Experiment: LB_plus_SM_buffer with EIP16_phage 0.875 MOI
Only strains with sufficient reads to estimate fitness are shown,
but the strain fitness values are still rather noisy and may be biased towards zero.
Strains near the edge of a gene are not shown as being associated with that
gene (they are in grey).
Strains in the central 10-90% of a gene are color coded by the insertion's strand.
Usually, "+" means that the selectable marker is encoded on the forward strand,
i.e., transcribed rightward.
500 nt GFF4442 and GFF4443 are separated by 43 nucleotides GFF4443 and GFF4444 overlap by 4 nucleotides GFF4444 and GFF4445 overlap by 8 nucleotides
GFF4442 - Cysteinyl-tRNA synthetase (EC 6.1.1.16), at 229,349 to 230,734
GFF4442
GFF4443 - Putative outer membrane protein, at 230,778 to 231,602
GFF4443
GFF4444 - Putative inner membrane protein, at 231,599 to 232,036
GFF4444
GFF4445 - Putative membrane-bound metal-dependent hydrolases, at 232,029 to 232,574
GFF4445
Position (kb) 230
231
232 Strain fitness (log2 ratio) -2
-1
0
1 at 230.755 kb on - strand at 230.870 kb on + strand, within GFF4443 at 230.899 kb on - strand, within GFF4443 at 231.051 kb on - strand, within GFF4443 at 231.051 kb on - strand, within GFF4443 at 231.144 kb on + strand, within GFF4443 at 231.150 kb on - strand, within GFF4443 at 231.188 kb on + strand, within GFF4443 at 231.193 kb on + strand, within GFF4443 at 231.291 kb on + strand, within GFF4443 at 231.291 kb on + strand, within GFF4443 at 231.292 kb on - strand, within GFF4443 at 231.292 kb on - strand, within GFF4443 at 231.293 kb on + strand, within GFF4443 at 231.295 kb on + strand, within GFF4443 at 231.295 kb on + strand, within GFF4443 at 231.410 kb on + strand, within GFF4443 at 231.536 kb on + strand at 231.537 kb on - strand at 231.978 kb on + strand, within GFF4444 at 232.065 kb on + strand at 232.083 kb on + strand at 232.083 kb on + strand at 232.141 kb on + strand, within GFF4445 at 232.153 kb on + strand, within GFF4445 at 232.154 kb on - strand, within GFF4445 at 232.181 kb on + strand, within GFF4445 at 232.181 kb on + strand, within GFF4445 at 232.521 kb on + strand at 232.522 kb on - strand at 232.569 kb on - strand at 232.574 kb on + strand
Per-strain Table
Position Strand Gene LocusTag Fraction LB_plus_SM_buffer with EIP16_phage 0.875 MOI remove 230,755 - -0.1 230,870 + GFF4443 0.11 -0.2 230,899 - GFF4443 0.15 -0.1 231,051 - GFF4443 0.33 +0.2 231,051 - GFF4443 0.33 +0.7 231,144 + GFF4443 0.44 +0.2 231,150 - GFF4443 0.45 +0.1 231,188 + GFF4443 0.50 +0.6 231,193 + GFF4443 0.50 +0.5 231,291 + GFF4443 0.62 -0.2 231,291 + GFF4443 0.62 +0.6 231,292 - GFF4443 0.62 -0.1 231,292 - GFF4443 0.62 -0.3 231,293 + GFF4443 0.62 -0.3 231,295 + GFF4443 0.63 +0.5 231,295 + GFF4443 0.63 +0.6 231,410 + GFF4443 0.77 +0.2 231,536 + -0.2 231,537 - +0.2 231,978 + GFF4444 0.87 -0.2 232,065 + +0.4 232,083 + -0.4 232,083 + +0.1 232,141 + GFF4445 0.21 -0.5 232,153 + GFF4445 0.23 +0.0 232,154 - GFF4445 0.23 +0.1 232,181 + GFF4445 0.28 +0.4 232,181 + GFF4445 0.28 -1.3 232,521 + +0.3 232,522 - -1.0 232,569 - +0.3 232,574 + -2.0
Or see this region's nucleotide sequence