Experiment: LB_plus_SM_buffer with EIP16_phage 0.875 MOI
Only strains with sufficient reads to estimate fitness are shown,
but the strain fitness values are still rather noisy and may be biased towards zero.
Strains near the edge of a gene are not shown as being associated with that
gene (they are in grey).
Strains in the central 10-90% of a gene are color coded by the insertion's strand.
Usually, "+" means that the selectable marker is encoded on the forward strand,
i.e., transcribed rightward.
500 nt GFF4224 and GFF4225 are separated by 14 nucleotides GFF4225 and GFF4226 overlap by 1 nucleotides GFF4226 and GFF4227 are separated by 33 nucleotides
GFF4224 - 3-isopropylmalate dehydratase small subunit (EC 4.2.1.33), at 4,408 to 5,034
GFF4224
GFF4225 - Fumarylacetoacetate hydrolase family protein, at 5,049 to 5,927
GFF4225
GFF4226 - Putative hydrolase or acyltransferase, at 5,927 to 6,841
GFF4226
GFF4227 - Putative LysR family transcriptional regulator, at 6,875 to 7,840
GFF4227
Position (kb) 5
6
7 Strain fitness (log2 ratio) -1
0
1
2 at 4.952 kb on - strand, within GFF4224 at 4.987 kb on + strand at 5.096 kb on + strand at 5.146 kb on - strand, within GFF4225 at 5.198 kb on - strand, within GFF4225 at 5.482 kb on + strand, within GFF4225 at 5.487 kb on + strand, within GFF4225 at 5.508 kb on + strand, within GFF4225 at 5.509 kb on - strand, within GFF4225 at 5.511 kb on - strand, within GFF4225 at 5.541 kb on - strand, within GFF4225 at 5.633 kb on - strand, within GFF4225 at 5.688 kb on - strand, within GFF4225 at 5.718 kb on + strand, within GFF4225 at 5.719 kb on - strand, within GFF4225 at 5.719 kb on - strand, within GFF4225 at 5.720 kb on + strand, within GFF4225 at 5.720 kb on + strand, within GFF4225 at 5.721 kb on - strand, within GFF4225 at 5.722 kb on + strand, within GFF4225 at 5.722 kb on + strand, within GFF4225 at 5.722 kb on + strand, within GFF4225 at 5.732 kb on + strand, within GFF4225 at 5.932 kb on + strand at 6.369 kb on - strand, within GFF4226 at 6.487 kb on + strand, within GFF4226 at 6.694 kb on - strand, within GFF4226 at 6.702 kb on + strand, within GFF4226 at 6.702 kb on + strand, within GFF4226 at 6.705 kb on - strand, within GFF4226 at 6.705 kb on - strand, within GFF4226 at 6.705 kb on - strand, within GFF4226 at 6.706 kb on + strand, within GFF4226 at 6.706 kb on + strand, within GFF4226 at 6.798 kb on - strand at 6.839 kb on + strand at 6.840 kb on - strand at 7.006 kb on - strand, within GFF4227 at 7.201 kb on + strand, within GFF4227 at 7.201 kb on + strand, within GFF4227 at 7.238 kb on + strand, within GFF4227 at 7.238 kb on + strand, within GFF4227 at 7.239 kb on - strand, within GFF4227 at 7.283 kb on - strand, within GFF4227 at 7.534 kb on - strand, within GFF4227 at 7.606 kb on + strand, within GFF4227 at 7.665 kb on - strand, within GFF4227 at 7.666 kb on + strand, within GFF4227 at 7.732 kb on - strand, within GFF4227 at 7.834 kb on - strand at 7.834 kb on - strand
Per-strain Table
Position Strand Gene LocusTag Fraction LB_plus_SM_buffer with EIP16_phage 0.875 MOI remove 4,952 - GFF4224 0.87 -0.9 4,987 + -0.0 5,096 + -0.7 5,146 - GFF4225 0.11 +0.1 5,198 - GFF4225 0.17 +0.5 5,482 + GFF4225 0.49 +0.3 5,487 + GFF4225 0.50 -0.5 5,508 + GFF4225 0.52 -0.4 5,509 - GFF4225 0.52 +0.7 5,511 - GFF4225 0.53 +0.5 5,541 - GFF4225 0.56 +0.2 5,633 - GFF4225 0.66 +0.4 5,688 - GFF4225 0.73 -0.2 5,718 + GFF4225 0.76 +0.1 5,719 - GFF4225 0.76 +0.2 5,719 - GFF4225 0.76 -0.1 5,720 + GFF4225 0.76 -0.8 5,720 + GFF4225 0.76 -0.2 5,721 - GFF4225 0.76 +0.6 5,722 + GFF4225 0.77 +1.2 5,722 + GFF4225 0.77 -0.1 5,722 + GFF4225 0.77 +2.0 5,732 + GFF4225 0.78 -0.1 5,932 + -0.4 6,369 - GFF4226 0.48 -0.1 6,487 + GFF4226 0.61 -0.1 6,694 - GFF4226 0.84 +0.1 6,702 + GFF4226 0.85 -0.7 6,702 + GFF4226 0.85 +0.2 6,705 - GFF4226 0.85 -0.6 6,705 - GFF4226 0.85 +0.5 6,705 - GFF4226 0.85 +0.7 6,706 + GFF4226 0.85 -0.2 6,706 + GFF4226 0.85 -1.0 6,798 - -0.5 6,839 + -0.1 6,840 - +0.5 7,006 - GFF4227 0.14 -0.5 7,201 + GFF4227 0.34 -0.1 7,201 + GFF4227 0.34 -0.3 7,238 + GFF4227 0.38 -0.2 7,238 + GFF4227 0.38 -0.3 7,239 - GFF4227 0.38 -1.3 7,283 - GFF4227 0.42 -0.1 7,534 - GFF4227 0.68 +1.5 7,606 + GFF4227 0.76 -0.7 7,665 - GFF4227 0.82 +0.1 7,666 + GFF4227 0.82 -0.1 7,732 - GFF4227 0.89 -0.4 7,834 - -0.3 7,834 - +0.1
Or see this region's nucleotide sequence