Experiment: LB_plus_SM_buffer with EIP16_phage 0.0875 MOI
Only strains with sufficient reads to estimate fitness are shown,
but the strain fitness values are still rather noisy and may be biased towards zero.
Strains near the edge of a gene are not shown as being associated with that
gene (they are in grey).
Strains in the central 10-90% of a gene are color coded by the insertion's strand.
Usually, "+" means that the selectable marker is encoded on the forward strand,
i.e., transcribed rightward.
500 nt GFF1906 and GFF1907 are separated by 80 nucleotides GFF1907 and GFF1908 overlap by 2 nucleotides GFF1908 and GFF1909 are separated by 80 nucleotides GFF1909 and GFF1910 are separated by 5 nucleotides GFF1910 and GFF1911 are separated by 56 nucleotides
GFF1906 - Putative sugar phosphotransferase component II B, at 102,724 to 102,996
GFF1906
GFF1907 - Ascorbate-specific PTS system, EIIA component (EC 2.7.1.-), at 103,077 to 103,520
GFF1907
GFF1908 - FIG01046667: hypothetical protein, at 103,519 to 103,692
GFF1908
GFF1909 - Transcriptional regulator, LacI family, at 103,773 to 104,792
GFF1909
GFF1910 - Putative Nudix hydrolase YfcD (EC 3.6.-.-), at 104,798 to 105,352
GFF1910
GFF1911 - FIG002292: Phosphodiesterase yfcE (EC 3.1.4.-), at 105,409 to 105,960
GFF1911
Position (kb) 103
104
105 Strain fitness (log2 ratio) -1
0
1 at 103.355 kb on - strand, within GFF1907 at 103.482 kb on + strand at 103.482 kb on + strand at 103.524 kb on - strand at 103.580 kb on + strand, within GFF1908 at 103.580 kb on + strand, within GFF1908 at 103.700 kb on - strand at 103.939 kb on - strand, within GFF1909 at 103.940 kb on + strand, within GFF1909 at 103.940 kb on + strand, within GFF1909 at 103.941 kb on - strand, within GFF1909 at 104.081 kb on + strand, within GFF1909 at 104.265 kb on - strand, within GFF1909 at 104.276 kb on + strand, within GFF1909 at 104.299 kb on - strand, within GFF1909 at 104.423 kb on + strand, within GFF1909 at 104.423 kb on + strand, within GFF1909 at 104.423 kb on + strand, within GFF1909 at 104.424 kb on - strand, within GFF1909 at 104.424 kb on - strand, within GFF1909 at 104.444 kb on + strand, within GFF1909 at 104.531 kb on - strand, within GFF1909 at 104.563 kb on + strand, within GFF1909 at 104.770 kb on + strand at 104.770 kb on + strand at 104.770 kb on + strand at 104.770 kb on + strand at 104.771 kb on - strand at 105.067 kb on + strand, within GFF1910 at 105.149 kb on + strand, within GFF1910 at 105.150 kb on - strand, within GFF1910 at 105.150 kb on - strand, within GFF1910 at 105.154 kb on + strand, within GFF1910 at 105.228 kb on + strand, within GFF1910 at 105.229 kb on - strand, within GFF1910 at 105.325 kb on + strand at 105.367 kb on - strand at 105.381 kb on + strand at 105.417 kb on + strand at 105.417 kb on + strand at 105.545 kb on + strand, within GFF1911 at 105.582 kb on + strand, within GFF1911 at 105.582 kb on + strand, within GFF1911
Per-strain Table
Position Strand Gene LocusTag Fraction LB_plus_SM_buffer with EIP16_phage 0.0875 MOI remove 103,355 - GFF1907 0.63 -0.7 103,482 + -0.6 103,482 + +0.2 103,524 - +0.2 103,580 + GFF1908 0.35 -0.2 103,580 + GFF1908 0.35 +0.7 103,700 - +0.1 103,939 - GFF1909 0.16 +0.2 103,940 + GFF1909 0.16 -0.0 103,940 + GFF1909 0.16 +1.0 103,941 - GFF1909 0.16 +1.3 104,081 + GFF1909 0.30 -0.4 104,265 - GFF1909 0.48 -0.2 104,276 + GFF1909 0.49 -0.4 104,299 - GFF1909 0.52 +0.4 104,423 + GFF1909 0.64 +0.5 104,423 + GFF1909 0.64 -0.1 104,423 + GFF1909 0.64 +0.1 104,424 - GFF1909 0.64 +1.5 104,424 - GFF1909 0.64 +0.7 104,444 + GFF1909 0.66 +0.5 104,531 - GFF1909 0.74 +0.6 104,563 + GFF1909 0.77 -0.0 104,770 + -0.1 104,770 + -0.3 104,770 + -0.2 104,770 + +0.3 104,771 - +0.6 105,067 + GFF1910 0.48 +0.1 105,149 + GFF1910 0.63 -0.2 105,150 - GFF1910 0.63 -0.4 105,150 - GFF1910 0.63 +0.7 105,154 + GFF1910 0.64 -1.0 105,228 + GFF1910 0.77 +0.4 105,229 - GFF1910 0.78 +0.6 105,325 + -0.2 105,367 - +0.9 105,381 + -0.2 105,417 + -0.5 105,417 + -0.9 105,545 + GFF1911 0.25 +0.0 105,582 + GFF1911 0.31 +0.9 105,582 + GFF1911 0.31 -0.2
Or see this region's nucleotide sequence