Strain Fitness in Salmonella enterica subsp. enterica serovar Typhimurium str. MS1868 around GFF2819

Experiment: LB_plus_SM_buffer with EIP16_phage 0.0875 MOI

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Only strains with sufficient reads to estimate fitness are shown, but the strain fitness values are still rather noisy and may be biased towards zero. Strains near the edge of a gene are not shown as being associated with that gene (they are in grey). Strains in the central 10-90% of a gene are color coded by the insertion's strand. Usually, "+" means that the selectable marker is encoded on the forward strand, i.e., transcribed rightward.

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500 ntGFF2817 and GFF2818 are separated by 3 nucleotidesGFF2818 and GFF2819 are separated by 121 nucleotidesGFF2819 and GFF2820 are separated by 84 nucleotides GFF2817 - Septum site-determining protein MinD, at 338,087 to 338,899 GFF2817 GFF2818 - Cell division topological specificity factor MinE, at 338,903 to 339,169 GFF2818 GFF2819 - Ribonuclease D (EC 3.1.26.3), at 339,291 to 340,406 GFF2819 GFF2820 - Long-chain-fatty-acid--CoA ligase (EC 6.2.1.3), at 340,491 to 342,176 GFF2820 Position (kb) 339 340 341Strain fitness (log2 ratio) -2 -1 0 1at 339.669 kb on - strand, within GFF2819at 339.947 kb on - strand, within GFF2819at 340.045 kb on + strand, within GFF2819at 340.046 kb on - strand, within GFF2819at 340.054 kb on - strand, within GFF2819at 340.156 kb on - strand, within GFF2819at 340.310 kb on - strandat 340.604 kb on + strandat 340.604 kb on + strandat 340.605 kb on - strandat 341.108 kb on - strand, within GFF2820at 341.256 kb on - strand, within GFF2820

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Per-strain Table

Position Strand Gene LocusTag Fraction LB_plus_SM_buffer with EIP16_phage 0.0875 MOI
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339,669 - GFF2819 0.34 -0.6
339,947 - GFF2819 0.59 -1.1
340,045 + GFF2819 0.68 -1.3
340,046 - GFF2819 0.68 -1.0
340,054 - GFF2819 0.68 -1.1
340,156 - GFF2819 0.78 -2.2
340,310 - -1.0
340,604 + +0.6
340,604 + +0.8
340,605 - +0.5
341,108 - GFF2820 0.37 -0.5
341,256 - GFF2820 0.45 -0.4

Or see this region's nucleotide sequence