Experiment: LB_plus_SM_buffer with EIP16_phage 0.875 MOI
Only strains with sufficient reads to estimate fitness are shown,
but the strain fitness values are still rather noisy and may be biased towards zero.
Strains near the edge of a gene are not shown as being associated with that
gene (they are in grey).
Strains in the central 10-90% of a gene are color coded by the insertion's strand.
Usually, "+" means that the selectable marker is encoded on the forward strand,
i.e., transcribed rightward.
500 nt GFF764 and GFF765 are separated by 29 nucleotides GFF765 and GFF766 are separated by 9 nucleotides GFF766 and GFF767 overlap by 4 nucleotides GFF767 and GFF768 are separated by 105 nucleotides
GFF764 - Ascorbate-specific PTS system, EIIC component, at 121,590 to 122,933
GFF764
GFF765 - Putative phosphotransferase enzyme II, B component, at 122,963 to 123,283
GFF765
GFF766 - Choline-sulfatase (EC 3.1.6.6), at 123,293 to 124,780
GFF766
GFF767 - hypothetical protein, at 124,777 to 124,893
GFF767
GFF768 - Arginine ABC transporter, periplasmic arginine-binding protein ArtJ, at 124,999 to 125,730
GFF768
Position (kb) 123
124
125 Strain fitness (log2 ratio) -2
-1
0
1
2 at 122.372 kb on - strand, within GFF764 at 122.400 kb on + strand, within GFF764 at 122.406 kb on + strand, within GFF764 at 122.407 kb on - strand, within GFF764 at 122.439 kb on + strand, within GFF764 at 122.439 kb on + strand, within GFF764 at 122.439 kb on + strand, within GFF764 at 122.439 kb on + strand, within GFF764 at 123.329 kb on - strand at 123.415 kb on + strand at 123.416 kb on - strand at 123.419 kb on + strand at 123.419 kb on + strand at 123.439 kb on + strand at 123.440 kb on - strand at 123.440 kb on - strand at 123.555 kb on - strand, within GFF766 at 123.565 kb on + strand, within GFF766 at 123.622 kb on + strand, within GFF766 at 123.636 kb on + strand, within GFF766 at 123.774 kb on - strand, within GFF766 at 123.832 kb on + strand, within GFF766 at 123.864 kb on + strand, within GFF766 at 123.929 kb on + strand, within GFF766 at 123.957 kb on - strand, within GFF766 at 124.007 kb on - strand, within GFF766 at 124.060 kb on + strand, within GFF766 at 124.061 kb on - strand, within GFF766 at 124.099 kb on + strand, within GFF766 at 124.181 kb on - strand, within GFF766 at 124.196 kb on - strand, within GFF766 at 124.424 kb on + strand, within GFF766 at 124.431 kb on - strand, within GFF766 at 124.431 kb on - strand, within GFF766 at 124.475 kb on + strand, within GFF766 at 124.561 kb on + strand, within GFF766 at 124.669 kb on + strand at 124.707 kb on + strand at 124.707 kb on + strand at 124.707 kb on + strand at 124.708 kb on - strand at 124.708 kb on - strand at 124.798 kb on - strand, within GFF767 at 124.831 kb on - strand, within GFF767 at 124.957 kb on + strand at 124.957 kb on + strand at 124.958 kb on - strand at 124.960 kb on - strand at 124.993 kb on + strand at 124.994 kb on - strand at 124.994 kb on - strand at 125.091 kb on + strand, within GFF768 at 125.133 kb on - strand, within GFF768 at 125.229 kb on + strand, within GFF768 at 125.360 kb on - strand, within GFF768 at 125.420 kb on + strand, within GFF768 at 125.430 kb on + strand, within GFF768 at 125.514 kb on + strand, within GFF768 at 125.544 kb on + strand, within GFF768 at 125.570 kb on - strand, within GFF768 at 125.757 kb on - strand
Per-strain Table
Position Strand Gene LocusTag Fraction LB_plus_SM_buffer with EIP16_phage 0.875 MOI remove 122,372 - GFF764 0.58 +0.3 122,400 + GFF764 0.60 +0.3 122,406 + GFF764 0.61 +0.2 122,407 - GFF764 0.61 -1.1 122,439 + GFF764 0.63 +0.2 122,439 + GFF764 0.63 -0.5 122,439 + GFF764 0.63 -0.0 122,439 + GFF764 0.63 -0.3 123,329 - +2.4 123,415 + +0.1 123,416 - -0.3 123,419 + -0.4 123,419 + -0.1 123,439 + +1.1 123,440 - -0.4 123,440 - -0.1 123,555 - GFF766 0.18 -0.2 123,565 + GFF766 0.18 +1.8 123,622 + GFF766 0.22 +2.0 123,636 + GFF766 0.23 -0.6 123,774 - GFF766 0.32 +0.5 123,832 + GFF766 0.36 +0.1 123,864 + GFF766 0.38 +0.2 123,929 + GFF766 0.43 +0.0 123,957 - GFF766 0.45 -0.0 124,007 - GFF766 0.48 -0.5 124,060 + GFF766 0.52 +0.8 124,061 - GFF766 0.52 +0.6 124,099 + GFF766 0.54 -0.5 124,181 - GFF766 0.60 +0.2 124,196 - GFF766 0.61 -0.8 124,424 + GFF766 0.76 +1.0 124,431 - GFF766 0.76 -0.0 124,431 - GFF766 0.76 +1.1 124,475 + GFF766 0.79 -0.1 124,561 + GFF766 0.85 -0.3 124,669 + +0.5 124,707 + +0.4 124,707 + -0.7 124,707 + -0.8 124,708 - +0.1 124,708 - +0.7 124,798 - GFF767 0.18 -0.1 124,831 - GFF767 0.46 -0.2 124,957 + -0.3 124,957 + -0.7 124,958 - +0.2 124,960 - +0.0 124,993 + -0.4 124,994 - -0.1 124,994 - -0.7 125,091 + GFF768 0.13 +0.0 125,133 - GFF768 0.18 +1.2 125,229 + GFF768 0.31 -2.2 125,360 - GFF768 0.49 +0.3 125,420 + GFF768 0.58 +0.6 125,430 + GFF768 0.59 +1.5 125,514 + GFF768 0.70 +0.3 125,544 + GFF768 0.74 -0.4 125,570 - GFF768 0.78 +0.6 125,757 - -0.4
Or see this region's nucleotide sequence