Experiment: R2A with Nalidixic 0.02 mM
Only strains with sufficient reads to estimate fitness are shown,
but the strain fitness values are still rather noisy and may be biased towards zero.
Strains near the edge of a gene are not shown as being associated with that
gene (they are in grey).
Strains in the central 10-90% of a gene are color coded by the insertion's strand.
Usually, "+" means that the selectable marker is encoded on the forward strand,
i.e., transcribed rightward.
500 nt Atu2573 and Atu2574 are separated by 342 nucleotides Atu2574 and Atu2575 overlap by 4 nucleotides Atu2575 and Atu2576 overlap by 33 nucleotides
Atu2573: Atu2573 - hypothetical protein, at 2,543,770 to 2,544,465
Atu2573
Atu2574: Atu2574 - hypothetical protein, at 2,544,808 to 2,545,455
Atu2574
Atu2575: Atu2575 - mannosidase, at 2,545,452 to 2,547,908
Atu2575
Atu2576: Atu2576 - hypothetical protein, at 2,547,876 to 2,549,735
Atu2576
Position (kb)
2545
2546
2547
2548 Strain fitness (log2 ratio)
-1
0
1 at 2544.618 kb on + strand at 2544.800 kb on + strand at 2544.811 kb on + strand at 2544.826 kb on - strand at 2544.833 kb on + strand at 2544.854 kb on - strand at 2545.023 kb on + strand, within Atu2574 at 2545.247 kb on - strand, within Atu2574 at 2545.259 kb on - strand, within Atu2574 at 2545.305 kb on + strand, within Atu2574 at 2545.305 kb on + strand, within Atu2574 at 2545.305 kb on + strand, within Atu2574 at 2545.374 kb on + strand, within Atu2574 at 2545.411 kb on - strand at 2545.432 kb on + strand at 2545.499 kb on - strand at 2545.589 kb on - strand at 2545.693 kb on + strand at 2545.710 kb on - strand, within Atu2575 at 2545.781 kb on - strand, within Atu2575 at 2545.819 kb on + strand, within Atu2575 at 2545.928 kb on + strand, within Atu2575 at 2545.933 kb on + strand, within Atu2575 at 2545.933 kb on + strand, within Atu2575 at 2545.939 kb on - strand, within Atu2575 at 2545.941 kb on + strand, within Atu2575 at 2545.986 kb on + strand, within Atu2575 at 2546.027 kb on + strand, within Atu2575 at 2546.035 kb on + strand, within Atu2575 at 2546.035 kb on - strand, within Atu2575 at 2546.042 kb on + strand, within Atu2575 at 2546.072 kb on + strand, within Atu2575 at 2546.106 kb on - strand, within Atu2575 at 2546.237 kb on + strand, within Atu2575 at 2546.245 kb on - strand, within Atu2575 at 2546.250 kb on - strand, within Atu2575 at 2546.300 kb on + strand, within Atu2575 at 2546.308 kb on - strand, within Atu2575 at 2546.308 kb on - strand, within Atu2575 at 2546.353 kb on - strand, within Atu2575 at 2546.353 kb on - strand, within Atu2575 at 2546.386 kb on - strand, within Atu2575 at 2546.386 kb on - strand, within Atu2575 at 2546.726 kb on + strand, within Atu2575 at 2546.836 kb on + strand, within Atu2575 at 2546.969 kb on + strand, within Atu2575 at 2546.977 kb on - strand, within Atu2575 at 2547.083 kb on + strand, within Atu2575 at 2547.143 kb on + strand, within Atu2575 at 2547.295 kb on - strand, within Atu2575 at 2547.330 kb on + strand, within Atu2575 at 2547.343 kb on - strand, within Atu2575 at 2547.387 kb on - strand, within Atu2575 at 2547.446 kb on - strand, within Atu2575 at 2547.477 kb on - strand, within Atu2575 at 2547.500 kb on - strand, within