand at 264.516 kb on - strand at 264.516 kb on - strand at 264.522 kb on - strand at 264.622 kb on - strand, within GFF4478 at 264.622 kb on - strand, within GFF4478 at 264.789 kb on + strand, within GFF4478 at 264.790 kb on - strand, within GFF4478 at 264.951 kb on + strand, within GFF4478 at 264.951 kb on + strand, within GFF4478 at 264.952 kb on - strand, within GFF4478 at 264.952 kb on - strand, within GFF4478 at 265.258 kb on - strand, within GFF4478 at 265.280 kb on - strand, within GFF4478 at 265.480 kb on - strand, within GFF4479 at 265.511 kb on + strand, within GFF4479 at 265.511 kb on + strand, within GFF4479 at 265.512 kb on - strand, within GFF4479 at 265.512 kb on - strand, within GFF4479 at 265.530 kb on + strand, within GFF4479 at 265.531 kb on - strand, within GFF4479 at 265.603 kb on - strand, within GFF4479 at 265.603 kb on - strand, within GFF4479 at 265.743 kb on + strand, within GFF4479 at 265.827 kb on + strand, within GFF4479 at 265.834 kb on - strand, within GFF4479 at 265.879 kb on - strand, within GFF4479 at 266.130 kb on + strand at 266.154 kb on - strand at 266.159 kb on + strand at 266.257 kb on + strand, within GFF4480 at 266.258 kb on - strand, within GFF4480 at 266.260 kb on - strand, within GFF4480 at 266.363 kb on + strand, within GFF4480 at 266.478 kb on + strand, within GFF4480 at 266.479 kb on - strand, within GFF4480 at 266.487 kb on - strand, within GFF4480 at 266.604 kb on + strand at 266.630 kb on - strand at 266.653 kb on + strand, within GFF4481 at 266.700 kb on - strand, within GFF4481 at 266.936 kb on + strand, within GFF4481 at 266.991 kb on + strand, within GFF4481 at 267.030 kb on - strand at 267.030 kb on - strand
Per-strain Table
Position Strand Gene LocusTag Fraction LB_plus_SM_buffer with EIP16_phage 0.0875 MOI remove 264,395 + GFF4477 0.89 -0.3 264,396 - GFF4477 0.89 +0.3 264,497 + -0.9 264,498 - +1.3 264,499 + -1.3 264,499 + -0.0 264,500 - -0.1 264,508 - -0.4 264,508 - -0.8 264,515 + +0.3 264,516 - +0.2 264,516 - -0.8 264,522 - +0.1 264,622 - GFF4478 0.11 +0.0 264,622 - GFF4478 0.11 +0.2 264,789 + GFF4478 0.30 -0.1 264,790 - GFF4478 0.30 +0.2 264,951 + GFF4478 0.49 +0.2 264,951 + GFF4478 0.49 -0.2 264,952 - GFF4478 0.49 -0.0 264,952 - GFF4478 0.49 -0.8 265,258 - GFF4478 0.85 +0.4 265,280 - GFF4478 0.88 +0.9 265,480 - GFF4479 0.12 +1.1 265,511 + GFF4479 0.17 -0.5 265,511 + GFF4479 0.17 +1.7 265,512 - GFF4479 0.17 -0.1 265,512 - GFF4479 0.17 +0.4 265,530 + GFF4479 0.19 -0.1 265,531 - GFF4479 0.19 +0.7 265,603 - GFF4479 0.29 +0.4 265,603 - GFF4479 0.29 +0.4 265,743 + GFF4479 0.48 +0.5 265,827 + GFF4479 0.59 -0.0 265,834 - GFF4479 0.60 -0.7 265,879 - GFF4479 0.66 +0.2 266,130 + -0.6 266,154 - +1.0 266,159 + +0.1 266,257 + GFF4480 0.24 +0.4 266,258 - GFF4480 0.24 +0.1 266,260 - GFF4480 0.24 -0.4 266,363 + GFF4480 0.46 -0.6 266,478 + GFF4480 0.71 +0.4 266,479 - GFF4480 0.71 -1.4 266,487 - GFF4480 0.73 -0.0 266,604 + +0.9 266,630 - -0.4 266,653 + GFF4481 0.13 -0.2 266,700 - GFF4481 0.24 -0.7 266,936 + GFF4481 0.76 -0.3 266,991 + GFF4481 0.88 -0.3 267,030 - -0.1 267,030 - -0.1
Or see this region's nucleotide sequence