Experiment: LB_plus_SM_buffer with EIP16_phage 0.0875 MOI
Only strains with sufficient reads to estimate fitness are shown,
but the strain fitness values are still rather noisy and may be biased towards zero.
Strains near the edge of a gene are not shown as being associated with that
gene (they are in grey).
Strains in the central 10-90% of a gene are color coded by the insertion's strand.
Usually, "+" means that the selectable marker is encoded on the forward strand,
i.e., transcribed rightward.
500 nt GFF1704 and GFF1705 are separated by 12 nucleotides GFF1705 and GFF1706 are separated by 133 nucleotides
GFF1703 - Permeases of the major facilitator superfamily, at 904 to 2,331
GFF1703
GFF1704 - Ribose operon repressor, at 2,297 to 3,286
GFF1704
GFF1705 - Ribokinase (EC 2.7.1.15), at 3,299 to 4,228
GFF1705
GFF1706 - Ribose ABC transport system, periplasmic ribose-binding protein RbsB (TC 3.A.1.2.1), at 4,362 to 5,252
GFF1706
Position (kb)
3
4
5 Strain fitness (log2 ratio)
-2
-1
0
1
2 at 2.484 kb on - strand, within GFF1704 at 2.520 kb on - strand, within GFF1704 at 2.650 kb on - strand, within GFF1704 at 2.650 kb on - strand, within GFF1704 at 2.744 kb on - strand, within GFF1704 at 2.744 kb on - strand, within GFF1704 at 2.778 kb on + strand, within GFF1704 at 2.812 kb on + strand, within GFF1704 at 2.813 kb on - strand, within GFF1704 at 2.878 kb on + strand, within GFF1704 at 3.025 kb on + strand, within GFF1704 at 3.075 kb on + strand, within GFF1704 at 3.102 kb on - strand, within GFF1704 at 3.102 kb on - strand, within GFF1704 at 3.102 kb on - strand, within GFF1704 at 3.102 kb on - strand, within GFF1704 at 3.128 kb on + strand, within GFF1704 at 3.162 kb on + strand, within GFF1704 at 3.286 kb on + strand at 3.286 kb on + strand at 3.287 kb on - strand at 3.289 kb on + strand at 3.463 kb on + strand, within GFF1705 at 3.530 kb on + strand, within GFF1705 at 3.563 kb on - strand, within GFF1705 at 3.745 kb on + strand, within GFF1705 at 3.746 kb on - strand, within GFF1705 at 3.746 kb on - strand, within GFF1705 at 3.746 kb on - strand, within GFF1705 at 3.748 kb on + strand, within GFF1705 at 3.748 kb on + strand, within GFF1705 at 3.749 kb on - strand, within GFF1705 at 3.749 kb on - strand, within GFF1705 at 3.959 kb on - strand, within GFF1705 at 3.959 kb on - strand, within GFF1705 at 3.967 kb on + strand, within GFF1705 at 3.968 kb on - strand, within GFF1705 at 3.968 kb on - strand, within GFF1705 at 4.006 kb on + strand, within GFF1705 at 4.013 kb on - strand, within GFF1705 at 4.058 kb on - strand, within GFF1705 at 4.070 kb on - strand, within GFF1705 at 4.070 kb on - strand, within GFF1705 at 4.172 kb on - strand at 4.177 kb on + strand at 4.321 kb on - strand at 4.321 kb on - strand at 4.342 kb on + strand at 4.361 kb on - strand at 4.361 kb on - strand at 4.563 kb on - strand, within GFF1706 at 4.677 kb on - strand, within GFF1706 at 4.715 kb on + strand, within GFF1706 at 4.715 kb on + strand, within GFF1706 at 5.122 kb on - strand, within GFF1706 at 5.141 kb on + strand, within GFF1706 at 5.150 kb on + strand, within GFF1706
Per-strain Table
Position Strand Gene LocusTag Fraction LB_plus_SM_buffer with EIP16_phage 0.0875 MOI remove 2,484 - GFF1704 0.19 -0.9 2,520 - GFF1704 0.23 -0.1 2,650 - GFF1704 0.36 -1.1 2,650 - GFF1704 0.36 -0.1 2,744 - GFF1704 0.45 -0.9 2,744 - GFF1704 0.45 -0.3 2,778 + GFF1704 0.49 -0.2 2,812 + GFF1704 0.52 +1.7 2,813 - GFF1704 0.52 -0.4 2,878 + GFF1704 0.59 +0.2 3,025 + GFF1704 0.74 -0.1 3,075 + GFF1704 0.79 +0.3 3,102 - GFF1704 0.81 +0.1 3,102 - GFF1704 0.81 -0.6 3,102 - GFF1704 0.81 +0.3 3,102 - GFF1704 0.81 +0.3 3,128 + GFF1704 0.84 +0.3 3,162 + GFF1704 0.87 -0.2 3,286 + -0.7 3,286 + -1.0 3,287 - +0.2 3,289 + -2.1 3,463 + GFF1705 0.18 -0.3 3,530 + GFF1705 0.25 -0.1 3,563 - GFF1705 0.28 -0.3 3,745 + GFF1705 0.48 +0.1 3,746 - GFF1705 0.48 +0.1 3,746 - GFF1705 0.48 +0.1 3,746 - GFF1705 0.48 -1.1 3,748 + GFF1705 0.48 -0.3 3,748 + GFF1705 0.48 +0.9 3,749 - GFF1705 0.48 +0.3 3,749 - GFF1705 0.48 -0.9 3,959 - GFF1705 0.71 -0.2 3,959 - GFF1705 0.71 +0.8 3,967 + GFF1705 0.72 -1.5 3,968 - GFF1705 0.72 -0.7 3,968 - GFF1705 0.72 +0.3 4,006 + GFF1705 0.76 -0.3 4,013 - GFF1705 0.77 -0.0 4,058 - GFF1705 0.82 +0.2 4,070 - GFF1705 0.83 +0.7 4,070 - GFF1705 0.83 +1.6 4,172 - +0.0 4,177 + -0.4 4,321 - +0.2 4,321 - -0.1 4,342 + +0.0 4,361 - -0.2 4,361 - -0.5 4,563 - GFF1706 0.23 +0.8 4,677 - GFF1706 0.35 +1.8 4,715 + GFF1706 0.40 -0.3 4,715 + GFF1706 0.40 +0.5 5,122 - GFF1706 0.85 +0.3 5,141 + GFF1706 0.87 +1.4 5,150 + GFF1706 0.88 +1.5
Or see this region's nucleotide sequence