Experiment: LB_plus_SM_buffer with EIP16_phage 0.875 MOI
Only strains with sufficient reads to estimate fitness are shown,
but the strain fitness values are still rather noisy and may be biased towards zero.
Strains near the edge of a gene are not shown as being associated with that
gene (they are in grey).
Strains in the central 10-90% of a gene are color coded by the insertion's strand.
Usually, "+" means that the selectable marker is encoded on the forward strand,
i.e., transcribed rightward.
500 nt GFF4327 and GFF4328 are separated by 43 nucleotides GFF4328 and GFF4329 are separated by 2 nucleotides GFF4329 and GFF4330 are separated by 2 nucleotides
GFF4327 - tRNA S(4)U 4-thiouridine synthase (former ThiI) / Rhodanese-like domain required for thiamine synthesis, at 108,080 to 109,528
GFF4327
GFF4328 - 2-aminoethylphosphonate ABC transporter permease protein II (TC 3.A.1.9.1), at 109,572 to 110,369
GFF4328
GFF4329 - 2-aminoethylphosphonate ABC transporter permease protein I (TC 3.A.1.9.1), at 110,372 to 111,232
GFF4329
GFF4330 - 2-aminoethylphosphonate ABC transporter ATP-binding protein (TC 3.A.1.9.1), at 111,235 to 112,344
GFF4330
Position (kb) 110
111
112 Strain fitness (log2 ratio) -3
-2
-1
0
1
2 at 109.388 kb on + strand at 109.426 kb on + strand at 109.440 kb on + strand at 109.440 kb on + strand at 109.440 kb on + strand at 109.481 kb on + strand at 109.549 kb on + strand at 109.574 kb on - strand at 109.574 kb on - strand at 109.691 kb on + strand, within GFF4328 at 109.697 kb on + strand, within GFF4328 at 110.063 kb on + strand, within GFF4328 at 110.064 kb on - strand, within GFF4328 at 110.064 kb on - strand, within GFF4328 at 110.317 kb on - strand at 110.351 kb on + strand at 110.374 kb on - strand at 110.470 kb on + strand, within GFF4329 at 110.473 kb on - strand, within GFF4329 at 110.500 kb on - strand, within GFF4329 at 110.854 kb on - strand, within GFF4329 at 110.899 kb on + strand, within GFF4329 at 110.900 kb on - strand, within GFF4329 at 110.948 kb on - strand, within GFF4329 at 111.191 kb on + strand at 111.238 kb on + strand at 111.238 kb on + strand at 111.239 kb on - strand at 111.632 kb on + strand, within GFF4330 at 111.632 kb on + strand, within GFF4330 at 111.633 kb on - strand, within GFF4330 at 111.679 kb on - strand, within GFF4330 at 111.731 kb on + strand, within GFF4330 at 111.786 kb on + strand, within GFF4330 at 111.787 kb on - strand, within GFF4330 at 111.787 kb on - strand, within GFF4330 at 111.789 kb on + strand, within GFF4330 at 111.789 kb on + strand, within GFF4330 at 111.969 kb on + strand, within GFF4330 at 111.969 kb on + strand, within GFF4330 at 112.082 kb on + strand, within GFF4330
Per-strain Table
Position Strand Gene LocusTag Fraction LB_plus_SM_buffer with EIP16_phage 0.875 MOI remove 109,388 + +0.1 109,426 + +1.5 109,440 + +0.4 109,440 + +0.6 109,440 + +1.7 109,481 + +0.7 109,549 + +0.0 109,574 - -0.3 109,574 - -0.9 109,691 + GFF4328 0.15 -0.2 109,697 + GFF4328 0.16 +0.2 110,063 + GFF4328 0.62 -0.8 110,064 - GFF4328 0.62 +2.0 110,064 - GFF4328 0.62 +0.0 110,317 - +0.1 110,351 + +0.1 110,374 - +0.5 110,470 + GFF4329 0.11 -0.4 110,473 - GFF4329 0.12 -0.3 110,500 - GFF4329 0.15 -1.6 110,854 - GFF4329 0.56 +0.0 110,899 + GFF4329 0.61 -1.1 110,900 - GFF4329 0.61 +0.3 110,948 - GFF4329 0.67 -0.0 111,191 + -0.3 111,238 + +1.3 111,238 + +0.6 111,239 - -0.4 111,632 + GFF4330 0.36 -0.0 111,632 + GFF4330 0.36 -3.2 111,633 - GFF4330 0.36 -0.6 111,679 - GFF4330 0.40 -0.5 111,731 + GFF4330 0.45 +0.0 111,786 + GFF4330 0.50 +0.7 111,787 - GFF4330 0.50 -0.4 111,787 - GFF4330 0.50 +1.1 111,789 + GFF4330 0.50 +0.1 111,789 + GFF4330 0.50 +0.3 111,969 + GFF4330 0.66 -0.3 111,969 + GFF4330 0.66 -0.3 112,082 + GFF4330 0.76 +0.8
Or see this region's nucleotide sequence