Experiment: LB_plus_SM_buffer with EIP16_phage 0.0875 MOI
Only strains with sufficient reads to estimate fitness are shown,
but the strain fitness values are still rather noisy and may be biased towards zero.
Strains near the edge of a gene are not shown as being associated with that
gene (they are in grey).
Strains in the central 10-90% of a gene are color coded by the insertion's strand.
Usually, "+" means that the selectable marker is encoded on the forward strand,
i.e., transcribed rightward.
500 nt GFF3246 and GFF3247 are separated by 185 nucleotides GFF3247 and GFF3248 are separated by 71 nucleotides GFF3248 and GFF3249 are separated by 332 nucleotides
GFF3246 - ATP-dependent protease La (EC 3.4.21.53) Type II, at 20,361 to 22,121
GFF3246
GFF3247 - Macrodomain Ter protein YcbG, at 22,307 to 22,759
GFF3247
GFF3248 - Outer membrane protein A precursor, at 22,831 to 23,907
GFF3248
GFF3249 - Cell division inhibitor, at 24,240 to 24,749
GFF3249
Position (kb)
22
23
24 Strain fitness (log2 ratio)
-1
0
1 at 21.938 kb on + strand, within GFF3246 at 21.972 kb on + strand at 21.973 kb on - strand at 22.020 kb on + strand at 22.021 kb on - strand at 22.060 kb on - strand at 22.069 kb on + strand at 22.145 kb on + strand at 22.166 kb on - strand at 22.397 kb on + strand, within GFF3247 at 22.489 kb on + strand, within GFF3247 at 23.067 kb on - strand, within GFF3248 at 23.243 kb on - strand, within GFF3248 at 23.318 kb on - strand, within GFF3248 at 23.473 kb on - strand, within GFF3248 at 23.519 kb on - strand, within GFF3248 at 23.605 kb on - strand, within GFF3248 at 23.658 kb on - strand, within GFF3248 at 23.831 kb on - strand at 23.988 kb on - strand at 23.988 kb on - strand at 23.992 kb on - strand at 24.022 kb on - strand at 24.036 kb on - strand at 24.048 kb on - strand at 24.093 kb on + strand at 24.121 kb on - strand at 24.181 kb on + strand at 24.339 kb on + strand, within GFF3249 at 24.405 kb on + strand, within GFF3249 at 24.406 kb on - strand, within GFF3249 at 24.416 kb on - strand, within GFF3249 at 24.426 kb on - strand, within GFF3249 at 24.580 kb on - strand, within GFF3249 at 24.746 kb on - strand at 24.749 kb on + strand at 24.750 kb on - strand at 24.750 kb on - strand
Per-strain Table
Position Strand Gene LocusTag Fraction LB_plus_SM_buffer with EIP16_phage 0.0875 MOI remove 21,938 + GFF3246 0.90 +0.6 21,972 + +0.4 21,973 - +0.0 22,020 + +1.1 22,021 - +1.5 22,060 - +0.2 22,069 + +1.7 22,145 + +0.1 22,166 - +0.1 22,397 + GFF3247 0.20 -0.7 22,489 + GFF3247 0.40 -0.7 23,067 - GFF3248 0.22 +0.3 23,243 - GFF3248 0.38 -1.1 23,318 - GFF3248 0.45 -0.2 23,473 - GFF3248 0.60 -0.5 23,519 - GFF3248 0.64 -0.8 23,605 - GFF3248 0.72 -0.8 23,658 - GFF3248 0.77 -0.9 23,831 - -0.8 23,988 - +1.1 23,988 - +0.8 23,992 - +0.9 24,022 - +0.6 24,036 - +0.9 24,048 - +0.8 24,093 + +0.2 24,121 - +0.3 24,181 + +0.4 24,339 + GFF3249 0.19 +0.5 24,405 + GFF3249 0.32 +0.4 24,406 - GFF3249 0.33 +0.5 24,416 - GFF3249 0.35 +0.3 24,426 - GFF3249 0.36 -0.1 24,580 - GFF3249 0.67 +0.5 24,746 - +0.2 24,749 + -0.6 24,750 - +0.1 24,750 - -0.5
Or see this region's nucleotide sequence