Experiment: LB_plus_SM_buffer with EIP16_phage 0.0875 MOI
Only strains with sufficient reads to estimate fitness are shown,
but the strain fitness values are still rather noisy and may be biased towards zero.
Strains near the edge of a gene are not shown as being associated with that
gene (they are in grey).
Strains in the central 10-90% of a gene are color coded by the insertion's strand.
Usually, "+" means that the selectable marker is encoded on the forward strand,
i.e., transcribed rightward.
500 nt GFF1312 and GFF1313 are separated by 521 nucleotides GFF1313 and GFF1314 are separated by 35 nucleotides GFF1314 and GFF1315 are separated by 181 nucleotides
GFF1312 - 'Adenosine (5')-pentaphospho-(5'')-adenosine pyrophosphohydrolase (EC 3.6.1.-)' transl_table=11, at 20,976 to 21,506
GFF1312
GFF1313 - hypothetical protein, at 22,028 to 22,153
GFF1313
GFF1314 - DNA mismatch repair endonuclease MutH, at 22,189 to 22,884
GFF1314
GFF1315 - FIG003462: membrane protein, at 23,066 to 23,779
GFF1315
Position (kb)
22
23 Strain fitness (log2 ratio)
-1
0
1
2 at 21.195 kb on + strand, within GFF1312 at 21.328 kb on - strand, within GFF1312 at 21.465 kb on + strand at 21.474 kb on + strand at 21.474 kb on + strand at 21.475 kb on - strand at 21.512 kb on + strand at 21.523 kb on - strand at 21.529 kb on + strand at 21.530 kb on - strand at 21.530 kb on - strand at 21.530 kb on - strand at 21.530 kb on - strand at 21.532 kb on - strand at 21.532 kb on - strand at 21.532 kb on - strand at 21.533 kb on + strand at 21.534 kb on - strand at 21.534 kb on - strand at 21.550 kb on + strand at 21.582 kb on - strand at 21.802 kb on + strand at 21.803 kb on - strand at 21.946 kb on + strand at 21.947 kb on - strand at 22.003 kb on + strand at 22.003 kb on + strand at 22.008 kb on + strand at 22.009 kb on - strand at 22.020 kb on + strand at 22.151 kb on + strand at 22.187 kb on + strand at 22.241 kb on + strand at 22.269 kb on + strand, within GFF1314 at 22.269 kb on + strand, within GFF1314 at 22.269 kb on + strand, within GFF1314 at 22.269 kb on + strand, within GFF1314 at 22.269 kb on + strand, within GFF1314 at 22.270 kb on - strand, within GFF1314 at 22.270 kb on - strand, within GFF1314 at 22.416 kb on - strand, within GFF1314 at 22.580 kb on + strand, within GFF1314 at 22.649 kb on + strand, within GFF1314 at 22.682 kb on + strand, within GFF1314 at 22.683 kb on - strand, within GFF1314 at 22.798 kb on - strand, within GFF1314 at 22.919 kb on + strand at 22.947 kb on + strand at 22.947 kb on + strand at 22.948 kb on - strand at 22.953 kb on + strand at 23.029 kb on - strand at 23.030 kb on + strand at 23.035 kb on - strand at 23.070 kb on + strand at 23.070 kb on + strand at 23.137 kb on - strand at 23.137 kb on - strand at 23.137 kb on - strand at 23.156 kb on - strand, within GFF1315 at 23.240 kb on - strand, within GFF1315 at 23.476 kb on + strand, within GFF1315 at 23.477 kb on - strand, within GFF1315 at 23.530 kb on + strand, within GFF1315 at 23.534 kb on - strand, within GFF1315 at 23.704 kb on + strand, within GFF1315 at 23.704 kb on + strand, within GFF1315 at 23.708 kb on + strand at 23.759 kb on - strand at 23.810 kb on + strand at 23.820 kb on - strand at 23.884 kb on + strand
Per-strain Table
Position Strand Gene LocusTag Fraction LB_plus_SM_buffer with EIP16_phage 0.0875 MOI remove 21,195 + GFF1312 0.41 +1.2 21,328 - GFF1312 0.66 +0.2 21,465 + -0.4 21,474 + -0.4 21,474 + -0.2 21,475 - +0.5 21,512 + -0.5 21,523 - +0.9 21,529 + +0.3 21,530 - +0.3 21,530 - +0.3 21,530 - -0.2 21,530 - +0.4 21,532 - +0.9 21,532 - +0.9 21,532 - +0.4 21,533 + -1.2 21,534 - +0.5 21,534 - +0.1 21,550 + +0.4 21,582 - +1.2 21,802 + -1.0 21,803 - +0.4 21,946 + -0.6 21,947 - +1.6 22,003 + -0.5 22,003 + +1.7 22,008 + -0.2 22,009 - -0.6 22,020 + +0.1 22,151 + -0.2 22,187 + -0.5 22,241 + -0.8 22,269 + GFF1314 0.11 -0.4 22,269 + GFF1314 0.11 -0.1 22,269 + GFF1314 0.11 -0.2 22,269 + GFF1314 0.11 -0.1 22,269 + GFF1314 0.11 +0.3 22,270 - GFF1314 0.12 +0.3 22,270 - GFF1314 0.12 -1.1 22,416 - GFF1314 0.33 -0.1 22,580 + GFF1314 0.56 +0.3 22,649 + GFF1314 0.66 -0.6 22,682 + GFF1314 0.71 -0.1 22,683 - GFF1314 0.71 -0.3 22,798 - GFF1314 0.88 -0.2 22,919 + -0.2 22,947 + +0.0 22,947 + -0.8 22,948 - -1.0 22,953 + +0.3 23,029 - -0.6 23,030 + +0.9 23,035 - -1.1 23,070 + +2.7 23,070 + -0.8 23,137 - -0.0 23,137 - +0.0 23,137 - -0.2 23,156 - GFF1315 0.13 +0.6 23,240 - GFF1315 0.24 +0.3 23,476 + GFF1315 0.57 -0.2 23,477 - GFF1315 0.58 -0.8 23,530 + GFF1315 0.65 -0.3 23,534 - GFF1315 0.66 -0.4 23,704 + GFF1315 0.89 +0.3 23,704 + GFF1315 0.89 +0.2 23,708 + -0.1 23,759 - -0.2 23,810 + -0.0 23,820 - +0.1 23,884 + +0.2
Or see this region's nucleotide sequence