Experiment: LB_plus_SM_buffer with EIP16_phage 0.0875 MOI
Only strains with sufficient reads to estimate fitness are shown,
but the strain fitness values are still rather noisy and may be biased towards zero.
Strains near the edge of a gene are not shown as being associated with that
gene (they are in grey).
Strains in the central 10-90% of a gene are color coded by the insertion's strand.
Usually, "+" means that the selectable marker is encoded on the forward strand,
i.e., transcribed rightward.
500 nt GFF2960 and GFF2961 are separated by 82 nucleotides GFF2961 and GFF2962 overlap by 4 nucleotides GFF2962 and GFF2963 overlap by 4 nucleotides
GFF2960 - Cytosine deaminase (EC 3.5.4.1), at 44,554 to 45,834
GFF2960
GFF2961 - Putative sugar isomerase involved in processing of exogenous sialic acid, at 45,917 to 46,384
GFF2961
GFF2962 - N-acetylmannosamine kinase (EC 2.7.1.60), at 46,381 to 47,256
GFF2962
GFF2963 - N-acetylmannosamine-6-phosphate 2-epimerase (EC 5.1.3.9), at 47,253 to 47,942
GFF2963
Position (kb)
45
46
47 Strain fitness (log2 ratio)
-1
0
1 at 45.128 kb on - strand, within GFF2960 at 45.173 kb on - strand, within GFF2960 at 45.300 kb on + strand, within GFF2960 at 45.549 kb on + strand, within GFF2960 at 45.762 kb on - strand at 45.762 kb on - strand at 46.049 kb on - strand, within GFF2961 at 46.163 kb on + strand, within GFF2961 at 46.166 kb on + strand, within GFF2961 at 46.182 kb on + strand, within GFF2961 at 46.239 kb on - strand, within GFF2961 at 46.368 kb on - strand at 46.384 kb on + strand at 46.384 kb on + strand at 46.384 kb on + strand at 46.384 kb on + strand at 46.385 kb on - strand at 46.385 kb on - strand at 46.421 kb on - strand at 46.467 kb on - strand at 46.762 kb on + strand, within GFF2962 at 46.882 kb on + strand, within GFF2962 at 46.916 kb on + strand, within GFF2962 at 47.074 kb on + strand, within GFF2962 at 47.224 kb on - strand at 47.250 kb on + strand at 47.251 kb on - strand at 47.251 kb on - strand at 47.382 kb on - strand, within GFF2963 at 47.382 kb on - strand, within GFF2963 at 47.383 kb on + strand, within GFF2963
Per-strain Table
Position Strand Gene LocusTag Fraction LB_plus_SM_buffer with EIP16_phage 0.0875 MOI remove 45,128 - GFF2960 0.45 +0.3 45,173 - GFF2960 0.48 +0.0 45,300 + GFF2960 0.58 -0.7 45,549 + GFF2960 0.78 +1.5 45,762 - -0.3 45,762 - +0.5 46,049 - GFF2961 0.28 +0.4 46,163 + GFF2961 0.53 -0.3 46,166 + GFF2961 0.53 -0.3 46,182 + GFF2961 0.57 -0.1 46,239 - GFF2961 0.69 -0.3 46,368 - -0.4 46,384 + +0.0 46,384 + -0.1 46,384 + +0.1 46,384 + -0.2 46,385 - -0.0 46,385 - +0.5 46,421 - +1.5 46,467 - +0.4 46,762 + GFF2962 0.43 -0.2 46,882 + GFF2962 0.57 -0.1 46,916 + GFF2962 0.61 -0.3 47,074 + GFF2962 0.79 -0.1 47,224 - -0.6 47,250 + +0.6 47,251 - +0.1 47,251 - -0.6 47,382 - GFF2963 0.19 -0.3 47,382 - GFF2963 0.19 -0.0 47,383 + GFF2963 0.19 -0.3
Or see this region's nucleotide sequence