Experiment: LB_plus_SM_buffer with EIP16_phage 0.875 MOI
Only strains with sufficient reads to estimate fitness are shown,
but the strain fitness values are still rather noisy and may be biased towards zero.
Strains near the edge of a gene are not shown as being associated with that
gene (they are in grey).
Strains in the central 10-90% of a gene are color coded by the insertion's strand.
Usually, "+" means that the selectable marker is encoded on the forward strand,
i.e., transcribed rightward.
500 nt GFF4532 and GFF4533 overlap by 32 nucleotides GFF4533 and GFF4534 are separated by 53 nucleotides
GFF4532 - Sensor kinase CitA, DpiB (EC 2.7.3.-), at 318,241 to 319,902
GFF4532
GFF4533 - Transcriptional regulatory protein CitB, DpiA, at 319,871 to 320,551
GFF4533
GFF4534 - C4-dicarboxylate transporter DcuC (TC 2.A.61.1.1), at 320,605 to 321,948
GFF4534
Position (kb) 319
320
321 Strain fitness (log2 ratio) -1
0
1
2 at 318.925 kb on - strand, within GFF4532 at 318.970 kb on - strand, within GFF4532 at 319.274 kb on + strand, within GFF4532 at 319.333 kb on + strand, within GFF4532 at 319.333 kb on + strand, within GFF4532 at 319.387 kb on - strand, within GFF4532 at 319.392 kb on + strand, within GFF4532 at 319.502 kb on + strand, within GFF4532 at 319.502 kb on + strand, within GFF4532 at 319.502 kb on + strand, within GFF4532 at 319.649 kb on + strand, within GFF4532 at 319.718 kb on + strand, within GFF4532 at 319.718 kb on + strand, within GFF4532 at 319.719 kb on - strand, within GFF4532 at 319.719 kb on - strand, within GFF4532 at 319.719 kb on - strand, within GFF4532 at 319.719 kb on - strand, within GFF4532 at 319.719 kb on - strand, within GFF4532 at 319.891 kb on - strand at 319.938 kb on - strand at 320.029 kb on - strand, within GFF4533 at 320.041 kb on + strand, within GFF4533 at 320.045 kb on + strand, within GFF4533 at 320.195 kb on - strand, within GFF4533 at 320.286 kb on + strand, within GFF4533 at 320.286 kb on + strand, within GFF4533 at 320.503 kb on + strand at 320.533 kb on + strand at 320.533 kb on + strand at 320.816 kb on + strand, within GFF4534 at 320.850 kb on + strand, within GFF4534 at 320.929 kb on + strand, within GFF4534 at 320.946 kb on + strand, within GFF4534 at 320.947 kb on - strand, within GFF4534 at 321.177 kb on + strand, within GFF4534 at 321.204 kb on + strand, within GFF4534 at 321.204 kb on + strand, within GFF4534 at 321.322 kb on - strand, within GFF4534
Per-strain Table
Position Strand Gene LocusTag Fraction LB_plus_SM_buffer with EIP16_phage 0.875 MOI remove 318,925 - GFF4532 0.41 -0.9 318,970 - GFF4532 0.44 -0.1 319,274 + GFF4532 0.62 -1.1 319,333 + GFF4532 0.66 +0.4 319,333 + GFF4532 0.66 +0.6 319,387 - GFF4532 0.69 +0.1 319,392 + GFF4532 0.69 -0.6 319,502 + GFF4532 0.76 -0.7 319,502 + GFF4532 0.76 -0.5 319,502 + GFF4532 0.76 +0.1 319,649 + GFF4532 0.85 +2.4 319,718 + GFF4532 0.89 +0.8 319,718 + GFF4532 0.89 +0.0 319,719 - GFF4532 0.89 +0.2 319,719 - GFF4532 0.89 +0.1 319,719 - GFF4532 0.89 +0.7 319,719 - GFF4532 0.89 -0.1 319,719 - GFF4532 0.89 -0.0 319,891 - -0.1 319,938 - +0.2 320,029 - GFF4533 0.23 +0.4 320,041 + GFF4533 0.25 +0.1 320,045 + GFF4533 0.26 -0.2 320,195 - GFF4533 0.48 +0.6 320,286 + GFF4533 0.61 +0.5 320,286 + GFF4533 0.61 +0.3 320,503 + -0.4 320,533 + -0.1 320,533 + +1.8 320,816 + GFF4534 0.16 -0.3 320,850 + GFF4534 0.18 +1.6 320,929 + GFF4534 0.24 -0.0 320,946 + GFF4534 0.25 -0.3 320,947 - GFF4534 0.25 -0.6 321,177 + GFF4534 0.43 -0.0 321,204 + GFF4534 0.45 -0.3 321,204 + GFF4534 0.45 -0.3 321,322 - GFF4534 0.53 +0.2
Or see this region's nucleotide sequence