Strain Fitness in Salmonella enterica subsp. enterica serovar Typhimurium str. MS1868 around GFF1314

Experiment: LB_plus_SM_buffer with EIP16_phage 0.0875 MOI

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Only strains with sufficient reads to estimate fitness are shown, but the strain fitness values are still rather noisy and may be biased towards zero. Strains near the edge of a gene are not shown as being associated with that gene (they are in grey). Strains in the central 10-90% of a gene are color coded by the insertion's strand. Usually, "+" means that the selectable marker is encoded on the forward strand, i.e., transcribed rightward.

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500 ntGFF1312 and GFF1313 are separated by 521 nucleotidesGFF1313 and GFF1314 are separated by 35 nucleotidesGFF1314 and GFF1315 are separated by 181 nucleotides GFF1312 - 'Adenosine (5')-pentaphospho-(5'')-adenosine pyrophosphohydrolase (EC 3.6.1.-)' transl_table=11, at 20,976 to 21,506 GFF1312 GFF1313 - hypothetical protein, at 22,028 to 22,153 GFF1313 GFF1314 - DNA mismatch repair endonuclease MutH, at 22,189 to 22,884 GFF1314 GFF1315 - FIG003462: membrane protein, at 23,066 to 23,779 GFF1315 Position (kb) 22 23Strain fitness (log2 ratio) -1 0 1 2at 21.195 kb on + strand, within GFF1312at 21.328 kb on - strand, within GFF1312at 21.465 kb on + strandat 21.474 kb on + strandat 21.474 kb on + strandat 21.475 kb on - strandat 21.512 kb on + strandat 21.523 kb on - strandat 21.529 kb on + strandat 21.530 kb on - strandat 21.530 kb on - strandat 21.530 kb on - strandat 21.530 kb on - strandat 21.532 kb on - strandat 21.532 kb on - strandat 21.532 kb on - strandat 21.533 kb on + strandat 21.534 kb on - strandat 21.534 kb on - strandat 21.550 kb on + strandat 21.582 kb on - strandat 21.802 kb on + strandat 21.803 kb on - strandat 21.946 kb on + strandat 21.947 kb on - strandat 22.003 kb on + strandat 22.003 kb on + strandat 22.008 kb on + strandat 22.009 kb on - strandat 22.020 kb on + strandat 22.151 kb on + strandat 22.187 kb on + strandat 22.241 kb on + strandat 22.269 kb on + strand, within GFF1314at 22.269 kb on + strand, within GFF1314at 22.269 kb on + strand, within GFF1314at 22.269 kb on + strand, within GFF1314at 22.269 kb on + strand, within GFF1314at 22.270 kb on - strand, within GFF1314at 22.270 kb on - strand, within GFF1314at 22.416 kb on - strand, within GFF1314at 22.580 kb on + strand, within GFF1314at 22.649 kb on + strand, within GFF1314at 22.682 kb on + strand, within GFF1314at 22.683 kb on - strand, within GFF1314at 22.798 kb on - strand, within GFF1314at 22.919 kb on + strandat 22.947 kb on + strandat 22.947 kb on + strandat 22.948 kb on - strandat 22.953 kb on + strandat 23.029 kb on - strandat 23.030 kb on + strandat 23.035 kb on - strandat 23.070 kb on + strandat 23.070 kb on + strandat 23.137 kb on - strandat 23.137 kb on - strandat 23.137 kb on - strandat 23.156 kb on - strand, within GFF1315at 23.240 kb on - strand, within GFF1315at 23.476 kb on + strand, within GFF1315at 23.477 kb on - strand, within GFF1315at 23.530 kb on + strand, within GFF1315at 23.534 kb on - strand, within GFF1315at 23.704 kb on + strand, within GFF1315at 23.704 kb on + strand, within GFF1315at 23.708 kb on + strandat 23.759 kb on - strandat 23.810 kb on + strandat 23.820 kb on - strandat 23.884 kb on + strand

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Per-strain Table

Position Strand Gene LocusTag Fraction LB_plus_SM_buffer with EIP16_phage 0.0875 MOI
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21,195 + GFF1312 0.41 +1.2
21,328 - GFF1312 0.66 +0.2
21,465 + -0.4
21,474 + -0.4
21,474 + -0.2
21,475 - +0.5
21,512 + -0.5
21,523 - +0.9
21,529 + +0.3
21,530 - +0.3
21,530 - +0.3
21,530 - -0.2
21,530 - +0.4
21,532 - +0.9
21,532 - +0.9
21,532 - +0.4
21,533 + -1.2
21,534 - +0.5
21,534 - +0.1
21,550 + +0.4
21,582 - +1.2
21,802 + -1.0
21,803 - +0.4
21,946 + -0.6
21,947 - +1.6
22,003 + -0.5
22,003 + +1.7
22,008 + -0.2
22,009 - -0.6
22,020 + +0.1
22,151 + -0.2
22,187 + -0.5
22,241 + -0.8
22,269 + GFF1314 0.11 -0.4
22,269 + GFF1314 0.11 -0.1
22,269 + GFF1314 0.11 -0.2
22,269 + GFF1314 0.11 -0.1
22,269 + GFF1314 0.11 +0.3
22,270 - GFF1314 0.12 +0.3
22,270 - GFF1314 0.12 -1.1
22,416 - GFF1314 0.33 -0.1
22,580 + GFF1314 0.56 +0.3
22,649 + GFF1314 0.66 -0.6
22,682 + GFF1314 0.71 -0.1
22,683 - GFF1314 0.71 -0.3
22,798 - GFF1314 0.88 -0.2
22,919 + -0.2
22,947 + +0.0
22,947 + -0.8
22,948 - -1.0
22,953 + +0.3
23,029 - -0.6
23,030 + +0.9
23,035 - -1.1
23,070 + +2.7
23,070 + -0.8
23,137 - -0.0
23,137 - +0.0
23,137 - -0.2
23,156 - GFF1315 0.13 +0.6
23,240 - GFF1315 0.24 +0.3
23,476 + GFF1315 0.57 -0.2
23,477 - GFF1315 0.58 -0.8
23,530 + GFF1315 0.65 -0.3
23,534 - GFF1315 0.66 -0.4
23,704 + GFF1315 0.89 +0.3
23,704 + GFF1315 0.89 +0.2
23,708 + -0.1
23,759 - -0.2
23,810 + -0.0
23,820 - +0.1
23,884 + +0.2

Or see this region's nucleotide sequence