Experiment: LB_plus_SM_buffer with EIP16_phage 0.875 MOI
Only strains with sufficient reads to estimate fitness are shown,
but the strain fitness values are still rather noisy and may be biased towards zero.
Strains near the edge of a gene are not shown as being associated with that
gene (they are in grey).
Strains in the central 10-90% of a gene are color coded by the insertion's strand.
Usually, "+" means that the selectable marker is encoded on the forward strand,
i.e., transcribed rightward.
500 nt GFF4551 and GFF4552 are separated by 96 nucleotides GFF4552 and GFF4553 overlap by 26 nucleotides GFF4553 and GFF4554 overlap by 8 nucleotides
GFF4551 - 'Alpha-ribazole-5'-phosphate phosphatase (EC 3.1.3.73)' transl_table=11, at 335,286 to 335,894
GFF4551
GFF4552 - L-threonine 3-O-phosphate decarboxylase (EC 4.1.1.81), at 335,991 to 337,085
GFF4552
GFF4553 - Nicotinate-nucleotide adenylyltransferase (EC 2.7.7.18), at 337,060 to 337,710
GFF4553
GFF4554 - DNA polymerase III delta subunit (EC 2.7.7.7), at 337,703 to 338,725
GFF4554
Position (kb) 335
336
337
338 Strain fitness (log2 ratio) -1
0
1 at 335.066 kb on - strand at 335.069 kb on - strand at 335.099 kb on - strand at 335.312 kb on + strand at 335.316 kb on + strand at 335.346 kb on + strand at 335.346 kb on + strand at 335.347 kb on - strand, within GFF4551 at 335.347 kb on - strand, within GFF4551 at 335.347 kb on - strand, within GFF4551 at 335.480 kb on + strand, within GFF4551 at 335.647 kb on + strand, within GFF4551 at 335.648 kb on - strand, within GFF4551 at 335.658 kb on - strand, within GFF4551 at 335.777 kb on + strand, within GFF4551 at 335.778 kb on - strand, within GFF4551 at 335.778 kb on - strand, within GFF4551 at 335.778 kb on - strand, within GFF4551 at 335.778 kb on - strand, within GFF4551 at 335.839 kb on + strand at 335.898 kb on - strand at 335.898 kb on - strand at 335.989 kb on + strand at 336.068 kb on - strand at 336.226 kb on + strand, within GFF4552 at 336.449 kb on + strand, within GFF4552 at 336.563 kb on + strand, within GFF4552 at 336.585 kb on - strand, within GFF4552 at 336.885 kb on + strand, within GFF4552 at 336.885 kb on + strand, within GFF4552 at 336.959 kb on + strand, within GFF4552 at 336.973 kb on - strand, within GFF4552 at 336.996 kb on + strand
Per-strain Table
Position Strand Gene LocusTag Fraction LB_plus_SM_buffer with EIP16_phage 0.875 MOI remove 335,066 - +0.9 335,069 - +0.1 335,099 - +0.6 335,312 + +0.2 335,316 + -0.4 335,346 + +0.4 335,346 + -0.2 335,347 - GFF4551 0.10 -0.3 335,347 - GFF4551 0.10 -0.5 335,347 - GFF4551 0.10 +0.3 335,480 + GFF4551 0.32 +0.2 335,647 + GFF4551 0.59 -0.1 335,648 - GFF4551 0.59 -0.5 335,658 - GFF4551 0.61 +0.2 335,777 + GFF4551 0.81 -0.5 335,778 - GFF4551 0.81 +0.2 335,778 - GFF4551 0.81 -0.6 335,778 - GFF4551 0.81 -0.0 335,778 - GFF4551 0.81 -0.6 335,839 + +0.6 335,898 - -0.2 335,898 - -0.1 335,989 + +0.4 336,068 - -0.5 336,226 + GFF4552 0.21 +0.4 336,449 + GFF4552 0.42 +0.6 336,563 + GFF4552 0.52 +0.0 336,585 - GFF4552 0.54 -0.3 336,885 + GFF4552 0.82 -0.5 336,885 + GFF4552 0.82 +0.1 336,959 + GFF4552 0.88 +0.0 336,973 - GFF4552 0.90 -0.1 336,996 + -0.6
Or see this region's nucleotide sequence