Experiment: LB_plus_SM_buffer with EIP16_phage 0.875 MOI
Only strains with sufficient reads to estimate fitness are shown,
but the strain fitness values are still rather noisy and may be biased towards zero.
Strains near the edge of a gene are not shown as being associated with that
gene (they are in grey).
Strains in the central 10-90% of a gene are color coded by the insertion's strand.
Usually, "+" means that the selectable marker is encoded on the forward strand,
i.e., transcribed rightward.
500 nt GFF4849 and GFF4850 are separated by 112 nucleotides GFF4850 and GFF4851 overlap by 4 nucleotides GFF4851 and GFF4852 are separated by 448 nucleotides
GFF4849 - NADPH dependent preQ0 reductase (EC 1.7.1.13), at 147,847 to 148,695
GFF4849
GFF4850 - Decarboxylase family protein, at 148,808 to 150,172
GFF4850
GFF4851 - hypothetical protein, at 150,169 to 150,288
GFF4851
GFF4852 - Serine transporter, at 150,737 to 152,026
GFF4852
Position (kb) 148
149
150
151 Strain fitness (log2 ratio) -1
0
1
2
3 at 147.819 kb on + strand at 147.824 kb on + strand at 148.024 kb on + strand, within GFF4849 at 148.101 kb on + strand, within GFF4849 at 148.325 kb on - strand, within GFF4849 at 148.376 kb on + strand, within GFF4849 at 148.522 kb on - strand, within GFF4849 at 148.544 kb on + strand, within GFF4849 at 148.610 kb on - strand, within GFF4849 at 148.731 kb on + strand at 148.731 kb on + strand at 148.732 kb on - strand at 148.761 kb on - strand at 148.765 kb on + strand at 148.796 kb on + strand at 148.813 kb on + strand at 148.819 kb on + strand at 148.819 kb on + strand at 148.844 kb on - strand at 148.844 kb on - strand at 148.934 kb on - strand at 148.996 kb on + strand, within GFF4850 at 149.001 kb on + strand, within GFF4850 at 149.334 kb on + strand, within GFF4850 at 149.561 kb on - strand, within GFF4850 at 149.606 kb on + strand, within GFF4850 at 149.722 kb on + strand, within GFF4850 at 149.722 kb on + strand, within GFF4850 at 149.854 kb on + strand, within GFF4850 at 149.915 kb on - strand, within GFF4850 at 150.143 kb on + strand at 150.143 kb on + strand at 150.143 kb on + strand at 150.143 kb on + strand at 150.245 kb on - strand, within GFF4851 at 150.462 kb on + strand at 150.463 kb on - strand at 150.517 kb on - strand at 150.522 kb on - strand at 150.543 kb on + strand at 150.760 kb on + strand at 150.761 kb on - strand at 151.039 kb on + strand, within GFF4852 at 151.050 kb on - strand, within GFF4852 at 151.100 kb on - strand, within GFF4852
Per-strain Table
Position Strand Gene LocusTag Fraction LB_plus_SM_buffer with EIP16_phage 0.875 MOI remove 147,819 + +0.6 147,824 + +0.3 148,024 + GFF4849 0.21 -0.0 148,101 + GFF4849 0.30 +0.4 148,325 - GFF4849 0.56 +0.4 148,376 + GFF4849 0.62 -1.3 148,522 - GFF4849 0.80 +1.0 148,544 + GFF4849 0.82 -0.7 148,610 - GFF4849 0.90 +0.4 148,731 + -0.5 148,731 + -0.6 148,732 - -1.0 148,761 - +0.3 148,765 + +0.4 148,796 + -0.1 148,813 + -0.2 148,819 + +0.2 148,819 + +0.6 148,844 - +0.3 148,844 - +0.6 148,934 - +0.1 148,996 + GFF4850 0.14 +0.3 149,001 + GFF4850 0.14 -0.5 149,334 + GFF4850 0.39 +0.6 149,561 - GFF4850 0.55 +3.0 149,606 + GFF4850 0.58 -0.1 149,722 + GFF4850 0.67 -0.6 149,722 + GFF4850 0.67 +0.3 149,854 + GFF4850 0.77 -0.5 149,915 - GFF4850 0.81 +0.4 150,143 + +0.2 150,143 + +0.5 150,143 + -0.6 150,143 + +0.1 150,245 - GFF4851 0.63 -0.3 150,462 + -0.1 150,463 - -0.2 150,517 - -0.4 150,522 - +0.4 150,543 + -0.1 150,760 + +1.2 150,761 - +1.8 151,039 + GFF4852 0.23 +0.8 151,050 - GFF4852 0.24 -0.6 151,100 - GFF4852 0.28 +0.5
Or see this region's nucleotide sequence