Experiment: LB_plus_SM_buffer with EIP16_phage 0.0875 MOI
Only strains with sufficient reads to estimate fitness are shown,
but the strain fitness values are still rather noisy and may be biased towards zero.
Strains near the edge of a gene are not shown as being associated with that
gene (they are in grey).
Strains in the central 10-90% of a gene are color coded by the insertion's strand.
Usually, "+" means that the selectable marker is encoded on the forward strand,
i.e., transcribed rightward.
500 nt GFF3437 and GFF3438 are separated by 91 nucleotides GFF3438 and GFF3439 are separated by 145 nucleotides GFF3439 and GFF3440 are separated by 142 nucleotides GFF3440 and GFF3441 overlap by 1 nucleotides GFF3441 and GFF3442 overlap by 11 nucleotides
GFF3437 - Putative outer membrane lipoprotein, at 200,179 to 200,493
GFF3437
GFF3438 - FIG01046842: hypothetical protein, at 200,585 to 200,743
GFF3438
GFF3439 - hypothetical protein, at 200,889 to 201,032
GFF3439
GFF3440 - Nickel ABC transporter, periplasmic nickel-binding protein nikA2 (TC 3.A.1.5.3), at 201,175 to 202,809
GFF3440
GFF3441 - Nickel transport system permease protein nikB2 (TC 3.A.1.5.3), at 202,809 to 203,783
GFF3441
GFF3442 - Nickel transport system permease protein nikC2 (TC 3.A.1.5.3), at 203,773 to 204,585
GFF3442
Position (kb) 201
202
203 Strain fitness (log2 ratio) -1
0
1
2 at 200.292 kb on + strand, within GFF3437 at 200.311 kb on + strand, within GFF3437 at 200.311 kb on + strand, within GFF3437 at 200.311 kb on + strand, within GFF3437 at 200.312 kb on - strand, within GFF3437 at 200.508 kb on - strand at 200.788 kb on - strand at 200.883 kb on + strand at 200.884 kb on - strand at 200.900 kb on + strand at 200.951 kb on + strand, within GFF3439 at 200.985 kb on + strand, within GFF3439 at 201.175 kb on + strand at 201.207 kb on - strand at 201.363 kb on + strand, within GFF3440 at 201.363 kb on + strand, within GFF3440 at 201.363 kb on + strand, within GFF3440 at 201.388 kb on + strand, within GFF3440 at 201.501 kb on - strand, within GFF3440 at 201.536 kb on + strand, within GFF3440 at 201.567 kb on + strand, within GFF3440 at 201.568 kb on - strand, within GFF3440 at 201.609 kb on + strand, within GFF3440 at 201.811 kb on - strand, within GFF3440 at 201.933 kb on + strand, within GFF3440 at 201.934 kb on - strand, within GFF3440 at 201.934 kb on - strand, within GFF3440 at 201.963 kb on + strand, within GFF3440 at 202.114 kb on - strand, within GFF3440 at 202.159 kb on + strand, within GFF3440 at 202.162 kb on - strand, within GFF3440 at 202.499 kb on - strand, within GFF3440 at 202.528 kb on + strand, within GFF3440 at 202.528 kb on + strand, within GFF3440 at 202.583 kb on + strand, within GFF3440 at 202.583 kb on + strand, within GFF3440 at 202.584 kb on - strand, within GFF3440 at 202.702 kb on - strand at 202.705 kb on + strand at 202.705 kb on + strand at 202.886 kb on + strand at 202.920 kb on - strand, within GFF3441 at 203.227 kb on + strand, within GFF3441 at 203.228 kb on - strand, within GFF3441 at 203.297 kb on - strand, within GFF3441 at 203.384 kb on - strand, within GFF3441 at 203.392 kb on + strand, within GFF3441 at 203.393 kb on - strand, within GFF3441 at 203.510 kb on - strand, within GFF3441 at 203.524 kb on + strand, within GFF3441 at 203.524 kb on + strand, within GFF3441 at 203.524 kb on + strand, within GFF3441 at 203.524 kb on + strand, within GFF3441 at 203.525 kb on - strand, within GFF3441 at 203.554 kb on + strand, within GFF3441 at 203.689 kb on + strand at 203.701 kb on + strand at 203.701 kb on + strand at 203.702 kb on - strand at 203.705 kb on - strand at 203.705 kb on - strand
Per-strain Table
Position Strand Gene LocusTag Fraction LB_plus_SM_buffer with EIP16_phage 0.0875 MOI remove 200,292 + GFF3437 0.36 +0.6 200,311 + GFF3437 0.42 -0.3 200,311 + GFF3437 0.42 +0.1 200,311 + GFF3437 0.42 -0.1 200,312 - GFF3437 0.42 -0.4 200,508 - +0.1 200,788 - -0.0 200,883 + +0.2 200,884 - +1.3 200,900 + -0.9 200,951 + GFF3439 0.43 -0.5 200,985 + GFF3439 0.67 +0.3 201,175 + +0.1 201,207 - +0.4 201,363 + GFF3440 0.11 +0.9 201,363 + GFF3440 0.11 -1.0 201,363 + GFF3440 0.11 -0.2 201,388 + GFF3440 0.13 +0.3 201,501 - GFF3440 0.20 -0.1 201,536 + GFF3440 0.22 -1.2 201,567 + GFF3440 0.24 +0.6 201,568 - GFF3440 0.24 -0.6 201,609 + GFF3440 0.27 -0.1 201,811 - GFF3440 0.39 +0.0 201,933 + GFF3440 0.46 -0.3 201,934 - GFF3440 0.46 +0.8 201,934 - GFF3440 0.46 +1.3 201,963 + GFF3440 0.48 +0.1 202,114 - GFF3440 0.57 +2.0 202,159 + GFF3440 0.60 -0.2 202,162 - GFF3440 0.60 -0.2 202,499 - GFF3440 0.81 +2.4 202,528 + GFF3440 0.83 +0.0 202,528 + GFF3440 0.83 +1.0 202,583 + GFF3440 0.86 -0.1 202,583 + GFF3440 0.86 +0.2 202,584 - GFF3440 0.86 +0.7 202,702 - -0.0 202,705 + -0.0 202,705 + -0.3 202,886 + -0.3 202,920 - GFF3441 0.11 +0.4 203,227 + GFF3441 0.43 -0.1 203,228 - GFF3441 0.43 -0.9 203,297 - GFF3441 0.50 -0.2 203,384 - GFF3441 0.59 +0.6 203,392 + GFF3441 0.60 +0.0 203,393 - GFF3441 0.60 -0.9 203,510 - GFF3441 0.72 -0.0 203,524 + GFF3441 0.73 +0.4 203,524 + GFF3441 0.73 +0.8 203,524 + GFF3441 0.73 -0.3 203,524 + GFF3441 0.73 -0.2 203,525 - GFF3441 0.73 -0.5 203,554 + GFF3441 0.76 -0.0 203,689 + +0.3 203,701 + +0.3 203,701 + -0.6 203,702 - +0.2 203,705 - +0.3 203,705 - +0.4
Or see this region's nucleotide sequence