Experiment: LB_plus_SM_buffer with EIP16_phage 0.0875 MOI
Only strains with sufficient reads to estimate fitness are shown,
but the strain fitness values are still rather noisy and may be biased towards zero.
Strains near the edge of a gene are not shown as being associated with that
gene (they are in grey).
Strains in the central 10-90% of a gene are color coded by the insertion's strand.
Usually, "+" means that the selectable marker is encoded on the forward strand,
i.e., transcribed rightward.
500 nt GFF1177 and GFF1178 overlap by 32 nucleotides GFF1178 and GFF1179 are separated by 113 nucleotides GFF1179 and GFF1180 overlap by 14 nucleotides GFF1180 and GFF1181 overlap by 11 nucleotides
GFF1177 - IncF plasmid conjugative transfer pilus assembly protein TraF, at 22,968 to 23,720
GFF1177
GFF1178 - hypothetical protein, at 23,689 to 23,820
GFF1178
GFF1179 - IncF plasmid conjugative transfer protein TraQ, at 23,934 to 24,227
GFF1179
GFF1180 - IncF plasmid conjugative transfer protein TrbB, at 24,214 to 24,762
GFF1180
GFF1181 - IncF plasmid conjugative transfer pilus assembly protein TraH, at 24,752 to 26,134
GFF1181
Position (kb) 24
25 Strain fitness (log2 ratio) -1
0
1 at 23.217 kb on + strand, within GFF1177 at 23.307 kb on + strand, within GFF1177 at 23.307 kb on + strand, within GFF1177 at 23.308 kb on - strand, within GFF1177 at 23.308 kb on - strand, within GFF1177 at 23.319 kb on + strand, within GFF1177 at 23.329 kb on - strand, within GFF1177 at 23.341 kb on + strand, within GFF1177 at 23.342 kb on - strand, within GFF1177 at 23.406 kb on + strand, within GFF1177 at 23.407 kb on - strand, within GFF1177 at 23.412 kb on - strand, within GFF1177 at 23.425 kb on - strand, within GFF1177 at 23.468 kb on + strand, within GFF1177 at 23.487 kb on + strand, within GFF1177 at 23.487 kb on + strand, within GFF1177 at 23.487 kb on + strand, within GFF1177 at 23.612 kb on - strand, within GFF1177 at 23.655 kb on - strand at 23.739 kb on + strand, within GFF1178 at 23.739 kb on + strand, within GFF1178 at 23.905 kb on - strand at 23.907 kb on - strand at 24.226 kb on - strand at 24.303 kb on + strand, within GFF1180 at 24.489 kb on - strand, within GFF1180 at 24.512 kb on - strand, within GFF1180 at 24.534 kb on + strand, within GFF1180 at 24.671 kb on + strand, within GFF1180 at 24.672 kb on - strand, within GFF1180 at 24.739 kb on + strand at 24.740 kb on - strand at 24.740 kb on - strand at 24.740 kb on - strand at 24.740 kb on - strand at 24.740 kb on - strand at 24.760 kb on + strand at 24.772 kb on + strand at 24.772 kb on + strand at 24.786 kb on + strand at 24.787 kb on - strand at 24.789 kb on + strand at 24.862 kb on + strand at 24.924 kb on - strand, within GFF1181 at 24.942 kb on + strand, within GFF1181 at 24.943 kb on - strand, within GFF1181 at 24.943 kb on - strand, within GFF1181 at 24.943 kb on - strand, within GFF1181 at 24.943 kb on - strand, within GFF1181 at 24.943 kb