Experiment: LB_plus_SM_buffer with EIP16_phage 0.875 MOI
Only strains with sufficient reads to estimate fitness are shown,
but the strain fitness values are still rather noisy and may be biased towards zero.
Strains near the edge of a gene are not shown as being associated with that
gene (they are in grey).
Strains in the central 10-90% of a gene are color coded by the insertion's strand.
Usually, "+" means that the selectable marker is encoded on the forward strand,
i.e., transcribed rightward.
500 nt GFF593 and GFF594 are separated by 32 nucleotides GFF594 and GFF595 are separated by 344 nucleotides GFF595 and GFF596 are separated by 3 nucleotides
GFF593 - Putative ribonucleoprotein related-protein, at 328,401 to 329,954
GFF593
GFF594 - Putative ribonucleoprotein related-protein, at 329,987 to 330,154
GFF594
GFF595 - 'RNA-2',3'-PO4:RNA-5'-OH ligase' transl_table=11, at 330,499 to 331,716
GFF595
GFF596 - 'RNA 3'-terminal phosphate cyclase (EC 6.5.1.4)' transl_table=11, at 331,720 to 332,739
GFF596
Position (kb) 330
331
332 Strain fitness (log2 ratio) -1
0
1
2 at 329.642 kb on + strand, within GFF593 at 329.643 kb on - strand, within GFF593 at 329.682 kb on - strand, within GFF593 at 329.682 kb on - strand, within GFF593 at 329.711 kb on + strand, within GFF593 at 329.808 kb on + strand at 329.840 kb on + strand at 329.841 kb on - strand at 329.876 kb on + strand at 329.884 kb on + strand at 329.884 kb on + strand at 329.885 kb on - strand at 329.888 kb on + strand at 329.908 kb on - strand at 329.953 kb on - strand at 329.953 kb on - strand at 329.953 kb on - strand at 329.953 kb on - strand at 329.959 kb on + strand at 329.960 kb on - strand at 330.152 kb on + strand at 330.209 kb on - strand at 330.266 kb on - strand at 330.275 kb on - strand at 330.348 kb on - strand at 330.389 kb on + strand at 330.389 kb on + strand at 330.460 kb on + strand at 330.515 kb on + strand at 330.515 kb on + strand at 330.809 kb on + strand, within GFF595 at 330.966 kb on + strand, within GFF595 at 330.970 kb on + strand, within GFF595 at 330.972 kb on + strand, within GFF595 at 331.093 kb on + strand, within GFF595 at 331.094 kb on - strand, within GFF595 at 331.163 kb on - strand, within GFF595 at 331.183 kb on + strand, within GFF595 at 331.336 kb on + strand, within GFF595 at 331.336 kb on + strand, within GFF595 at 331.336 kb on + strand, within GFF595 at 331.336 kb on + strand, within GFF595 at 331.337 kb on - strand, within GFF595 at 331.337 kb on - strand, within GFF595 at 331.372 kb on + strand, within GFF595 at 331.373 kb on - strand, within GFF595 at 331.373 kb on - strand, within GFF595 at 331.373 kb on - strand, within GFF595 at 331.629 kb on + strand at 331.681 kb on + strand at 331.681 kb on + strand at 331.682 kb on - strand at 331.982 kb on - strand, within GFF596 at 332.656 kb on - strand
Per-strain Table
Position Strand Gene LocusTag Fraction LB_plus_SM_buffer with EIP16_phage 0.875 MOI remove 329,642 + GFF593 0.80 +0.3 329,643 - GFF593 0.80 +0.8 329,682 - GFF593 0.82 +0.2 329,682 - GFF593 0.82 +0.2 329,711 + GFF593 0.84 -0.3 329,808 + +0.6 329,840 + -1.0 329,841 - +0.1 329,876 + +0.6 329,884 + -0.2 329,884 + -0.2 329,885 - +0.0 329,888 + +0.1 329,908 - +0.3 329,953 - +0.3 329,953 - -0.1 329,953 - -0.6 329,953 - -0.1 329,959 + +0.0 329,960 - +0.3 330,152 + -0.5 330,209 - +0.8 330,266 - +0.9 330,275 - +0.1 330,348 - +0.9 330,389 + -0.5 330,389 + -0.3 330,460 + +0.2 330,515 + -0.1 330,515 + +0.3 330,809 + GFF595 0.25 +0.2 330,966 + GFF595 0.38 +0.3 330,970 + GFF595 0.39 +2.2 330,972 + GFF595 0.39 +1.0 331,093 + GFF595 0.49 -0.4 331,094 - GFF595 0.49 +2.0 331,163 - GFF595 0.55 +0.4 331,183 + GFF595 0.56 -0.1 331,336 + GFF595 0.69 -0.0 331,336 + GFF595 0.69 +0.3 331,336 + GFF595 0.69 +1.8 331,336 + GFF595 0.69 +1.3 331,337 - GFF595 0.69 +0.4 331,337 - GFF595 0.69 -0.1 331,372 + GFF595 0.72 -0.2 331,373 - GFF595 0.72 +0.2 331,373 - GFF595 0.72 +0.2 331,373 - GFF595 0.72 -0.0 331,629 + -0.0 331,681 + -0.3 331,681 + +0.4 331,682 - +0.2 331,982 - GFF596 0.26 +0.2 332,656 - +0.0
Or see this region's nucleotide sequence