Experiment: LB_plus_SM_buffer with EIP16_phage 0.0875 MOI
Only strains with sufficient reads to estimate fitness are shown,
but the strain fitness values are still rather noisy and may be biased towards zero.
Strains near the edge of a gene are not shown as being associated with that
gene (they are in grey).
Strains in the central 10-90% of a gene are color coded by the insertion's strand.
Usually, "+" means that the selectable marker is encoded on the forward strand,
i.e., transcribed rightward.
500 nt GFF3241 and GFF3242 are separated by 4 nucleotides GFF3242 and GFF3243 overlap by 4 nucleotides GFF3243 and GFF3244 overlap by 46 nucleotides
GFF3241 - Paraquat-inducible protein A, at 15,793 to 17,046
GFF3241
GFF3242 - Paraquat-inducible protein B, at 17,051 to 18,691
GFF3242
GFF3243 - Paraquat-inducible protein B, at 18,688 to 19,251
GFF3243
GFF3244 - hypothetical protein, at 19,206 to 19,343
GFF3244
Position (kb) 17
18
19 Strain fitness (log2 ratio) -1
0
1
2 at 16.152 kb on - strand, within GFF3241 at 16.314 kb on + strand, within GFF3241 at 16.528 kb on + strand, within GFF3241 at 16.529 kb on - strand, within GFF3241 at 16.728 kb on + strand, within GFF3241 at 16.955 kb on + strand at 17.080 kb on - strand at 17.156 kb on + strand at 17.162 kb on + strand at 17.431 kb on + strand, within GFF3242 at 17.663 kb on - strand, within GFF3242 at 17.878 kb on + strand, within GFF3242 at 17.977 kb on + strand, within GFF3242 at 18.033 kb on + strand, within GFF3242 at 18.053 kb on - strand, within GFF3242 at 18.198 kb on - strand, within GFF3242 at 18.198 kb on - strand, within GFF3242 at 18.260 kb on + strand, within GFF3242 at 18.398 kb on - strand, within GFF3242 at 18.398 kb on - strand, within GFF3242 at 18.430 kb on + strand, within GFF3242 at 18.430 kb on + strand, within GFF3242 at 18.431 kb on - strand, within GFF3242 at 18.442 kb on + strand, within GFF3242 at 18.542 kb on + strand at 18.543 kb on - strand at 18.656 kb on - strand at 18.760 kb on + strand, within GFF3243 at 18.763 kb on + strand, within GFF3243 at 18.824 kb on + strand, within GFF3243 at 18.824 kb on + strand, within GFF3243 at 18.845 kb on + strand, within GFF3243 at 18.853 kb on + strand, within GFF3243 at 18.898 kb on + strand, within GFF3243 at 18.899 kb on - strand, within GFF3243 at 19.168 kb on + strand, within GFF3243 at 19.261 kb on - strand, within GFF3244 at 19.261 kb on - strand, within GFF3244 at 19.368 kb on + strand at 19.430 kb on + strand at 19.490 kb on + strand at 19.555 kb on + strand
Per-strain Table
Position Strand Gene LocusTag Fraction LB_plus_SM_buffer with EIP16_phage 0.0875 MOI remove 16,152 - GFF3241 0.29 +0.2 16,314 + GFF3241 0.42 +0.2 16,528 + GFF3241 0.59 -0.1 16,529 - GFF3241 0.59 +0.5 16,728 + GFF3241 0.75 -0.1 16,955 + -0.4 17,080 - -0.8 17,156 + -1.3 17,162 + +1.0 17,431 + GFF3242 0.23 +0.4 17,663 - GFF3242 0.37 +0.1 17,878 + GFF3242 0.50 +0.3 17,977 + GFF3242 0.56 -0.4 18,033 + GFF3242 0.60 +0.0 18,053 - GFF3242 0.61 +0.0 18,198 - GFF3242 0.70 -0.5 18,198 - GFF3242 0.70 +0.1 18,260 + GFF3242 0.74 -0.4 18,398 - GFF3242 0.82 -0.7 18,398 - GFF3242 0.82 -0.0 18,430 + GFF3242 0.84 +0.0 18,430 + GFF3242 0.84 +0.4 18,431 - GFF3242 0.84 +2.4 18,442 + GFF3242 0.85 +0.1 18,542 + -0.1 18,543 - -0.4 18,656 - +0.8 18,760 + GFF3243 0.13 +0.5 18,763 + GFF3243 0.13 +0.4 18,824 + GFF3243 0.24 -0.1 18,824 + GFF3243 0.24 +0.3 18,845 + GFF3243 0.28 +0.1 18,853 + GFF3243 0.29 -0.1 18,898 + GFF3243 0.37 +0.0 18,899 - GFF3243 0.37 +0.1 19,168 + GFF3243 0.85 +0.4 19,261 - GFF3244 0.40 -0.3 19,261 - GFF3244 0.40 +0.1 19,368 + -0.8 19,430 + -0.2 19,490 + +0.4 19,555 + -0.4
Or see this region's nucleotide sequence