Experiment: LB_plus_SM_buffer with EIP16_phage 0.875 MOI
Only strains with sufficient reads to estimate fitness are shown,
but the strain fitness values are still rather noisy and may be biased towards zero.
Strains near the edge of a gene are not shown as being associated with that
gene (they are in grey).
Strains in the central 10-90% of a gene are color coded by the insertion's strand.
Usually, "+" means that the selectable marker is encoded on the forward strand,
i.e., transcribed rightward.
500 nt GFF3908 and GFF3909 overlap by 1 nucleotides GFF3909 and GFF3910 overlap by 1 nucleotides GFF3910 and GFF3911 overlap by 4 nucleotides
GFF3908 - Phosphoribosylformimino-5-aminoimidazole carboxamide ribotide isomerase (EC 5.3.1.16), at 189,465 to 190,202
GFF3908
GFF3909 - Imidazole glycerol phosphate synthase amidotransferase subunit (EC 2.4.2.-), at 190,202 to 190,792
GFF3909
GFF3910 - Histidinol-phosphatase (EC 3.1.3.15) / Imidazoleglycerol-phosphate dehydratase (EC 4.2.1.19), at 190,792 to 191,859
GFF3910
GFF3911 - Histidinol-phosphate aminotransferase (EC 2.6.1.9), at 191,856 to 192,935
GFF3911
Position (kb) 190
191
192 Strain fitness (log2 ratio) -1
0
1 at 189.808 kb on - strand, within GFF3908 at 190.186 kb on - strand at 190.203 kb on + strand at 190.268 kb on + strand, within GFF3909 at 190.269 kb on - strand, within GFF3909 at 190.586 kb on - strand, within GFF3909 at 190.593 kb on + strand, within GFF3909 at 190.595 kb on - strand, within GFF3909 at 190.754 kb on + strand at 191.005 kb on - strand, within GFF3910 at 191.016 kb on + strand, within GFF3910 at 191.164 kb on + strand, within GFF3910 at 191.187 kb on - strand, within GFF3910 at 191.187 kb on - strand, within GFF3910 at 191.187 kb on - strand, within GFF3910 at 191.697 kb on + strand, within GFF3910 at 191.938 kb on - strand at 192.029 kb on + strand, within GFF3911 at 192.029 kb on + strand, within GFF3911 at 192.216 kb on + strand, within GFF3911 at 192.367 kb on + strand, within GFF3911 at 192.368 kb on - strand, within GFF3911 at 192.368 kb on - strand, within GFF3911 at 192.441 kb on - strand, within GFF3911 at 192.441 kb on - strand, within GFF3911 at 192.441 kb on - strand, within GFF3911 at 192.467 kb on + strand, within GFF3911 at 192.562 kb on - strand, within GFF3911 at 192.590 kb on - strand, within GFF3911 at 192.770 kb on + strand, within GFF3911
Per-strain Table
Position Strand Gene LocusTag Fraction LB_plus_SM_buffer with EIP16_phage 0.875 MOI remove 189,808 - GFF3908 0.46 -0.4 190,186 - -0.1 190,203 + +0.3 190,268 + GFF3909 0.11 +0.5 190,269 - GFF3909 0.11 -1.1 190,586 - GFF3909 0.65 -0.3 190,593 + GFF3909 0.66 -0.4 190,595 - GFF3909 0.66 +0.3 190,754 + +0.9 191,005 - GFF3910 0.20 -0.1 191,016 + GFF3910 0.21 +1.3 191,164 + GFF3910 0.35 +0.9 191,187 - GFF3910 0.37 +0.2 191,187 - GFF3910 0.37 -0.7 191,187 - GFF3910 0.37 +0.8 191,697 + GFF3910 0.85 -0.4 191,938 - -0.6 192,029 + GFF3911 0.16 +0.7 192,029 + GFF3911 0.16 +0.7 192,216 + GFF3911 0.33 -0.7 192,367 + GFF3911 0.47 -0.4 192,368 - GFF3911 0.47 -0.4 192,368 - GFF3911 0.47 -0.1 192,441 - GFF3911 0.54 -0.2 192,441 - GFF3911 0.54 +0.1 192,441 - GFF3911 0.54 -0.3 192,467 + GFF3911 0.57 +0.1 192,562 - GFF3911 0.65 -0.5 192,590 - GFF3911 0.68 -0.3 192,770 + GFF3911 0.85 +0.2
Or see this region's nucleotide sequence