on - strand, within GFF1181 at 24.945 kb on - strand, within GFF1181 at 25.029 kb on - strand, within GFF1181 at 25.029 kb on - strand, within GFF1181 at 25.087 kb on + strand, within GFF1181 at 25.088 kb on - strand, within GFF1181 at 25.093 kb on + strand, within GFF1181 at 25.189 kb on + strand, within GFF1181 at 25.195 kb on + strand, within GFF1181 at 25.195 kb on + strand, within GFF1181 at 25.339 kb on + strand, within GFF1181 at 25.339 kb on + strand, within GFF1181 at 25.339 kb on + strand, within GFF1181 at 25.339 kb on + strand, within GFF1181 at 25.340 kb on - strand, within GFF1181 at 25.565 kb on - strand, within GFF1181 at 25.600 kb on - strand, within GFF1181 at 25.600 kb on - strand, within GFF1181 at 25.601 kb on + strand, within GFF1181 at 25.601 kb on + strand, within GFF1181 at 25.602 kb on - strand, within GFF1181 at 25.602 kb on - strand, within GFF1181 at 25.621 kb on + strand, within GFF1181 at 25.747 kb on + strand, within GFF1181 at 25.762 kb on + strand, within GFF1181
Per-strain Table
Position Strand Gene LocusTag Fraction LB_plus_SM_buffer with EIP16_phage 0.0875 MOI remove 23,217 + GFF1177 0.33 +1.2 23,307 + GFF1177 0.45 -0.1 23,307 + GFF1177 0.45 -0.1 23,308 - GFF1177 0.45 -0.1 23,308 - GFF1177 0.45 +0.1 23,319 + GFF1177 0.47 -0.4 23,329 - GFF1177 0.48 -0.4 23,341 + GFF1177 0.50 -0.4 23,342 - GFF1177 0.50 +0.1 23,406 + GFF1177 0.58 -1.4 23,407 - GFF1177 0.58 -0.2 23,412 - GFF1177 0.59 +0.2 23,425 - GFF1177 0.61 -0.3 23,468 + GFF1177 0.66 -0.0 23,487 + GFF1177 0.69 -0.8 23,487 + GFF1177 0.69 -0.7 23,487 + GFF1177 0.69 -0.1 23,612 - GFF1177 0.86 +0.4 23,655 - -1.4 23,739 + GFF1178 0.38 +0.4 23,739 + GFF1178 0.38 +1.1 23,905 - -0.1 23,907 - +0.1 24,226 - +0.1 24,303 + GFF1180 0.16 -0.0 24,489 - GFF1180 0.50 -0.3 24,512 - GFF1180 0.54 +0.3 24,534 + GFF1180 0.58 +1.1 24,671 + GFF1180 0.83 +0.3 24,672 - GFF1180 0.83 +0.3 24,739 + +0.1 24,740 - +0.8 24,740 - +0.2 24,740 - +0.1 24,740 - +0.3 24,740 - -0.7 24,760 + +0.0 24,772 + +1.5 24,772 + +0.1 24,786 + +0.6 24,787 - +1.4 24,789 + +0.6 24,862 + +1.0 24,924 - GFF1181 0.12 +0.2 24,942 + GFF1181 0.14 +0.2 24,943 - GFF1181 0.14 -0.0 24,943 - GFF1181 0.14 +1.0 24,943 - GFF1181 0.14 -0.4 24,943 - GFF1181 0.14 -0.5 24,943 - GFF1181 0.14 -0.4 24,945 - GFF1181 0.14 -0.1 25,029 - GFF1181 0.20 +1.3 25,029 - GFF1181 0.20 -0.3 25,087 + GFF1181 0.24 +0.5 25,088 - GFF1181 0.24 +0.4 25,093 + GFF1181 0.25 -0.3 25,189 + GFF1181 0.32 -0.3 25,195 + GFF1181 0.32 +0.4 25,195 + GFF1181 0.32 +0.3 25,339 + GFF1181 0.42 -0.0 25,339 + GFF1181 0.42 -0.2 25,339 + GFF1181 0.42 +1.0 25,339 + GFF1181 0.42 -0.3 25,340 - GFF1181 0.43 -0.1 25,565 - GFF1181 0.59 -0.1 25,600 - GFF1181 0.61 +0.0 25,600 - GFF1181 0.61 -0.7 25,601 + GFF1181 0.61 +0.3 25,601 + GFF1181 0.61 -0.3 25,602 - GFF1181 0.61 +0.0 25,602 - GFF1181 0.61 -0.5 25,621 + GFF1181 0.63 -0.5 25,747 + GFF1181 0.72 -0.4 25,762 + GFF1181 0.73 +0.2
Or see this region's nucleotide sequence