Experiment: LB_plus_SM_buffer with EIP16_phage 0.0875 MOI
Only strains with sufficient reads to estimate fitness are shown,
but the strain fitness values are still rather noisy and may be biased towards zero.
Strains near the edge of a gene are not shown as being associated with that
gene (they are in grey).
Strains in the central 10-90% of a gene are color coded by the insertion's strand.
Usually, "+" means that the selectable marker is encoded on the forward strand,
i.e., transcribed rightward.
500 nt GFF1922 and GFF1923 are separated by 63 nucleotides GFF1923 and GFF1924 are separated by 1 nucleotides
GFF1922 - Lysine-specific permease, at 113,568 to 114,974
GFF1922
GFF1923 - FIG074102: hypothetical protein, at 115,038 to 116,141
GFF1923
GFF1924 - Putative amino acid transporter, at 116,143 to 117,564
GFF1924
Position (kb)
115
116
117 Strain fitness (log2 ratio)
-1
0
1
2 at 114.341 kb on - strand, within GFF1922 at 114.493 kb on + strand, within GFF1922 at 114.567 kb on + strand, within GFF1922 at 114.569 kb on + strand, within GFF1922 at 114.569 kb on + strand, within GFF1922 at 114.569 kb on + strand, within GFF1922 at 114.570 kb on - strand, within GFF1922 at 114.570 kb on - strand, within GFF1922 at 114.570 kb on - strand, within GFF1922 at 114.571 kb on + strand, within GFF1922 at 114.572 kb on - strand, within GFF1922 at 114.606 kb on - strand, within GFF1922 at 114.606 kb on - strand, within GFF1922 at 114.677 kb on - strand, within GFF1922 at 114.677 kb on - strand, within GFF1922 at 114.783 kb on - strand, within GFF1922 at 114.803 kb on - strand, within GFF1922 at 114.803 kb on - strand, within GFF1922 at 114.810 kb on + strand, within GFF1922 at 114.858 kb on - strand at 114.861 kb on + strand at 115.019 kb on + strand at 115.095 kb on - strand at 115.097 kb on - strand at 115.167 kb on - strand, within GFF1923 at 115.191 kb on - strand, within GFF1923 at 115.293 kb on - strand, within GFF1923 at 115.378 kb on + strand, within GFF1923 at 115.378 kb on + strand, within GFF1923 at 115.378 kb on + strand, within GFF1923 at 115.378 kb on + strand, within GFF1923 at 115.379 kb on - strand, within GFF1923 at 115.379 kb on - strand, within GFF1923 at 115.379 kb on - strand, within GFF1923 at 115.379 kb on - strand, within GFF1923 at 115.382 kb on + strand, within GFF1923 at 115.531 kb on + strand, within GFF1923 at 115.700 kb on + strand, within GFF1923 at 115.749 kb on - strand, within GFF1923 at 115.859 kb on + strand, within GFF1923 at 115.912 kb on - strand, within GFF1923 at 115.925 kb on + strand, within GFF1923 at 115.926 kb on - strand, within GFF1923 at 116.137 kb on + strand at 116.144 kb on + strand at 116.175 kb on + strand at 116.404 kb on - strand, within GFF1924 at 116.451 kb on - strand, within GFF1924 at 116.548 kb on + strand, within GFF1924 at 116.549 kb on - strand, within GFF1924 at 116.643 kb on + strand, within GFF1924 at 116.644 kb on - strand, within GFF1924 at 116.646 kb on - strand, within GFF1924 at 116.646 kb on - strand, within GFF1924 at 116.697 kb on + strand, within GFF1924 at 116.698 kb on - strand, within GFF1924 at 116.710 kb on - strand, within GFF1924 at 116.757 kb on + strand, within GFF1924 at 116.757 kb on + strand, within GFF1924 at 116.758 kb on - strand, within GFF1924 at 116.758 kb on - strand, within GFF1924 at 116.835 kb on - strand, within GFF1924 at 116.835 kb on - strand, within GFF1924 at 116.835 kb on - strand, within GFF1924 at 116.835 kb on - strand, within GFF1924 at 116.835 kb on - strand, within GFF1924 at 116.835 kb on - strand, within GFF1924 at 116.835 kb on - strand, within GFF1924 at 117.013 kb on - strand, within GFF1924 at 117.018 kb on - strand, within GFF1924 at 117.088 kb on - strand, within GFF1924 at 117.088 kb on - strand, within GFF1924
Per-strain Table
Position Strand Gene LocusTag Fraction LB_plus_SM_buffer with EIP16_phage 0.0875 MOI remove 114,341 - GFF1922 0.55 -0.2 114,493 + GFF1922 0.66 +0.8 114,567 + GFF1922 0.71 +0.3 114,569 + GFF1922 0.71 +0.1 114,569 + GFF1922 0.71 -0.1 114,569 + GFF1922 0.71 +0.3 114,570 - GFF1922 0.71 +0.7 114,570 - GFF1922 0.71 -0.6 114,570 - GFF1922 0.71 +0.4 114,571 + GFF1922 0.71 +0.3 114,572 - GFF1922 0.71 +0.0 114,606 - GFF1922 0.74 +0.7 114,606 - GFF1922 0.74 +0.4 114,677 - GFF1922 0.79 +0.7 114,677 - GFF1922 0.79 +0.2 114,783 - GFF1922 0.86 +0.4 114,803 - GFF1922 0.88 +0.5 114,803 - GFF1922 0.88 +0.1 114,810 + GFF1922 0.88 -0.4 114,858 - +0.4 114,861 + +0.4 115,019 + -0.2 115,095 - -0.1 115,097 - -0.3 115,167 - GFF1923 0.12 -0.1 115,191 - GFF1923 0.14 +0.1 115,293 - GFF1923 0.23 +0.9 115,378 + GFF1923 0.31 +0.9 115,378 + GFF1923 0.31 +1.4 115,378 + GFF1923 0.31 +0.7 115,378 + GFF1923 0.31 -0.1 115,379 - GFF1923 0.31 +0.3 115,379 - GFF1923 0.31 +2.5 115,379 - GFF1923 0.31 -0.0 115,379 - GFF1923 0.31 -0.1 115,382 + GFF1923 0.31 +0.5 115,531 + GFF1923 0.45 -0.0 115,700 + GFF1923 0.60 +0.7 115,749 - GFF1923 0.64 +0.1 115,859 + GFF1923 0.74 -0.2 115,912 - GFF1923 0.79 -0.2 115,925 + GFF1923 0.80 -0.0 115,926 - GFF1923 0.80 +0.3 116,137 + +0.5 116,144 + -0.3 116,175 + -0.0 116,404 - GFF1924 0.18 -0.7 116,451 - GFF1924 0.22 +0.2 116,548 + GFF1924 0.28 -0.1 116,549 - GFF1924 0.29 +0.3 116,643 + GFF1924 0.35 -0.1 116,644 - GFF1924 0.35 +0.9 116,646 - GFF1924 0.35 +0.1 116,646 - GFF1924 0.35 -0.3 116,697 + GFF1924 0.39 +1.7 116,698 - GFF1924 0.39 -0.8 116,710 - GFF1924 0.40 -0.3 116,757 + GFF1924 0.43 +0.2 116,757 + GFF1924 0.43 +0.3 116,758 - GFF1924 0.43 +1.2 116,758 - GFF1924 0.43 -0.5 116,835 - GFF1924 0.49 -0.0 116,835 - GFF1924 0.49 +0.6 116,835 - GFF1924 0.49 -0.6 116,835 - GFF1924 0.49 +1.1 116,835 - GFF1924 0.49 -0.1 116,835 - GFF1924 0.49 -0.1 116,835 - GFF1924 0.49 +0.0 117,013 - GFF1924 0.61 +0.4 117,018 - GFF1924 0.62 +0.4 117,088 - GFF1924 0.66 -0.1 117,088 - GFF1924 0.66 -1.2
Or see this region's nucleotide sequence