Experiment: LB_plus_SM_buffer with EIP16_phage 0.0875 MOI
Only strains with sufficient reads to estimate fitness are shown,
but the strain fitness values are still rather noisy and may be biased towards zero.
Strains near the edge of a gene are not shown as being associated with that
gene (they are in grey).
Strains in the central 10-90% of a gene are color coded by the insertion's strand.
Usually, "+" means that the selectable marker is encoded on the forward strand,
i.e., transcribed rightward.
500 nt GFF4004 and GFF4005 are separated by 38 nucleotides GFF4005 and GFF4006 are separated by 281 nucleotides GFF4006 and GFF4007 are separated by 8 nucleotides GFF4007 and GFF4008 are separated by 9 nucleotides
GFF4004 - DsrB protein, at 276,567 to 276,764
GFF4004
GFF4005 - Colanic acid capsular biosynthesis activation accesory protein RcsA, co-regulator with RcsB, at 276,803 to 277,426
GFF4005
GFF4006 - Flagellar biosynthesis protein FliR, at 277,708 to 278,502
GFF4006
GFF4007 - Flagellar biosynthesis protein FliQ, at 278,511 to 278,780
GFF4007
GFF4008 - Flagellar biosynthesis protein FliP, at 278,790 to 279,527
GFF4008
Position (kb) 277
278
279 Strain fitness (log2 ratio) -1
0
1 at 276.762 kb on + strand at 276.855 kb on - strand at 276.862 kb on - strand at 277.395 kb on + strand at 278.099 kb on - strand, within GFF4006 at 278.113 kb on - strand, within GFF4006 at 278.118 kb on + strand, within GFF4006 at 278.139 kb on + strand, within GFF4006 at 278.457 kb on - strand at 278.472 kb on - strand at 278.473 kb on + strand at 278.522 kb on + strand at 278.525 kb on - strand at 278.554 kb on + strand, within GFF4007 at 278.594 kb on + strand, within GFF4007 at 278.707 kb on - strand, within GFF4007 at 279.262 kb on - strand, within GFF4008
Per-strain Table
Position Strand Gene LocusTag Fraction LB_plus_SM_buffer with EIP16_phage 0.0875 MOI remove 276,762 + +0.2 276,855 - -0.6 276,862 - -0.1 277,395 + -0.6 278,099 - GFF4006 0.49 +1.1 278,113 - GFF4006 0.51 +0.9 278,118 + GFF4006 0.52 +1.1 278,139 + GFF4006 0.54 +0.9 278,457 - -0.0 278,472 - +0.8 278,473 + +1.5 278,522 + +0.9 278,525 - +1.2 278,554 + GFF4007 0.16 -0.2 278,594 + GFF4007 0.31 +1.2 278,707 - GFF4007 0.73 +0.9 279,262 - GFF4008 0.64 +1.1
Or see this region's nucleotide sequence