Experiment: LB_plus_SM_buffer with EIP16_phage 0.0875 MOI
Only strains with sufficient reads to estimate fitness are shown,
but the strain fitness values are still rather noisy and may be biased towards zero.
Strains near the edge of a gene are not shown as being associated with that
gene (they are in grey).
Strains in the central 10-90% of a gene are color coded by the insertion's strand.
Usually, "+" means that the selectable marker is encoded on the forward strand,
i.e., transcribed rightward.
500 nt GFF2139 and GFF2140 are separated by 144 nucleotides GFF2140 and GFF2141 are separated by 122 nucleotides
GFF2139 - FIG005080: Possible exported protein, at 27,179 to 30,979
GFF2139
GFF2140 - TldD protein, part of TldE/TldD proteolytic complex, at 31,124 to 32,569
GFF2140
GFF2141 - LysR family transcriptional regulator QseA, at 32,692 to 33,603
GFF2141
Position (kb)
31
32
33 Strain fitness (log2 ratio)
-1
0
1
2 at 30.139 kb on + strand, within GFF2139 at 30.211 kb on + strand, within GFF2139 at 30.212 kb on - strand, within GFF2139 at 30.358 kb on + strand, within GFF2139 at 30.359 kb on - strand, within GFF2139 at 30.359 kb on - strand, within GFF2139 at 30.361 kb on + strand, within GFF2139 at 30.361 kb on + strand, within GFF2139 at 30.426 kb on + strand, within GFF2139 at 30.553 kb on - strand, within GFF2139 at 30.587 kb on - strand, within GFF2139 at 30.611 kb on - strand at 30.640 kb on + strand at 30.640 kb on + strand at 30.640 kb on + strand at 30.647 kb on - strand at 30.676 kb on - strand at 31.014 kb on - strand at 31.017 kb on + strand at 31.017 kb on + strand at 31.018 kb on - strand at 31.061 kb on - strand at 31.069 kb on - strand at 31.113 kb on + strand at 31.141 kb on - strand at 31.141 kb on - strand at 31.289 kb on - strand, within GFF2140 at 31.301 kb on + strand, within GFF2140 at 31.302 kb on - strand, within GFF2140 at 31.303 kb on + strand, within GFF2140 at 31.374 kb on + strand, within GFF2140 at 31.608 kb on + strand, within GFF2140 at 31.608 kb on + strand, within GFF2140 at 31.618 kb on - strand, within GFF2140 at 31.751 kb on + strand, within GFF2140 at 31.962 kb on + strand, within GFF2140 at 31.962 kb on + strand, within GFF2140 at 31.963 kb on - strand, within GFF2140 at 32.053 kb on + strand, within GFF2140 at 32.082 kb on - strand, within GFF2140 at 32.117 kb on + strand, within GFF2140 at 32.222 kb on + strand, within GFF2140 at 32.222 kb on + strand, within GFF2140 at 32.222 kb on + strand, within GFF2140 at 32.222 kb on + strand, within GFF2140 at 32.223 kb on - strand, within GFF2140 at 32.223 kb on - strand, within GFF2140 at 32.223 kb on - strand, within GFF2140 at 32.223 kb on - strand, within GFF2140 at 32.224 kb on + strand, within GFF2140 at 32.224 kb on + strand, within GFF2140 at 32.224 kb on + strand, within GFF2140 at 32.225 kb on - strand, within GFF2140 at 32.225 kb on - strand, within GFF2140 at 32.282 kb on + strand, within GFF2140 at 32.283 kb on - strand, within GFF2140 at 32.283 kb on - strand, within GFF2140 at 32.283 kb on - strand, within GFF2140 at 32.283 kb on - strand, within GFF2140 at 32.283 kb on - strand, within GFF2140 at 32.283 kb on - strand, within GFF2140 at 32.354 kb on + strand, within GFF2140 at 32.458 kb on + strand at 32.910 kb on - strand, within GFF2141 at 32.961 kb on + strand, within GFF2141 at 33.046 kb on - strand, within GFF2141 at 33.047 kb on + strand, within GFF2141 at 33.345 kb on + strand, within GFF2141 at 33.345 kb on + strand, within GFF2141 at 33.346 kb on - strand, within GFF2141 at 33.565 kb on - strand
Per-strain Table
Position Strand Gene LocusTag Fraction LB_plus_SM_buffer with EIP16_phage 0.0875 MOI remove 30,139 + GFF2139 0.78 +0.7 30,211 + GFF2139 0.80 -0.3 30,212 - GFF2139 0.80 -0.1 30,358 + GFF2139 0.84 -0.5 30,359 - GFF2139 0.84 +0.7 30,359 - GFF2139 0.84 -0.1 30,361 + GFF2139 0.84 -0.2 30,361 + GFF2139 0.84 -0.5 30,426 + GFF2139 0.85 +2.7 30,553 - GFF2139 0.89 +0.0 30,587 - GFF2139 0.90 +1.0 30,611 - +0.9 30,640 + -0.4 30,640 + -0.2 30,640 + -0.1 30,647 - +0.1 30,676 - -0.3 31,014 - -0.8 31,017 + -0.4 31,017 + +0.3 31,018 - +0.7 31,061 - -0.6 31,069 - +0.3 31,113 + -0.1 31,141 - -0.4 31,141 - +1.4 31,289 - GFF2140 0.11 -0.2 31,301 + GFF2140 0.12 +0.1 31,302 - GFF2140 0.12 +0.1 31,303 + GFF2140 0.12 -0.2 31,374 + GFF2140 0.17 -0.6 31,608 + GFF2140 0.33 +0.0 31,608 + GFF2140 0.33 -1.5 31,618 - GFF2140 0.34 +1.5 31,751 + GFF2140 0.43 -0.3 31,962 + GFF2140 0.58 -0.5 31,962 + GFF2140 0.58 -0.0 31,963 - GFF2140 0.58 +0.5 32,053 + GFF2140 0.64 -0.1 32,082 - GFF2140 0.66 -0.5 32,117 + GFF2140 0.69 -0.3 32,222 + GFF2140 0.76 -0.2 32,222 + GFF2140 0.76 +0.1 32,222 + GFF2140 0.76 -0.2 32,222 + GFF2140 0.76 +0.5 32,223 - GFF2140 0.76 -0.5 32,223 - GFF2140 0.76 -0.1 32,223 - GFF2140 0.76 -1.1 32,223 - GFF2140 0.76 +1.2 32,224 + GFF2140 0.76 -0.7 32,224 + GFF2140 0.76 +0.3 32,224 + GFF2140 0.76 +0.3 32,225 - GFF2140 0.76 -0.5 32,225 - GFF2140 0.76 -0.8 32,282 + GFF2140 0.80 -1.3 32,283 - GFF2140 0.80 -0.1 32,283 - GFF2140 0.80 +0.4 32,283 - GFF2140 0.80 -0.4 32,283 - GFF2140 0.80 +1.3 32,283 - GFF2140 0.80 -0.2 32,283 - GFF2140 0.80 -0.6 32,354 + GFF2140 0.85 +0.5 32,458 + +1.0 32,910 - GFF2141 0.24 +1.3 32,961 + GFF2141 0.29 -0.6 33,046 - GFF2141 0.39 +0.5 33,047 + GFF2141 0.39 -0.1 33,345 + GFF2141 0.72 -0.5 33,345 + GFF2141 0.72 -0.7 33,346 - GFF2141 0.72 -0.6 33,565 - -0.3
Or see this region's nucleotide sequence