Experiment: LB_plus_SM_buffer with EIP16_phage 0.0875 MOI
Only strains with sufficient reads to estimate fitness are shown,
but the strain fitness values are still rather noisy and may be biased towards zero.
Strains near the edge of a gene are not shown as being associated with that
gene (they are in grey).
Strains in the central 10-90% of a gene are color coded by the insertion's strand.
Usually, "+" means that the selectable marker is encoded on the forward strand,
i.e., transcribed rightward.
500 nt GFF296 and GFF297 are separated by 226 nucleotides
GFF296 - Multidrug resistance protein D, at 12,205 to 13,365
GFF296
GFF297 - Integral membrane protein, at 13,592 to 14,452
GFF297
Position (kb) 13
14
15 Strain fitness (log2 ratio) -2
-1
0
1
2 at 12.663 kb on + strand, within GFF296 at 12.676 kb on + strand, within GFF296 at 12.676 kb on + strand, within GFF296 at 12.676 kb on + strand, within GFF296 at 12.677 kb on - strand, within GFF296 at 12.680 kb on - strand, within GFF296 at 12.755 kb on + strand, within GFF296 at 12.790 kb on - strand, within GFF296 at 12.791 kb on + strand, within GFF296 at 12.979 kb on + strand, within GFF296 at 12.979 kb on + strand, within GFF296 at 13.017 kb on - strand, within GFF296 at 13.108 kb on - strand, within GFF296 at 13.235 kb on + strand, within GFF296 at 13.235 kb on + strand, within GFF296 at 13.308 kb on + strand at 13.310 kb on + strand at 13.311 kb on - strand at 13.400 kb on - strand at 13.526 kb on + strand at 13.567 kb on + strand at 13.567 kb on + strand at 13.649 kb on + strand at 13.650 kb on - strand at 13.701 kb on - strand, within GFF297 at 13.905 kb on - strand, within GFF297 at 13.960 kb on + strand, within GFF297 at 13.960 kb on + strand, within GFF297 at 13.960 kb on + strand, within GFF297 at 13.961 kb on - strand, within GFF297 at 13.985 kb on - strand, within GFF297 at 14.045 kb on + strand, within GFF297 at 14.152 kb on + strand, within GFF297 at 14.153 kb on - strand, within GFF297 at 14.160 kb on + strand, within GFF297 at 14.211 kb on + strand, within GFF297 at 14.212 kb on - strand, within GFF297 at 14.212 kb on - strand, within GFF297 at 14.212 kb on - strand, within GFF297 at 14.212 kb on - strand, within GFF297 at 14.240 kb on + strand, within GFF297 at 14.382 kb on - strand at 14.460 kb on + strand at 14.674 kb on - strand at 14.746 kb on - strand at 14.864 kb on - strand at 14.864 kb on - strand at 15.026 kb on + strand at 15.134 kb on + strand at 15.231 kb on + strand at 15.234 kb on - strand at 15.299 kb on + strand at 15.300 kb on - strand at 15.427 kb on + strand
Per-strain Table
Position Strand Gene LocusTag Fraction LB_plus_SM_buffer with EIP16_phage 0.0875 MOI remove 12,663 + GFF296 0.39 -0.2 12,676 + GFF296 0.41 -0.2 12,676 + GFF296 0.41 +0.7 12,676 + GFF296 0.41 +0.1 12,677 - GFF296 0.41 +0.6 12,680 - GFF296 0.41 -1.1 12,755 + GFF296 0.47 -0.8 12,790 - GFF296 0.50 -1.9 12,791 + GFF296 0.50 +1.2 12,979 + GFF296 0.67 -0.2 12,979 + GFF296 0.67 -0.4 13,017 - GFF296 0.70 -0.5 13,108 - GFF296 0.78 -0.3 13,235 + GFF296 0.89 -0.0 13,235 + GFF296 0.89 +0.7 13,308 + +1.2 13,310 + -0.7 13,311 - -0.7 13,400 - -1.0 13,526 + +0.3 13,567 + -0.2 13,567 + -0.1 13,649 + -0.1 13,650 - -0.7 13,701 - GFF297 0.13 +0.0 13,905 - GFF297 0.36 -0.0 13,960 + GFF297 0.43 +0.4 13,960 + GFF297 0.43 -0.4 13,960 + GFF297 0.43 +1.6 13,961 - GFF297 0.43 +0.5 13,985 - GFF297 0.46 -0.2 14,045 + GFF297 0.53 +0.3 14,152 + GFF297 0.65 +1.8 14,153 - GFF297 0.65 -0.3 14,160 + GFF297 0.66 +0.5 14,211 + GFF297 0.72 +0.2 14,212 - GFF297 0.72 +0.1 14,212 - GFF297 0.72 +0.6 14,212 - GFF297 0.72 +1.2 14,212 - GFF297 0.72 +1.4 14,240 + GFF297 0.75 +0.2 14,382 - +0.0 14,460 + +0.5 14,674 - +1.6 14,746 - -0.4 14,864 - -0.1 14,864 - +0.1 15,026 + -0.8 15,134 + +0.2 15,231 + +0.0 15,234 - +0.6 15,299 + +0.4 15,300 - -0.4 15,427 + +0.6
Or see this region's nucleotide sequence