Experiment: LB_plus_SM_buffer with EIP16_phage 0.0875 MOI
Only strains with sufficient reads to estimate fitness are shown,
but the strain fitness values are still rather noisy and may be biased towards zero.
Strains near the edge of a gene are not shown as being associated with that
gene (they are in grey).
Strains in the central 10-90% of a gene are color coded by the insertion's strand.
Usually, "+" means that the selectable marker is encoded on the forward strand,
i.e., transcribed rightward.
500 nt GFF1648 and GFF1649 overlap by 4 nucleotides GFF1649 and GFF1650 are separated by 52 nucleotides GFF1650 and GFF1651 are separated by 40 nucleotides GFF1651 and GFF1652 overlap by 4 nucleotides
GFF1648 - Nicotinamidase/isochorismatase family protein, at 33,629 to 34,195
GFF1648
GFF1649 - FIG003003: hypothetical protein, at 34,192 to 35,010
GFF1649
GFF1650 - FIG01046209: inner membrane protein, at 35,063 to 35,458
GFF1650
GFF1651 - tRNA (uridine-5-oxyacetic acid methyl ester) 34 synthase, at 35,499 to 36,242
GFF1651
GFF1652 - tRNA (5-methoxyuridine) 34 synthase, at 36,239 to 37,210
GFF1652
Position (kb) 35
36 Strain fitness (log2 ratio) -1
0
1 at 34.138 kb on + strand, within GFF1648 at 34.199 kb on - strand at 34.566 kb on + strand, within GFF1649 at 34.567 kb on - strand, within GFF1649 at 34.578 kb on - strand, within GFF1649 at 34.641 kb on - strand, within GFF1649 at 34.685 kb on + strand, within GFF1649 at 34.686 kb on - strand, within GFF1649 at 35.026 kb on + strand at 35.027 kb on - strand at 35.078 kb on + strand at 35.144 kb on + strand, within GFF1650 at 35.288 kb on - strand, within GFF1650 at 35.424 kb on - strand at 35.424 kb on - strand at 35.424 kb on - strand at 35.426 kb on - strand at 35.498 kb on + strand at 35.498 kb on + strand at 35.499 kb on - strand at 35.614 kb on - strand, within GFF1651 at 35.644 kb on + strand, within GFF1651 at 35.679 kb on + strand, within GFF1651 at 35.727 kb on - strand, within GFF1651 at 35.765 kb on + strand, within GFF1651 at 35.811 kb on + strand, within GFF1651 at 35.822 kb on + strand, within GFF1651 at 36.066 kb on + strand, within GFF1651
Per-strain Table
Position Strand Gene LocusTag Fraction LB_plus_SM_buffer with EIP16_phage 0.0875 MOI remove 34,138 + GFF1648 0.90 +0.1 34,199 - -0.3 34,566 + GFF1649 0.46 +0.3 34,567 - GFF1649 0.46 -0.6 34,578 - GFF1649 0.47 -0.3 34,641 - GFF1649 0.55 -0.3 34,685 + GFF1649 0.60 -0.4 34,686 - GFF1649 0.60 -0.2 35,026 + +0.4 35,027 - -0.5 35,078 + +0.1 35,144 + GFF1650 0.20 -0.5 35,288 - GFF1650 0.57 +0.4 35,424 - -0.6 35,424 - +0.9 35,424 - +0.0 35,426 - -0.3 35,498 + +1.7 35,498 + +0.5 35,499 - +0.0 35,614 - GFF1651 0.15 -0.3 35,644 + GFF1651 0.19 +0.7 35,679 + GFF1651 0.24 -1.2 35,727 - GFF1651 0.31 -1.4 35,765 + GFF1651 0.36 -1.0 35,811 + GFF1651 0.42 -1.1 35,822 + GFF1651 0.43 +0.1 36,066 + GFF1651 0.76 -0.3
Or see this region's nucleotide sequence