Experiment: LB_plus_SM_buffer with EIP16_phage 0.0875 MOI
Only strains with sufficient reads to estimate fitness are shown,
but the strain fitness values are still rather noisy and may be biased towards zero.
Strains near the edge of a gene are not shown as being associated with that
gene (they are in grey).
Strains in the central 10-90% of a gene are color coded by the insertion's strand.
Usually, "+" means that the selectable marker is encoded on the forward strand,
i.e., transcribed rightward.
500 nt GFF1741 and GFF1742 overlap by 1 nucleotides GFF1742 and GFF1743 are separated by 157 nucleotides GFF1743 and GFF1744 overlap by 32 nucleotides
GFF1741 - FIG00553873: hypothetical protein, at 42,150 to 42,734
GFF1741
GFF1742 - FIG004212: hypothetical protein, at 42,734 to 43,489
GFF1742
GFF1743 - Putative LysR-family transcriptional regulator YidZ, at 43,647 to 44,606
GFF1743
GFF1744 - Putative transport protein, at 44,575 to 45,762
GFF1744
Position (kb) 43
44
45 Strain fitness (log2 ratio) -1
0
1 at 42.693 kb on - strand at 42.716 kb on + strand at 42.717 kb on - strand at 42.717 kb on - strand at 42.717 kb on - strand at 42.736 kb on - strand at 42.754 kb on - strand at 42.908 kb on + strand, within GFF1742 at 43.035 kb on - strand, within GFF1742 at 43.103 kb on + strand, within GFF1742 at 43.176 kb on - strand, within GFF1742 at 43.239 kb on + strand, within GFF1742 at 43.240 kb on - strand, within GFF1742 at 43.240 kb on - strand, within GFF1742 at 43.330 kb on - strand, within GFF1742 at 43.330 kb on - strand, within GFF1742 at 43.336 kb on + strand, within GFF1742 at 43.459 kb on + strand at 43.460 kb on - strand at 43.460 kb on - strand at 43.531 kb on - strand at 43.615 kb on + strand at 43.686 kb on + strand at 43.687 kb on - strand at 43.706 kb on + strand at 43.715 kb on + strand at 43.716 kb on - strand at 43.728 kb on + strand at 43.731 kb on + strand at 43.731 kb on + strand at 43.832 kb on - strand, within GFF1743 at 43.964 kb on + strand, within GFF1743 at 43.991 kb on + strand, within GFF1743 at 43.991 kb on + strand, within GFF1743 at 44.070 kb on + strand, within GFF1743 at 44.086 kb on - strand, within GFF1743 at 44.276 kb on + strand, within GFF1743 at 44.277 kb on - strand, within GFF1743 at 44.397 kb on - strand, within GFF1743 at 44.506 kb on - strand, within GFF1743 at 44.553 kb on + strand at 44.553 kb on + strand at 44.560 kb on - strand at 44.560 kb on - strand at 44.565 kb on + strand at 44.576 kb on + strand at 44.659 kb on + strand at 44.664 kb on + strand at 44.665 kb on - strand at 44.665 kb on - strand at 44.691 kb on + strand at 44.691 kb on + strand at 44.856 kb on + strand, within GFF1744 at 44.948 kb on - strand, within GFF1744 at 44.948 kb on - strand, within GFF1744 at 45.191 kb on - strand, within GFF1744 at 45.258 kb on + strand, within GFF1744 at 45.279 kb on + strand, within GFF1744 at 45.281 kb on + strand, within GFF1744 at 45.313 kb on - strand, within GFF1744 at 45.357 kb on + strand, within GFF1744 at 45.437 kb on + strand, within GFF1744 at 45.438 kb on - strand, within GFF1744 at 45.528 kb on + strand, within GFF1744
Per-strain Table
Position Strand Gene LocusTag Fraction LB_plus_SM_buffer with EIP16_phage 0.0875 MOI remove 42,693 - +0.4 42,716 + +0.6 42,717 - +0.5 42,717 - -0.1 42,717 - +0.1 42,736 - -0.4 42,754 - +0.1 42,908 + GFF1742 0.23 +0.1 43,035 - GFF1742 0.40 +0.1 43,103 + GFF1742 0.49 +0.4 43,176 - GFF1742 0.58 +0.2 43,239 + GFF1742 0.67 +0.3 43,240 - GFF1742 0.67 +0.4 43,240 - GFF1742 0.67 +0.9 43,330 - GFF1742 0.79 -0.2 43,330 - GFF1742 0.79 +0.3 43,336 + GFF1742 0.80 -1.0 43,459 + +0.1 43,460 - +0.4 43,460 - -0.0 43,531 - +0.7 43,615 + -0.1 43,686 + +0.5 43,687 - -0.7 43,706 + -0.1 43,715 + -0.5 43,716 - +0.2 43,728 + -0.1 43,731 + +1.0 43,731 + +0.2 43,832 - GFF1743 0.19 +0.2 43,964 + GFF1743 0.33 -0.0 43,991 + GFF1743 0.36 +1.4 43,991 + GFF1743 0.36 -0.6 44,070 + GFF1743 0.44 +1.4 44,086 - GFF1743 0.46 -0.5 44,276 + GFF1743 0.66 -0.1 44,277 - GFF1743 0.66 +0.6 44,397 - GFF1743 0.78 +0.1 44,506 - GFF1743 0.89 +0.7 44,553 + +0.3 44,553 + -0.2 44,560 - +0.1 44,560 - +0.0 44,565 + -0.4 44,576 + -0.1 44,659 + +1.1 44,664 + -0.3 44,665 - -0.3 44,665 - -0.2 44,691 + +0.5 44,691 + +0.0 44,856 + GFF1744 0.24 -0.9 44,948 - GFF1744 0.31 +1.2 44,948 - GFF1744 0.31 -0.4 45,191 - GFF1744 0.52 +0.5 45,258 + GFF1744 0.57 +0.9 45,279 + GFF1744 0.59 +0.2 45,281 + GFF1744 0.59 -0.1 45,313 - GFF1744 0.62 +0.5 45,357 + GFF1744 0.66 +0.3 45,437 + GFF1744 0.73 +0.2 45,438 - GFF1744 0.73 -0.1 45,528 + GFF1744 0.80 +0.1
Or see this region's nucleotide sequence