Experiment: LB_plus_SM_buffer with EIP16_phage 0.875 MOI
Only strains with sufficient reads to estimate fitness are shown,
but the strain fitness values are still rather noisy and may be biased towards zero.
Strains near the edge of a gene are not shown as being associated with that
gene (they are in grey).
Strains in the central 10-90% of a gene are color coded by the insertion's strand.
Usually, "+" means that the selectable marker is encoded on the forward strand,
i.e., transcribed rightward.
500 nt GFF4403 and GFF4404 are separated by 153 nucleotides GFF4404 and GFF4405 are separated by 109 nucleotides GFF4405 and GFF4406 are separated by 0 nucleotides GFF4406 and GFF4407 overlap by 4 nucleotides
GFF4403 - Probable secreted protein, at 187,178 to 188,005
GFF4403
GFF4404 - Lead, cadmium, zinc and mercury transporting ATPase (EC 3.6.3.3) (EC 3.6.3.5); Copper-translocating P-type ATPase (EC 3.6.3.4), at 188,159 to 190,660
GFF4404
GFF4405 - HTH-type transcriptional regulator cueR, at 190,770 to 191,186
GFF4405
GFF4406 - Putative activity regulator of membrane protease YbbK, at 191,187 to 191,639
GFF4406
GFF4407 - Putative stomatin/prohibitin-family membrane protease subunit YbbK, at 191,636 to 192,553
GFF4407
Position (kb) 188
189
190
191 Strain fitness (log2 ratio) -1
0
1 at 187.234 kb on + strand at 187.263 kb on - strand, within GFF4403 at 187.340 kb on + strand, within GFF4403 at 187.341 kb on - strand, within GFF4403 at 187.469 kb on + strand, within GFF4403 at 187.573 kb on - strand, within GFF4403 at 187.716 kb on - strand, within GFF4403 at 188.240 kb on + strand at 188.313 kb on + strand at 188.345 kb on - strand at 188.398 kb on + strand at 188.654 kb on + strand, within GFF4404 at 188.698 kb on + strand, within GFF4404 at 188.938 kb on + strand, within GFF4404 at 189.014 kb on + strand, within GFF4404 at 189.014 kb on + strand, within GFF4404 at 189.015 kb on - strand, within GFF4404 at 189.119 kb on - strand, within GFF4404 at 189.266 kb on - strand, within GFF4404 at 189.267 kb on + strand, within GFF4404 at 189.348 kb on - strand, within GFF4404 at 189.389 kb on - strand, within GFF4404 at 189.775 kb on + strand, within GFF4404 at 189.808 kb on - strand, within GFF4404 at 190.301 kb on + strand, within GFF4404 at 190.302 kb on - strand, within GFF4404 at 190.649 kb on - strand at 190.661 kb on - strand at 190.702 kb on + strand at 190.705 kb on + strand at 190.769 kb on + strand at 190.804 kb on + strand at 190.858 kb on + strand, within GFF4405 at 190.859 kb on - strand, within GFF4405 at 190.859 kb on - strand, within GFF4405 at 190.944 kb on - strand, within GFF4405 at 191.142 kb on - strand, within GFF4405 at 191.243 kb on + strand, within GFF4406 at 191.336 kb on + strand, within GFF4406
Per-strain Table
Position Strand Gene LocusTag Fraction LB_plus_SM_buffer with EIP16_phage 0.875 MOI remove 187,234 + +0.2 187,263 - GFF4403 0.10 -0.3 187,340 + GFF4403 0.20 -0.6 187,341 - GFF4403 0.20 +0.1 187,469 + GFF4403 0.35 +0.9 187,573 - GFF4403 0.48 +0.4 187,716 - GFF4403 0.65 +1.1 188,240 + -1.2 188,313 + +0.0 188,345 - -0.8 188,398 + +0.4 188,654 + GFF4404 0.20 -0.8 188,698 + GFF4404 0.22 -0.7 188,938 + GFF4404 0.31 -0.6 189,014 + GFF4404 0.34 -0.1 189,014 + GFF4404 0.34 -0.6 189,015 - GFF4404 0.34 +0.2 189,119 - GFF4404 0.38 -0.6 189,266 - GFF4404 0.44 +1.0 189,267 + GFF4404 0.44 +0.8 189,348 - GFF4404 0.48 -0.4 189,389 - GFF4404 0.49 +0.4 189,775 + GFF4404 0.65 +0.2 189,808 - GFF4404 0.66 -0.1 190,301 + GFF4404 0.86 -0.3 190,302 - GFF4404 0.86 +0.0 190,649 - +0.3 190,661 - +0.7 190,702 + +1.3 190,705 + +0.2 190,769 + -0.3 190,804 + -0.3 190,858 + GFF4405 0.21 +0.1 190,859 - GFF4405 0.21 -0.4 190,859 - GFF4405 0.21 +0.2 190,944 - GFF4405 0.42 +0.4 191,142 - GFF4405 0.89 +0.3 191,243 + GFF4406 0.12 -0.0 191,336 + GFF4406 0.33 -0.1
Or see this region's nucleotide sequence