Experiment: LB_plus_SM_buffer with EIP16_phage 0.875 MOI
Only strains with sufficient reads to estimate fitness are shown,
but the strain fitness values are still rather noisy and may be biased towards zero.
Strains near the edge of a gene are not shown as being associated with that
gene (they are in grey).
Strains in the central 10-90% of a gene are color coded by the insertion's strand.
Usually, "+" means that the selectable marker is encoded on the forward strand,
i.e., transcribed rightward.
500 nt GFF583 and GFF584 are separated by 337 nucleotides GFF584 and GFF585 are separated by 192 nucleotides
GFF583 - Nitrate/nitrite transporter, at 317,804 to 319,138
GFF583
GFF584 - Glycerol dehydrogenase (EC 1.1.1.6), at 319,476 to 320,585
GFF584
GFF585 - Phosphate ABC transporter, periplasmic phosphate-binding protein PstS (TC 3.A.1.7.1), at 320,778 to 322,238
GFF585
Position (kb) 319
320
321 Strain fitness (log2 ratio) -1
0
1
2
3 at 318.614 kb on - strand, within GFF583 at 318.634 kb on - strand, within GFF583 at 318.643 kb on + strand, within GFF583 at 318.673 kb on + strand, within GFF583 at 318.674 kb on - strand, within GFF583 at 318.768 kb on + strand, within GFF583 at 318.940 kb on + strand, within GFF583 at 318.941 kb on - strand, within GFF583 at 318.941 kb on - strand, within GFF583 at 318.942 kb on + strand, within GFF583 at 318.950 kb on - strand, within GFF583 at 319.049 kb on - strand at 319.049 kb on - strand at 319.050 kb on + strand at 319.050 kb on + strand at 319.087 kb on + strand at 319.302 kb on + strand at 319.481 kb on + strand at 319.820 kb on + strand, within GFF584 at 319.862 kb on + strand, within GFF584 at 319.867 kb on - strand, within GFF584 at 319.893 kb on + strand, within GFF584 at 319.911 kb on - strand, within GFF584 at 319.940 kb on + strand, within GFF584 at 319.941 kb on - strand, within GFF584 at 319.941 kb on - strand, within GFF584 at 319.941 kb on - strand, within GFF584 at 320.094 kb on + strand, within GFF584 at 320.105 kb on - strand, within GFF584 at 320.105 kb on - strand, within GFF584 at 320.112 kb on + strand, within GFF584 at 320.187 kb on - strand, within GFF584 at 320.187 kb on - strand, within GFF584 at 320.192 kb on - strand, within GFF584 at 320.280 kb on + strand, within GFF584 at 320.324 kb on + strand, within GFF584 at 320.325 kb on - strand, within GFF584 at 320.325 kb on - strand, within GFF584 at 320.383 kb on + strand, within GFF584 at 320.383 kb on + strand, within GFF584 at 320.705 kb on + strand at 320.707 kb on + strand at 320.708 kb on - strand at 320.776 kb on + strand at 320.813 kb on + strand at 320.814 kb on - strand at 320.844 kb on + strand at 320.844 kb on + strand at 321.012 kb on - strand, within GFF585 at 321.012 kb on - strand, within GFF585 at 321.068 kb on + strand, within GFF585 at 321.069 kb on - strand, within GFF585 at 321.090 kb on - strand, within GFF585 at 321.124 kb on + strand, within GFF585 at 321.137 kb on - strand, within GFF585 at 321.213 kb on + strand, within GFF585 at 321.214 kb on - strand, within GFF585 at 321.313 kb on - strand, within GFF585 at 321.328 kb on - strand, within GFF585 at 321.364 kb on - strand, within GFF585 at 321.549 kb on + strand, within GFF585
Per-strain Table
Position Strand Gene LocusTag Fraction LB_plus_SM_buffer with EIP16_phage 0.875 MOI remove 318,614 - GFF583 0.61 +0.6 318,634 - GFF583 0.62 -0.4 318,643 + GFF583 0.63 -0.4 318,673 + GFF583 0.65 -0.6 318,674 - GFF583 0.65 +0.0 318,768 + GFF583 0.72 +0.8 318,940 + GFF583 0.85 -0.7 318,941 - GFF583 0.85 -0.1 318,941 - GFF583 0.85 +0.1 318,942 + GFF583 0.85 -0.4 318,950 - GFF583 0.86 +0.3 319,049 - +0.4 319,049 - +0.9 319,050 + +0.0 319,050 + +0.9 319,087 + +0.5 319,302 + +0.8 319,481 + +0.1 319,820 + GFF584 0.31 +0.4 319,862 + GFF584 0.35 +0.1 319,867 - GFF584 0.35 +0.0 319,893 + GFF584 0.38 +0.1 319,911 - GFF584 0.39 -0.1 319,940 + GFF584 0.42 +1.0 319,941 - GFF584 0.42 +0.4 319,941 - GFF584 0.42 -1.4 319,941 - GFF584 0.42 -1.2 320,094 + GFF584 0.56 +0.9 320,105 - GFF584 0.57 -0.1 320,105 - GFF584 0.57 +0.0 320,112 + GFF584 0.57 -0.9 320,187 - GFF584 0.64 -0.1 320,187 - GFF584 0.64 -0.1 320,192 - GFF584 0.65 +0.3 320,280 + GFF584 0.72 +1.2 320,324 + GFF584 0.76 +0.7 320,325 - GFF584 0.76 +0.4 320,325 - GFF584 0.76 +0.7 320,383 + GFF584 0.82 +0.5 320,383 + GFF584 0.82 +1.6 320,705 + -1.1 320,707 + +0.3 320,708 - -0.3 320,776 + +0.9 320,813 + +0.2 320,814 - +0.3 320,844 + +0.1 320,844 + -0.1 321,012 - GFF585 0.16 +0.3 321,012 - GFF585 0.16 -0.2 321,068 + GFF585 0.20 -0.2 321,069 - GFF585 0.20 +1.1 321,090 - GFF585 0.21 +3.0 321,124 + GFF585 0.24 +1.8 321,137 - GFF585 0.25 +0.5 321,213 + GFF585 0.30 -1.7 321,214 - GFF585 0.30 -0.1 321,313 - GFF585 0.37 +0.2 321,328 - GFF585 0.38 +0.0 321,364 - GFF585 0.40 +0.9 321,549 + GFF585 0.53 +0.2
Or see this region's nucleotide sequence