Experiment: LB_plus_SM_buffer with EIP16_phage 0.875 MOI
Only strains with sufficient reads to estimate fitness are shown,
but the strain fitness values are still rather noisy and may be biased towards zero.
Strains near the edge of a gene are not shown as being associated with that
gene (they are in grey).
Strains in the central 10-90% of a gene are color coded by the insertion's strand.
Usually, "+" means that the selectable marker is encoded on the forward strand,
i.e., transcribed rightward.
500 nt GFF828 and GFF829 are separated by 78 nucleotides GFF829 and GFF830 are separated by 102 nucleotides GFF830 and GFF831 are separated by 143 nucleotides GFF831 and GFF832 are separated by 37 nucleotides
GFF828 - HflC protein, at 11,062 to 12,066
GFF828
GFF829 - Putative inner membrane protein YjeT (clustered with HflC), at 12,145 to 12,342
GFF829
GFF830 - Adenylosuccinate synthetase (EC 6.3.4.4), at 12,445 to 13,743
GFF830
GFF831 - Nitrite-sensitive transcriptional repressor NsrR, at 13,887 to 14,375
GFF831
GFF832 - '3'-to-5' exoribonuclease RNase R' transl_table=11, at 14,413 to 16,851
GFF832
Position (kb) 12
13
14 Strain fitness (log2 ratio) -1
0
1
2 at 11.558 kb on + strand, within GFF828 at 11.559 kb on - strand, within GFF828 at 11.652 kb on + strand, within GFF828 at 11.731 kb on + strand, within GFF828 at 11.739 kb on + strand, within GFF828 at 11.869 kb on - strand, within GFF828 at 12.131 kb on + strand at 12.133 kb on + strand at 12.134 kb on - strand at 12.317 kb on - strand, within GFF829 at 12.390 kb on - strand at 13.791 kb on + strand at 13.871 kb on - strand at 13.871 kb on - strand at 13.909 kb on - strand at 13.911 kb on - strand at 13.911 kb on - strand at 13.981 kb on + strand, within GFF831 at 13.982 kb on - strand, within GFF831 at 13.996 kb on - strand, within GFF831 at 13.996 kb on - strand, within GFF831 at 14.052 kb on + strand, within GFF831 at 14.223 kb on - strand, within GFF831 at 14.313 kb on + strand, within GFF831 at 14.548 kb on - strand at 14.638 kb on + strand at 14.708 kb on + strand, within GFF832 at 14.738 kb on - strand, within GFF832
Per-strain Table
Position Strand Gene LocusTag Fraction LB_plus_SM_buffer with EIP16_phage 0.875 MOI remove 11,558 + GFF828 0.49 -0.6 11,559 - GFF828 0.49 +2.0 11,652 + GFF828 0.59 +0.9 11,731 + GFF828 0.67 -0.1 11,739 + GFF828 0.67 +0.7 11,869 - GFF828 0.80 -0.7 12,131 + -0.1 12,133 + -0.7 12,134 - +0.1 12,317 - GFF829 0.87 -0.5 12,390 - +0.7 13,791 + +0.4 13,871 - +0.2 13,871 - +0.4 13,909 - -0.5 13,911 - +0.7 13,911 - -1.0 13,981 + GFF831 0.19 -0.2 13,982 - GFF831 0.19 -0.6 13,996 - GFF831 0.22 -0.9 13,996 - GFF831 0.22 -0.6 14,052 + GFF831 0.34 -1.5 14,223 - GFF831 0.69 -0.4 14,313 + GFF831 0.87 +0.3 14,548 - -0.3 14,638 + +0.3 14,708 + GFF832 0.12 +1.0 14,738 - GFF832 0.13 +0.9
Or see this region's nucleotide sequence