Experiment: R2A with Nalidixic 0.02 mM
Only strains with sufficient reads to estimate fitness are shown,
but the strain fitness values are still rather noisy and may be biased towards zero.
Strains near the edge of a gene are not shown as being associated with that
gene (they are in grey).
Strains in the central 10-90% of a gene are color coded by the insertion's strand.
Usually, "+" means that the selectable marker is encoded on the forward strand,
i.e., transcribed rightward.
500 nt Atu2590 and Atu2591 are separated by 163 nucleotides Atu2591 and Atu2592 overlap by 4 nucleotides
Atu2590: Atu2590 - hypothetical protein, at 2,567,299 to 2,568,507
Atu2590
Atu2591: Atu2591 - glucosyltransferase, at 2,568,671 to 2,569,798
Atu2591
Atu2592: Atu2592 - hypothetical protein, at 2,569,795 to 2,570,946
Atu2592
Position (kb)
2568
2569
2570 Strain fitness (log2 ratio)
-4
-3
-2
-1
0
1
2
3
4 at 2567.705 kb on + strand, within Atu2590 at 2567.713 kb on - strand, within Atu2590 at 2567.713 kb on - strand, within Atu2590 at 2567.740 kb on - strand, within Atu2590 at 2567.866 kb on + strand, within Atu2590 at 2567.916 kb on + strand, within Atu2590 at 2568.019 kb on - strand, within Atu2590 at 2568.180 kb on - strand, within Atu2590 at 2568.259 kb on + strand, within Atu2590 at 2568.304 kb on - strand, within Atu2590 at 2568.356 kb on + strand, within Atu2590 at 2568.384 kb on - strand, within Atu2590 at 2568.384 kb on - strand, within Atu2590 at 2568.415 kb on - strand at 2568.474 kb on - strand at 2568.542 kb on - strand at 2568.588 kb on - strand at 2568.702 kb on - strand at 2568.727 kb on + strand at 2568.894 kb on - strand, within Atu2591 at 2568.976 kb on - strand, within Atu2591 at 2568.979 kb on + strand, within Atu2591 at 2568.987 kb on - strand, within Atu2591 at 2568.989 kb on + strand, within Atu2591 at 2569.014 kb on + strand, within Atu2591 at 2569.041 kb on + strand, within Atu2591 at 2569.087 kb on - strand, within Atu2591 at 2569.161 kb on + strand, within Atu2591 at 2569.194 kb on - strand, within Atu2591 at 2569.209 kb on + strand, within Atu2591 at 2569.217 kb on - strand, within Atu2591 at 2569.305 kb on + strand, within Atu2591 at 2569.360 kb on - strand, within Atu2591 at 2569.467 kb on + strand, within Atu2591 at 2569.528 kb on + strand, within Atu2591 at 2569.531 kb on - strand, within Atu2591 at 2569.555 kb on - strand, within Atu2591 at 2569.555 kb on - strand, within Atu2591 at 2569.768 kb on + strand at 2569.768 kb on + strand at 2569.768 kb on + strand at 2569.768 kb on + strand at 2569.776 kb on - strand at 2569.845 kb on + strand at 2569.999 kb on - strand, within Atu2592 at 2570.027 kb on + strand, within Atu2592 at 2570.069 kb on - strand, within Atu2592 at 2570.203 kb on - strand, within Atu2592 at 2570.238 kb on + strand, within Atu2592 at 2570.266 kb on - strand, within Atu2592 at 2570.269 kb on + strand, within Atu2592 at 2570.270 kb on + strand, within Atu2592 at 2570.273 kb on + strand, within Atu2592 at 2570.341 kb on - strand, within Atu2592 at 2570.388 kb on - strand, within Atu2592 at 2570.481 kb on - strand, within Atu2592 at 2570.486 kb on - strand, within Atu2592 at 2570.489 kb on + strand, within Atu2592 at 2570.693 kb on - strand, within Atu2592
Per-strain Table
Position Strand Gene LocusTag Fraction R2A with Nalidixic 0.02 mM remove 2,567,705 + Atu2590 0.34 -1.6 2,567,713 - Atu2590 0.34 -0.5 2,567,713 - Atu2590 0.34 -2.9 2,567,740 - Atu2590 0.36 -1.2 2,567,866 + Atu2590 0.47 -3.2 2,567,916 + Atu2590 0.51 -3.8 2,568,019 - Atu2590 0.60 -3.4 2,568,180 - Atu2590 0.73 -2.7 2,568,259 + Atu2590 0.79 -3.0 2,568,304 - Atu2590 0.83 +4.7 2,568,356 + Atu2590 0.87 +0.1 2,568,384 - Atu2590 0.90 -3.4 2,568,384 - Atu2590 0.90 -2.6 2,568,415 - +0.1 2,568,474 - -1.2 2,568,542 - -0.1 2,568,588 - -3.5 2,568,702 - -0.7 2,568,727 + -1.5 2,568,894 - Atu2591 0.20 -2.1 2,568,976 - Atu2591 0.27 -2.5 2,568,979 + Atu2591 0.27 -2.8 2,568,987 - Atu2591 0.28 -1.8 2,568,989 + Atu2591 0.28 +0.5 2,569,014 + Atu2591 0.30 -3.2 2,569,041 + Atu2591 0.33 -3.0 2,569,087 - Atu2591 0.37 -2.7 2,569,161 + Atu2591 0.43 -2.0 2,569,194 - Atu2591 0.46 -0.9 2,569,209 + Atu2591 0.48 -3.4 2,569,217 - Atu2591 0.48 -1.5 2,569,305 + Atu2591 0.56 -1.1 2,569,360 - Atu2591 0.61 +3.1 2,569,467 + Atu2591 0.71 +1.1 2,569,528 + Atu2591 0.76 +2.1 2,569,531 - Atu2591 0.76 +2.1 2,569,555 - Atu2591 0.78 -1.5 2,569,555 - Atu2591 0.78 +1.1 2,569,768 + +0.3 2,569,768 + -0.0 2,569,768 + -1.0 2,569,768 + -0.1 2,569,776 - -2.4 2,569,845 + -2.2 2,569,999 - Atu2592 0.18 -0.9 2,570,027 + Atu2592 0.20 +0.1 2,570,069 - Atu2592 0.24 +0.1 2,570,203 - Atu2592 0.35 -4.0 2,570,238 + Atu2592 0.38 -2.9 2,570,266 - Atu2592 0.41 -2.1 2,570,269 + Atu2592 0.41 +2.1 2,570,270 + Atu2592 0.41 -3.0 2,570,273 + Atu2592 0.41 -0.5 2,570,341 - Atu2592 0.47 +0.5 2,570,388 - Atu2592 0.51 -0.5 2,570,481 - Atu2592 0.60 -2.0 2,570,486 - Atu2592 0.60 +0.1 2,570,489 + Atu2592 0.60 +0.1 2,570,693 - Atu2592 0.78 -1.3
Or see this region's nucleotide sequence