Experiment: LB_plus_SM_buffer with EIP16_phage 0.0875 MOI
Only strains with sufficient reads to estimate fitness are shown,
but the strain fitness values are still rather noisy and may be biased towards zero.
Strains near the edge of a gene are not shown as being associated with that
gene (they are in grey).
Strains in the central 10-90% of a gene are color coded by the insertion's strand.
Usually, "+" means that the selectable marker is encoded on the forward strand,
i.e., transcribed rightward.
500 nt GFF1955 and GFF1956 are separated by 177 nucleotides GFF1956 and GFF1957 are separated by 370 nucleotides GFF1957 and GFF1958 are separated by 60 nucleotides
GFF1955 - 3-ketoacyl-CoA thiolase (EC 2.3.1.16) @ Acetyl-CoA acetyltransferase (EC 2.3.1.9), at 146,642 to 147,952
GFF1955
GFF1956 - FIG00637865: hypothetical protein, at 148,130 to 148,414
GFF1956
GFF1957 - Long-chain fatty acid transport protein, at 148,785 to 150,092
GFF1957
GFF1958 - Lipoprotein, at 150,153 to 150,908
GFF1958
Position (kb)
148
149
150
151 Strain fitness (log2 ratio)
-2
-1
0
1 at 147.904 kb on + strand at 147.905 kb on - strand at 147.905 kb on - strand at 147.956 kb on - strand at 148.053 kb on - strand at 148.417 kb on + strand at 148.639 kb on + strand at 148.765 kb on + strand at 148.966 kb on + strand, within GFF1957 at 148.967 kb on - strand, within GFF1957 at 149.013 kb on - strand, within GFF1957 at 149.063 kb on - strand, within GFF1957 at 149.150 kb on + strand, within GFF1957 at 149.150 kb on + strand, within GFF1957 at 149.150 kb on + strand, within GFF1957 at 149.151 kb on - strand, within GFF1957 at 149.160 kb on - strand, within GFF1957 at 149.160 kb on - strand, within GFF1957 at 149.163 kb on + strand, within GFF1957 at 149.164 kb on - strand, within GFF1957 at 149.248 kb on + strand, within GFF1957 at 149.266 kb on + strand, within GFF1957 at 149.267 kb on - strand, within GFF1957 at 149.541 kb on + strand, within GFF1957 at 149.541 kb on + strand, within GFF1957 at 149.564 kb on + strand, within GFF1957 at 149.666 kb on + strand, within GFF1957 at 149.700 kb on - strand, within GFF1957 at 149.823 kb on + strand, within GFF1957 at 149.823 kb on + strand, within GFF1957 at 149.823 kb on + strand, within GFF1957 at 149.957 kb on + strand, within GFF1957 at 149.991 kb on + strand at 149.991 kb on + strand at 149.992 kb on - strand at 149.993 kb on + strand at 149.993 kb on + strand at 149.993 kb on + strand at 149.994 kb on - strand at 149.994 kb on - strand at 150.259 kb on + strand, within GFF1958 at 150.278 kb on + strand, within GFF1958 at 150.279 kb on - strand, within GFF1958 at 150.279 kb on - strand, within GFF1958 at 150.279 kb on - strand, within GFF1958 at 150.281 kb on - strand, within GFF1958 at 150.467 kb on + strand, within GFF1958 at 150.468 kb on - strand, within GFF1958 at 150.469 kb on + strand, within GFF1958 at 150.469 kb on + strand, within GFF1958 at 150.470 kb on - strand, within GFF1958 at 150.540 kb on - strand, within GFF1958 at 150.586 kb on - strand, within GFF1958 at 150.586 kb on - strand, within GFF1958 at 150.591 kb on - strand, within GFF1958 at 150.725 kb on - strand, within GFF1958 at 150.725 kb on - strand, within GFF1958 at 150.761 kb on - strand, within GFF1958 at 150.761 kb on - strand, within GFF1958 at 150.790 kb on + strand, within GFF1958 at 150.791 kb on - strand, within GFF1958 at 150.908 kb on + strand at 150.928 kb on - strand at 150.928 kb on - strand at 150.928 kb on - strand at 150.928 kb on - strand at 150.928 kb on - strand at 151.018 kb on + strand at 151.018 kb on + strand at 151.019 kb on - strand at 151.019 kb on - strand at 151.019 kb on - strand at 151.019 kb on - strand at 151.077 kb on - strand
Per-strain Table
Position Strand Gene LocusTag Fraction LB_plus_SM_buffer with EIP16_phage 0.0875 MOI remove 147,904 + +0.0 147,905 - -0.2 147,905 - -0.1 147,956 - +0.4 148,053 - +0.4 148,417 + +0.9 148,639 + -0.4 148,765 + -0.2 148,966 + GFF1957 0.14 -0.9 148,967 - GFF1957 0.14 +1.1 149,013 - GFF1957 0.17 -0.1 149,063 - GFF1957 0.21 -1.3 149,150 + GFF1957 0.28 -0.2 149,150 + GFF1957 0.28 -0.7 149,150 + GFF1957 0.28 -0.8 149,151 - GFF1957 0.28 -0.6 149,160 - GFF1957 0.29 -0.7 149,160 - GFF1957 0.29 -2.3 149,163 + GFF1957 0.29 -0.4 149,164 - GFF1957 0.29 -0.4 149,248 + GFF1957 0.35 -1.7 149,266 + GFF1957 0.37 -0.6 149,267 - GFF1957 0.37 +0.9 149,541 + GFF1957 0.58 -0.6 149,541 + GFF1957 0.58 -0.6 149,564 + GFF1957 0.60 -1.7 149,666 + GFF1957 0.67 -1.6 149,700 - GFF1957 0.70 +0.1 149,823 + GFF1957 0.79 -0.6 149,823 + GFF1957 0.79 -0.1 149,823 + GFF1957 0.79 -0.1 149,957 + GFF1957 0.90 -0.8 149,991 + -0.5 149,991 + -0.7 149,992 - -0.3 149,993 + -0.6 149,993 + +0.1 149,993 + +0.3 149,994 - -0.8 149,994 - +0.2 150,259 + GFF1958 0.14 -0.2 150,278 + GFF1958 0.17 -0.2 150,279 - GFF1958 0.17 -0.0 150,279 - GFF1958 0.17 -0.2 150,279 - GFF1958 0.17 -0.2 150,281 - GFF1958 0.17 -0.2 150,467 + GFF1958 0.42 +0.5 150,468 - GFF1958 0.42 -0.2 150,469 + GFF1958 0.42 -0.3 150,469 + GFF1958 0.42 -0.1 150,470 - GFF1958 0.42 -0.1 150,540 - GFF1958 0.51 +0.3 150,586 - GFF1958 0.57 -1.2 150,586 - GFF1958 0.57 -0.7 150,591 - GFF1958 0.58 -0.2 150,725 - GFF1958 0.76 -0.7 150,725 - GFF1958 0.76 +0.2 150,761 - GFF1958 0.80 -1.4 150,761 - GFF1958 0.80 -0.0 150,790 + GFF1958 0.84 +0.6 150,791 - GFF1958 0.84 -0.4 150,908 + -0.6 150,928 - +0.0 150,928 - -0.9 150,928 - -0.3 150,928 - -0.7 150,928 - -0.6 151,018 + -0.5 151,018 + -0.5 151,019 - +0.1 151,019 - +0.2 151,019 - +0.2 151,019 - -0.2 151,077 - -0.8
Or see this region's nucleotide sequence