Experiment: LB_plus_SM_buffer with EIP16_phage 0.0875 MOI
Only strains with sufficient reads to estimate fitness are shown,
but the strain fitness values are still rather noisy and may be biased towards zero.
Strains near the edge of a gene are not shown as being associated with that
gene (they are in grey).
Strains in the central 10-90% of a gene are color coded by the insertion's strand.
Usually, "+" means that the selectable marker is encoded on the forward strand,
i.e., transcribed rightward.
500 nt GFF4414 and GFF4415 are separated by 185 nucleotides GFF4415 and GFF4416 are separated by 256 nucleotides
GFF4414 - FIG00809136: hypothetical protein, at 197,336 to 199,750
GFF4414
GFF4415 - Putative outer membrane protein, at 199,936 to 201,069
GFF4415
GFF4416 - Methionine ABC transporter substrate-binding protein, at 201,326 to 202,156
GFF4416
Position (kb)
199
200
201
202 Strain fitness (log2 ratio)
-1
0
1 at 199.107 kb on + strand, within GFF4414 at 199.135 kb on + strand, within GFF4414 at 199.419 kb on + strand, within GFF4414 at 199.434 kb on + strand, within GFF4414 at 199.434 kb on + strand, within GFF4414 at 199.485 kb on + strand, within GFF4414 at 199.486 kb on - strand, within GFF4414 at 199.728 kb on + strand at 199.916 kb on + strand at 200.137 kb on - strand, within GFF4415 at 200.219 kb on - strand, within GFF4415 at 200.221 kb on + strand, within GFF4415 at 200.222 kb on - strand, within GFF4415 at 200.289 kb on + strand, within GFF4415 at 200.309 kb on - strand, within GFF4415 at 200.309 kb on - strand, within GFF4415 at 200.369 kb on - strand, within GFF4415 at 200.518 kb on + strand, within GFF4415 at 200.545 kb on + strand, within GFF4415 at 200.648 kb on - strand, within GFF4415 at 200.648 kb on - strand, within GFF4415 at 200.698 kb on - strand, within GFF4415 at 200.880 kb on + strand, within GFF4415 at 200.914 kb on + strand, within GFF4415 at 200.914 kb on + strand, within GFF4415 at 201.098 kb on + strand at 201.166 kb on + strand at 201.312 kb on - strand at 201.423 kb on + strand, within GFF4416 at 201.483 kb on + strand, within GFF4416 at 201.483 kb on + strand, within GFF4416 at 201.499 kb on + strand, within GFF4416 at 201.550 kb on - strand, within GFF4416 at 201.550 kb on - strand, within GFF4416 at 201.671 kb on + strand, within GFF4416 at 201.785 kb on - strand, within GFF4416 at 201.793 kb on + strand, within GFF4416 at 201.802 kb on + strand, within GFF4416 at 201.803 kb on - strand, within GFF4416
Per-strain Table
Position Strand Gene LocusTag Fraction LB_plus_SM_buffer with EIP16_phage 0.0875 MOI remove 199,107 + GFF4414 0.73 -0.0 199,135 + GFF4414 0.74 -1.4 199,419 + GFF4414 0.86 -0.0 199,434 + GFF4414 0.87 -0.9 199,434 + GFF4414 0.87 -1.0 199,485 + GFF4414 0.89 +0.2 199,486 - GFF4414 0.89 +0.2 199,728 + +0.0 199,916 + -0.5 200,137 - GFF4415 0.18 +0.5 200,219 - GFF4415 0.25 -0.0 200,221 + GFF4415 0.25 +0.0 200,222 - GFF4415 0.25 +0.0 200,289 + GFF4415 0.31 +0.3 200,309 - GFF4415 0.33 -0.1 200,309 - GFF4415 0.33 +1.3 200,369 - GFF4415 0.38 +0.2 200,518 + GFF4415 0.51 +0.0 200,545 + GFF4415 0.54 +0.3 200,648 - GFF4415 0.63 +0.3 200,648 - GFF4415 0.63 -0.2 200,698 - GFF4415 0.67 -0.1 200,880 + GFF4415 0.83 +0.1 200,914 + GFF4415 0.86 +0.2 200,914 + GFF4415 0.86 +0.1 201,098 + -0.2 201,166 + +0.2 201,312 - -0.9 201,423 + GFF4416 0.12 -0.8 201,483 + GFF4416 0.19 -0.0 201,483 + GFF4416 0.19 +0.9 201,499 + GFF4416 0.21 +0.1 201,550 - GFF4416 0.27 +1.0 201,550 - GFF4416 0.27 -0.7 201,671 + GFF4416 0.42 +0.5 201,785 - GFF4416 0.55 +0.1 201,793 + GFF4416 0.56 +0.5 201,802 + GFF4416 0.57 -0.1 201,803 - GFF4416 0.57 +0.1
Or see this region's nucleotide sequence