Experiment: LB_plus_SM_buffer with EIP16_phage 0.875 MOI
Only strains with sufficient reads to estimate fitness are shown,
but the strain fitness values are still rather noisy and may be biased towards zero.
Strains near the edge of a gene are not shown as being associated with that
gene (they are in grey).
Strains in the central 10-90% of a gene are color coded by the insertion's strand.
Usually, "+" means that the selectable marker is encoded on the forward strand,
i.e., transcribed rightward.
500 nt GFF455 and GFF456 are separated by 157 nucleotides GFF456 and GFF457 are separated by 149 nucleotides GFF457 and GFF458 are separated by 49 nucleotides
GFF455 - FIG00638451: hypothetical protein, at 176,928 to 177,218
GFF455
GFF456 - hypothetical protein, at 177,376 to 177,507
GFF456
GFF457 - probable exported protein YPO4070, at 177,657 to 178,367
GFF457
GFF458 - 2-ketoaldonate reductase, broad specificity (EC 1.1.1.215) (EC 1.1.1.-), at 178,417 to 179,391
GFF458
Position (kb) 177
178
179 Strain fitness (log2 ratio) -1
0
1
2 at 176.768 kb on - strand at 176.848 kb on + strand at 176.894 kb on - strand at 176.949 kb on + strand at 176.950 kb on - strand at 177.097 kb on - strand, within GFF455 at 177.209 kb on + strand at 177.391 kb on - strand, within GFF456 at 177.434 kb on + strand, within GFF456 at 177.529 kb on + strand at 177.653 kb on - strand at 177.710 kb on - strand at 177.710 kb on - strand at 177.710 kb on - strand at 177.719 kb on + strand at 177.864 kb on + strand, within GFF457 at 177.876 kb on + strand, within GFF457 at 177.876 kb on + strand, within GFF457 at 177.876 kb on + strand, within GFF457 at 177.876 kb on + strand, within GFF457 at 177.877 kb on - strand, within GFF457 at 177.877 kb on - strand, within GFF457 at 177.967 kb on - strand, within GFF457 at 178.206 kb on + strand, within GFF457 at 178.206 kb on + strand, within GFF457 at 178.284 kb on + strand, within GFF457 at 178.284 kb on + strand, within GFF457 at 178.329 kb on - strand at 178.352 kb on - strand at 178.483 kb on - strand at 178.509 kb on - strand at 178.558 kb on - strand, within GFF458 at 178.628 kb on - strand, within GFF458 at 178.628 kb on - strand, within GFF458 at 178.695 kb on + strand, within GFF458 at 178.714 kb on - strand, within GFF458 at 178.806 kb on + strand, within GFF458 at 178.806 kb on + strand, within GFF458 at 178.821 kb on - strand, within GFF458 at 178.886 kb on + strand, within GFF458 at 178.888 kb on - strand, within GFF458 at 179.136 kb on + strand, within GFF458 at 179.157 kb on + strand, within GFF458
Per-strain Table
Position Strand Gene LocusTag Fraction LB_plus_SM_buffer with EIP16_phage 0.875 MOI remove 176,768 - +0.1 176,848 + -0.7 176,894 - -0.3 176,949 + +0.6 176,950 - -0.6 177,097 - GFF455 0.58 -0.3 177,209 + +0.7 177,391 - GFF456 0.11 -0.2 177,434 + GFF456 0.44 -0.1 177,529 + +0.4 177,653 - +0.1 177,710 - -0.3 177,710 - +0.4 177,710 - +0.5 177,719 + +0.3 177,864 + GFF457 0.29 +0.7 177,876 + GFF457 0.31 +0.2 177,876 + GFF457 0.31 -0.1 177,876 + GFF457 0.31 -0.1 177,876 + GFF457 0.31 +0.1 177,877 - GFF457 0.31 +0.0 177,877 - GFF457 0.31 -0.2 177,967 - GFF457 0.44 -0.2 178,206 + GFF457 0.77 +1.6 178,206 + GFF457 0.77 -0.3 178,284 + GFF457 0.88 +0.9 178,284 + GFF457 0.88 +0.1 178,329 - -0.1 178,352 - +0.5 178,483 - -0.2 178,509 - -0.2 178,558 - GFF458 0.14 +0.9 178,628 - GFF458 0.22 +2.1 178,628 - GFF458 0.22 -0.1 178,695 + GFF458 0.29 -0.5 178,714 - GFF458 0.30 -0.0 178,806 + GFF458 0.40 -0.9 178,806 + GFF458 0.40 -0.5 178,821 - GFF458 0.41 -0.5 178,886 + GFF458 0.48 +0.1 178,888 - GFF458 0.48 +0.1 179,136 + GFF458 0.74 +0.4 179,157 + GFF458 0.76 +0.1
Or see this region's nucleotide sequence