Experiment: LB_plus_SM_buffer with EIP16_phage 0.0875 MOI
Only strains with sufficient reads to estimate fitness are shown,
but the strain fitness values are still rather noisy and may be biased towards zero.
Strains near the edge of a gene are not shown as being associated with that
gene (they are in grey).
Strains in the central 10-90% of a gene are color coded by the insertion's strand.
Usually, "+" means that the selectable marker is encoded on the forward strand,
i.e., transcribed rightward.
500 nt GFF3010 and GFF3011 are separated by 166 nucleotides GFF3011 and GFF3012 are separated by 245 nucleotides GFF3012 and GFF3013 are separated by 380 nucleotides
GFF3010 - UPF0149 exported protein YgfB, at 26,116 to 26,694
GFF3010
GFF3011 - Z-ring-associated protein ZapA, at 26,861 to 27,190
GFF3011
GFF3012 - 5-formyltetrahydrofolate cyclo-ligase (EC 6.3.3.2), at 27,436 to 28,032
GFF3012
GFF3013 - D-3-phosphoglycerate dehydrogenase (EC 1.1.1.95), at 28,413 to 29,645
GFF3013
Position (kb) 27
28
29 Strain fitness (log2 ratio) -3
-2
-1
0
1
2
3 at 26.667 kb on - strand at 26.688 kb on + strand at 26.689 kb on - strand at 26.689 kb on - strand at 26.689 kb on - strand at 26.689 kb on - strand at 26.694 kb on + strand at 26.694 kb on + strand at 26.695 kb on - strand at 26.695 kb on - strand at 26.719 kb on + strand at 26.719 kb on + strand at 26.720 kb on - strand at 26.729 kb on - strand at 26.787 kb on - strand at 26.790 kb on + strand at 26.793 kb on - strand at 26.889 kb on + strand at 27.204 kb on - strand at 27.207 kb on - strand at 27.212 kb on - strand at 27.651 kb on - strand, within GFF3012 at 27.724 kb on + strand, within GFF3012 at 27.891 kb on + strand, within GFF3012 at 27.891 kb on + strand, within GFF3012 at 27.892 kb on - strand, within GFF3012 at 27.915 kb on + strand, within GFF3012 at 27.916 kb on - strand, within GFF3012 at 27.923 kb on + strand, within GFF3012 at 27.985 kb on - strand at 28.125 kb on + strand at 28.125 kb on + strand at 28.128 kb on + strand at 28.173 kb on + strand at 28.173 kb on + strand at 28.200 kb on - strand at 28.258 kb on - strand at 28.268 kb on + strand at 28.276 kb on + strand at 28.298 kb on - strand at 28.301 kb on + strand at 28.302 kb on - strand at 28.333 kb on + strand at 28.333 kb on + strand at 28.333 kb on + strand at 28.334 kb on - strand at 28.498 kb on - strand at 28.507 kb on - strand at 28.513 kb on + strand at 28.557 kb on - strand, within GFF3013 at 28.706 kb on + strand, within GFF3013 at 28.706 kb on + strand, within GFF3013 at 28.707 kb on - strand, within GFF3013 at 28.707 kb on - strand, within GFF3013 at 28.707 kb on - strand, within GFF3013 at 28.829 kb on + strand, within GFF3013 at 28.915 kb on - strand, within GFF3013
Per-strain Table
Position Strand Gene LocusTag Fraction LB_plus_SM_buffer with EIP16_phage 0.0875 MOI remove 26,667 - -1.0 26,688 + -0.6 26,689 - -0.4 26,689 - -0.7 26,689 - -0.1 26,689 - -0.7 26,694 + +0.1 26,694 + -0.2 26,695 - -0.2 26,695 - -0.2 26,719 + +0.0 26,719 + -1.8 26,720 - -0.0 26,729 - +0.1 26,787 - -0.2 26,790 + -1.1 26,793 - -0.2 26,889 + -0.3 27,204 - -0.6 27,207 - -0.2 27,212 - -1.0 27,651 - GFF3012 0.36 -3.7 27,724 + GFF3012 0.48 -3.5 27,891 + GFF3012 0.76 +0.8 27,891 + GFF3012 0.76 -0.8 27,892 - GFF3012 0.76 -0.0 27,915 + GFF3012 0.80 -1.9 27,916 - GFF3012 0.80 -2.3 27,923 + GFF3012 0.82 -1.3 27,985 - -1.2 28,125 + -0.4 28,125 + +0.4 28,128 + -1.4 28,173 + -0.9 28,173 + +0.0 28,200 - +0.5 28,258 - -0.2 28,268 + +1.1 28,276 + -0.2 28,298 - +0.1 28,301 + +3.5 28,302 - +0.3 28,333 + -0.3 28,333 + +0.2 28,333 + +0.3 28,334 - +1.2 28,498 - +0.4 28,507 - +0.4 28,513 + +0.4 28,557 - GFF3013 0.12 +0.4 28,706 + GFF3013 0.24 +0.0 28,706 + GFF3013 0.24 +0.4 28,707 - GFF3013 0.24 +0.7 28,707 - GFF3013 0.24 +0.0 28,707 - GFF3013 0.24 +0.5 28,829 + GFF3013 0.34 +0.7 28,915 - GFF3013 0.41 +0.0
Or see this region's nucleotide sequence