Experiment: LB_plus_SM_buffer with EIP16_phage 0.875 MOI
Only strains with sufficient reads to estimate fitness are shown,
but the strain fitness values are still rather noisy and may be biased towards zero.
Strains near the edge of a gene are not shown as being associated with that
gene (they are in grey).
Strains in the central 10-90% of a gene are color coded by the insertion's strand.
Usually, "+" means that the selectable marker is encoded on the forward strand,
i.e., transcribed rightward.
500 nt GFF3983 and GFF4034 are separated by 163 nucleotides GFF4034 and GFF3984 are separated by 408 nucleotides GFF3984 and GFF3985 are separated by 13 nucleotides GFF3985 and GFF4035 are separated by 167 nucleotides GFF4035 and GFF3986 are separated by 100 nucleotides
GFF3983 - FIG01045503: hypothetical protein, at 259,922 to 260,395
GFF3983
GFF4034 - tRNA-Asn-GTT, at 260,559 to 260,634
GFF4034
GFF3984 - FIG01048048: hypothetical protein, at 261,043 to 261,231
GFF3984
GFF3985 - Integrase, at 261,245 to 261,361
GFF3985
GFF4035 - tRNA-Asn-GTT, at 261,529 to 261,604
GFF4035
GFF3986 - FIG01220476: hypothetical protein, at 261,705 to 262,502
GFF3986
Position (kb) 261
262 Strain fitness (log2 ratio) -1
0
1 at 260.654 kb on + strand at 260.654 kb on + strand at 260.692 kb on - strand at 260.825 kb on - strand at 261.088 kb on + strand, within GFF3984 at 261.346 kb on + strand, within GFF3985 at 261.706 kb on + strand at 261.900 kb on + strand, within GFF3986 at 261.900 kb on + strand, within GFF3986 at 261.901 kb on - strand, within GFF3986 at 262.156 kb on - strand, within GFF3986 at 262.183 kb on - strand, within GFF3986 at 262.302 kb on - strand, within GFF3986
Per-strain Table
Position Strand Gene LocusTag Fraction LB_plus_SM_buffer with EIP16_phage 0.875 MOI remove 260,654 + +1.3 260,654 + +1.5 260,692 - -0.2 260,825 - +0.3 261,088 + GFF3984 0.24 -0.1 261,346 + GFF3985 0.86 -0.6 261,706 + -0.4 261,900 + GFF3986 0.24 +0.6 261,900 + GFF3986 0.24 +1.7 261,901 - GFF3986 0.25 +0.2 262,156 - GFF3986 0.57 +0.2 262,183 - GFF3986 0.60 +0.0 262,302 - GFF3986 0.75 -0.4
Or see this region's nucleotide sequence