Experiment: LB_plus_SM_buffer with EIP16_phage 0.0875 MOI
Only strains with sufficient reads to estimate fitness are shown,
but the strain fitness values are still rather noisy and may be biased towards zero.
Strains near the edge of a gene are not shown as being associated with that
gene (they are in grey).
Strains in the central 10-90% of a gene are color coded by the insertion's strand.
Usually, "+" means that the selectable marker is encoded on the forward strand,
i.e., transcribed rightward.
500 nt GFF1574 and GFF1575 are separated by 89 nucleotides GFF1575 and GFF1576 are separated by 370 nucleotides GFF1576 and GFF1577 are separated by 214 nucleotides GFF1577 and GFF1578 are separated by 34 nucleotides
GFF1574 - corresponds to STY2919 from Accession AL513382: Salmonella typhi CT18, at 35,138 to 35,317
GFF1574
GFF1575 - DNA-binding protein H-NS, at 35,407 to 35,808
GFF1575
GFF1576 - hypothetical protein, at 36,179 to 36,292
GFF1576
GFF1577 - Putative inner membrane protein, at 36,507 to 36,956
GFF1577
GFF1578 - FIG01219827: hypothetical protein, at 36,991 to 37,341
GFF1578
Position (kb) 36
37 Strain fitness (log2 ratio) -1
0
1 at 35.408 kb on + strand at 35.879 kb on + strand at 36.009 kb on - strand at 36.031 kb on + strand at 36.147 kb on - strand at 36.151 kb on + strand at 36.174 kb on + strand at 36.217 kb on + strand, within GFF1576 at 36.422 kb on + strand at 36.448 kb on + strand at 36.503 kb on + strand at 36.795 kb on - strand, within GFF1577 at 36.797 kb on + strand, within GFF1577 at 37.101 kb on - strand, within GFF1578 at 37.167 kb on + strand, within GFF1578 at 37.168 kb on - strand, within GFF1578 at 37.239 kb on - strand, within GFF1578 at 37.239 kb on - strand, within GFF1578 at 37.262 kb on + strand, within GFF1578 at 37.262 kb on + strand, within GFF1578
Per-strain Table
Position Strand Gene LocusTag Fraction LB_plus_SM_buffer with EIP16_phage 0.0875 MOI remove 35,408 + +0.5 35,879 + +1.1 36,009 - -0.4 36,031 + -0.1 36,147 - -0.6 36,151 + +0.5 36,174 + +0.6 36,217 + GFF1576 0.33 -0.1 36,422 + -0.2 36,448 + -0.2 36,503 + +0.1 36,795 - GFF1577 0.64 -0.1 36,797 + GFF1577 0.64 +0.6 37,101 - GFF1578 0.31 +0.4 37,167 + GFF1578 0.50 +0.1 37,168 - GFF1578 0.50 -0.0 37,239 - GFF1578 0.71 +0.5 37,239 - GFF1578 0.71 +0.4 37,262 + GFF1578 0.77 +0.2 37,262 + GFF1578 0.77 -0.1
Or see this region's nucleotide sequence