Atu2575 at 2547.539 kb on + strand, within Atu2575 at 2547.547 kb on - strand, within Atu2575 at 2547.547 kb on - strand, within Atu2575 at 2547.736 kb on - strand at 2547.747 kb on + strand at 2547.755 kb on - strand at 2547.772 kb on - strand at 2547.772 kb on - strand at 2547.807 kb on - strand at 2547.968 kb on + strand at 2548.083 kb on - strand, within Atu2576 at 2548.140 kb on + strand, within Atu2576 at 2548.188 kb on - strand, within Atu2576 at 2548.312 kb on + strand, within Atu2576 at 2548.504 kb on + strand, within Atu2576 at 2548.596 kb on + strand, within Atu2576 at 2548.662 kb on + strand, within Atu2576 at 2548.717 kb on + strand, within Atu2576 at 2548.752 kb on + strand, within Atu2576 at 2548.775 kb on + strand, within Atu2576 at 2548.843 kb on + strand, within Atu2576 at 2548.843 kb on + strand, within Atu2576 at 2548.851 kb on - strand, within Atu2576 at 2548.861 kb on - strand, within Atu2576
Per-strain Table
Position Strand Gene LocusTag Fraction R2A with Nalidixic 0.02 mM remove 2,544,618 + +0.3 2,544,800 + -0.6 2,544,811 + +0.2 2,544,826 - +0.3 2,544,833 + -0.7 2,544,854 - +0.9 2,545,023 + Atu2574 0.33 -0.2 2,545,247 - Atu2574 0.68 +0.3 2,545,259 - Atu2574 0.70 -0.8 2,545,305 + Atu2574 0.77 -0.2 2,545,305 + Atu2574 0.77 +0.0 2,545,305 + Atu2574 0.77 -0.1 2,545,374 + Atu2574 0.87 +1.1 2,545,411 - +0.5 2,545,432 + +0.5 2,545,499 - +1.4 2,545,589 - -0.0 2,545,693 + -0.3 2,545,710 - Atu2575 0.11 +0.4 2,545,781 - Atu2575 0.13 +0.0 2,545,819 + Atu2575 0.15 +0.3 2,545,928 + Atu2575 0.19 -0.1 2,545,933 + Atu2575 0.20 -1.7 2,545,933 + Atu2575 0.20 -0.9 2,545,939 - Atu2575 0.20 -0.2 2,545,941 + Atu2575 0.20 -0.3 2,545,986 + Atu2575 0.22 -0.1 2,546,027 + Atu2575 0.23 +0.2 2,546,035 + Atu2575 0.24 -0.0 2,546,035 - Atu2575 0.24 +0.6 2,546,042 + Atu2575 0.24 -0.3 2,546,072 + Atu2575 0.25 -0.6 2,546,106 - Atu2575 0.27 -0.1 2,546,237 + Atu2575 0.32 +0.2 2,546,245 - Atu2575 0.32 -0.1 2,546,250 - Atu2575 0.32 +1.0 2,546,300 + Atu2575 0.35 -0.1 2,546,308 - Atu2575 0.35 +0.3 2,546,308 - Atu2575 0.35 +0.8 2,546,353 - Atu2575 0.37 +0.2 2,546,353 - Atu2575 0.37 -0.6 2,546,386 - Atu2575 0.38 -1.6 2,546,386 - Atu2575 0.38 +0.0 2,546,726 + Atu2575 0.52 +0.6 2,546,836 + Atu2575 0.56 +0.1 2,546,969 + Atu2575 0.62 -0.6 2,546,977 - Atu2575 0.62 -0.3 2,547,083 + Atu2575 0.66 +0.6 2,547,143 + Atu2575 0.69 +0.1 2,547,295 - Atu2575 0.75 -0.1 2,547,330 + Atu2575 0.76 +0.0 2,547,343 - Atu2575 0.77 +0.3 2,547,387 - Atu2575 0.79 -0.7 2,547,446 - Atu2575 0.81 -0.4 2,547,477 - Atu2575 0.82 +0.3 2,547,500 - Atu2575 0.83 +0.1 2,547,539 + Atu2575 0.85 +0.7 2,547,547 - Atu2575 0.85 -0.6 2,547,547 - Atu2575 0.85 +1.0 2,547,736 - +0.1 2,547,747 + -0.1 2,547,755 - -0.3 2,547,772 - -0.5 2,547,772 - +0.7 2,547,807 - +0.9 2,547,968 + +0.4 2,548,083 - Atu2576 0.11 -0.1 2,548,140 + Atu2576 0.14 -0.7 2,548,188 - Atu2576 0.17 -0.0 2,548,312 + Atu2576 0.23 -0.6 2,548,504 + Atu2576 0.34 -0.2 2,548,596 + Atu2576 0.39 -0.1 2,548,662 + Atu2576 0.42 -0.5 2,548,717 + Atu2576 0.45 -0.3 2,548,752 + Atu2576 0.47 -0.2 2,548,775 + Atu2576 0.48 +1.0 2,548,843 + Atu2576 0.52 -0.0 2,548,843 + Atu2576 0.52 -0.2 2,548,851 - Atu2576 0.52 +0.1 2,548,861 - Atu2576 0.53 +0.2
Or see this region's nucleotide sequence