Experiment: LB_plus_SM_buffer with EIP16_phage 0.0875 MOI
Only strains with sufficient reads to estimate fitness are shown,
but the strain fitness values are still rather noisy and may be biased towards zero.
Strains near the edge of a gene are not shown as being associated with that
gene (they are in grey).
Strains in the central 10-90% of a gene are color coded by the insertion's strand.
Usually, "+" means that the selectable marker is encoded on the forward strand,
i.e., transcribed rightward.
500 nt GFF3301 and GFF3302 are separated by 60 nucleotides GFF3302 and GFF3303 are separated by 229 nucleotides GFF3303 and GFF3304 are separated by 892 nucleotides
GFF3301 - Transcriptional repressor of PutA and PutP / Proline dehydrogenase (EC 1.5.99.8) (Proline oxidase) / Delta-1-pyrroline-5-carboxylate dehydrogenase (EC 1.2.1.88), at 69,463 to 73,425
GFF3301
GFF3302 - FIG00639374: hypothetical protein, at 73,486 to 73,617
GFF3302
GFF3303 - Proline/sodium symporter PutP (TC 2.A.21.2.1) @ Propionate/sodium symporter, at 73,847 to 75,355
GFF3303
GFF3304 - Phosphate starvation-inducible protein PhoH, predicted ATPase, at 76,248 to 77,036
GFF3304
Position (kb)
73
74
75
76 Strain fitness (log2 ratio)
-1
0
1 at 72.874 kb on + strand, within GFF3301 at 72.889 kb on - strand, within GFF3301 at 73.268 kb on - strand at 73.287 kb on + strand at 73.307 kb on + strand at 73.308 kb on - strand at 73.311 kb on + strand at 73.615 kb on + strand at 73.769 kb on + strand at 73.769 kb on + strand at 73.769 kb on + strand at 73.769 kb on + strand at 73.769 kb on + strand at 73.769 kb on + strand at 73.770 kb on - strand at 73.852 kb on + strand at 73.856 kb on - strand at 73.949 kb on + strand at 74.011 kb on + strand, within GFF3303 at 74.161 kb on + strand, within GFF3303 at 74.206 kb on + strand, within GFF3303 at 74.207 kb on - strand, within GFF3303 at 74.207 kb on - strand, within GFF3303 at 74.260 kb on + strand, within GFF3303 at 74.264 kb on + strand, within GFF3303 at 74.264 kb on + strand, within GFF3303 at 74.265 kb on - strand, within GFF3303 at 74.461 kb on + strand, within GFF3303 at 74.588 kb on + strand, within GFF3303 at 74.588 kb on + strand, within GFF3303 at 74.606 kb on - strand, within GFF3303 at 74.916 kb on + strand, within GFF3303 at 74.917 kb on - strand, within GFF3303 at 74.921 kb on - strand, within GFF3303 at 75.247 kb on - strand at 75.411 kb on - strand at 75.432 kb on - strand at 75.554 kb on - strand at 75.556 kb on - strand at 75.556 kb on - strand at 75.576 kb on + strand at 75.602 kb on - strand at 75.912 kb on + strand at 75.917 kb on - strand at 75.922 kb on + strand at 75.925 kb on + strand at 76.009 kb on - strand at 76.106 kb on + strand at 76.106 kb on + strand at 76.107 kb on - strand at 76.153 kb on - strand at 76.160 kb on - strand at 76.222 kb on + strand at 76.223 kb on - strand at 76.223 kb on - strand at 76.224 kb on + strand at 76.228 kb on + strand at 76.229 kb on - strand at 76.322 kb on + strand
Per-strain Table
Position Strand Gene LocusTag Fraction LB_plus_SM_buffer with EIP16_phage 0.0875 MOI remove 72,874 + GFF3301 0.86 +0.6 72,889 - GFF3301 0.86 -0.8 73,268 - -0.0 73,287 + -0.0 73,307 + +0.3 73,308 - +0.5 73,311 + -1.5 73,615 + +0.3 73,769 + +0.3 73,769 + +0.0 73,769 + +0.5 73,769 + +0.4 73,769 + -0.1 73,769 + -0.3 73,770 - -0.4 73,852 + -0.8 73,856 - -1.2 73,949 + +0.0 74,011 + GFF3303 0.11 -0.2 74,161 + GFF3303 0.21 +0.1 74,206 + GFF3303 0.24 +0.4 74,207 - GFF3303 0.24 +0.2 74,207 - GFF3303 0.24 +1.5 74,260 + GFF3303 0.27 +0.0 74,264 + GFF3303 0.28 +0.0 74,264 + GFF3303 0.28 +0.1 74,265 - GFF3303 0.28 -0.1 74,461 + GFF3303 0.41 +0.2 74,588 + GFF3303 0.49 +0.3 74,588 + GFF3303 0.49 +0.8 74,606 - GFF3303 0.50 +0.1 74,916 + GFF3303 0.71 -0.1 74,917 - GFF3303 0.71 +1.7 74,921 - GFF3303 0.71 -0.9 75,247 - -0.1 75,411 - +0.8 75,432 - -0.4 75,554 - +0.1 75,556 - +0.5 75,556 - +0.3 75,576 + +0.8 75,602 - -0.3 75,912 + +0.1 75,917 - -0.7 75,922 + -0.3 75,925 + +0.4 76,009 - +0.0 76,106 + -0.3 76,106 + +0.6 76,107 - +0.0 76,153 - +0.3 76,160 - -0.0 76,222 + +0.2 76,223 - -0.4 76,223 - -0.1 76,224 + +0.1 76,228 + -0.4 76,229 - -0.0 76,322 + -0.6
Or see this region's nucleotide sequence