Strain Fitness in Salmonella enterica subsp. enterica serovar Typhimurium str. MS1868 around GFF1423

Experiment: LB_plus_SM_buffer with EIP16_phage 0.0875 MOI

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Only strains with sufficient reads to estimate fitness are shown, but the strain fitness values are still rather noisy and may be biased towards zero. Strains near the edge of a gene are not shown as being associated with that gene (they are in grey). Strains in the central 10-90% of a gene are color coded by the insertion's strand. Usually, "+" means that the selectable marker is encoded on the forward strand, i.e., transcribed rightward.

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500 ntGFF1422 and GFF1423 are separated by 185 nucleotidesGFF1423 and GFF1424 are separated by 186 nucleotides GFF1422 - Alanine racemase (EC 5.1.1.1), at 68,169 to 69,248 GFF1422 GFF1423 - Biosynthetic Aromatic amino acid aminotransferase alpha (EC 2.6.1.57), at 69,434 to 70,627 GFF1423 GFF1424 - NMN phosphatase (EC 3.1.3.5); Class B acid phosphatase precursor (EC 3.1.3.2), at 70,814 to 71,527 GFF1424 Position (kb) 69 70 71Strain fitness (log2 ratio) -1 0 1 2at 68.457 kb on - strand, within GFF1422at 68.465 kb on - strand, within GFF1422at 68.465 kb on - strand, within GFF1422at 68.589 kb on + strand, within GFF1422at 68.633 kb on + strand, within GFF1422at 68.721 kb on - strand, within GFF1422at 68.721 kb on - strand, within GFF1422at 68.738 kb on + strand, within GFF1422at 69.167 kb on + strandat 69.195 kb on + strandat 69.203 kb on + strandat 69.237 kb on + strandat 69.238 kb on - strandat 69.239 kb on + strandat 69.343 kb on - strandat 69.390 kb on + strandat 69.456 kb on - strandat 69.559 kb on + strand, within GFF1423at 69.559 kb on + strand, within GFF1423at 69.630 kb on - strand, within GFF1423at 69.652 kb on + strand, within GFF1423at 69.653 kb on - strand, within GFF1423at 69.805 kb on - strand, within GFF1423at 70.112 kb on + strand, within GFF1423at 70.112 kb on + strand, within GFF1423at 70.283 kb on + strand, within GFF1423at 70.390 kb on + strand, within GFF1423at 70.390 kb on + strand, within GFF1423at 70.390 kb on + strand, within GFF1423at 70.392 kb on + strand, within GFF1423at 70.393 kb on - strand, within GFF1423at 70.454 kb on + strand, within GFF1423at 70.461 kb on + strand, within GFF1423at 70.485 kb on - strand, within GFF1423at 70.486 kb on + strand, within GFF1423at 70.487 kb on - strand, within GFF1423at 70.576 kb on + strandat 70.591 kb on - strandat 70.626 kb on - strandat 70.672 kb on - strandat 70.672 kb on - strandat 70.672 kb on - strandat 70.672 kb on - strandat 70.887 kb on + strand, within GFF1424at 71.097 kb on - strand, within GFF1424at 71.214 kb on - strand, within GFF1424at 71.214 kb on - strand, within GFF1424at 71.214 kb on - strand, within GFF1424at 71.401 kb on + strand, within GFF1424at 71.402 kb on - strand, within GFF1424at 71.620 kb on - strand

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Per-strain Table

Position Strand Gene LocusTag Fraction LB_plus_SM_buffer with EIP16_phage 0.0875 MOI
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68,457 - GFF1422 0.27 +0.1
68,465 - GFF1422 0.27 +0.1
68,465 - GFF1422 0.27 -0.4
68,589 + GFF1422 0.39 -0.2
68,633 + GFF1422 0.43 -0.8
68,721 - GFF1422 0.51 -0.8
68,721 - GFF1422 0.51 -0.1
68,738 + GFF1422 0.53 +1.9
69,167 + -1.0
69,195 + -0.1
69,203 + -0.4
69,237 + -0.3
69,238 - +1.0
69,239 + +1.1
69,343 - -0.3
69,390 + +1.6
69,456 - -0.1
69,559 + GFF1423 0.10 -0.8
69,559 + GFF1423 0.10 -0.9
69,630 - GFF1423 0.16 -1.1
69,652 + GFF1423 0.18 -0.7
69,653 - GFF1423 0.18 -0.7
69,805 - GFF1423 0.31 -0.4
70,112 + GFF1423 0.57 -0.3
70,112 + GFF1423 0.57 -0.7
70,283 + GFF1423 0.71 -1.6
70,390 + GFF1423 0.80 -0.6
70,390 + GFF1423 0.80 -0.6
70,390 + GFF1423 0.80 -0.2
70,392 + GFF1423 0.80 -1.6
70,393 - GFF1423 0.80 -0.4
70,454 + GFF1423 0.85 -0.7
70,461 + GFF1423 0.86 -0.2
70,485 - GFF1423 0.88 -1.0
70,486 + GFF1423 0.88 -0.5
70,487 - GFF1423 0.88 -0.7
70,576 + -0.4
70,591 - +0.3
70,626 - -0.1
70,672 - -0.2
70,672 - -0.2
70,672 - -0.0
70,672 - -1.0
70,887 + GFF1424 0.10 +0.5
71,097 - GFF1424 0.40 -0.6
71,214 - GFF1424 0.56 -0.2
71,214 - GFF1424 0.56 +0.4
71,214 - GFF1424 0.56 -0.2
71,401 + GFF1424 0.82 +0.2
71,402 - GFF1424 0.82 +0.3
71,620 - +0.0

Or see this region's nucleotide sequence