Experiment: LB_plus_SM_buffer with EIP16_phage 0.875 MOI
Only strains with sufficient reads to estimate fitness are shown,
but the strain fitness values are still rather noisy and may be biased towards zero.
Strains near the edge of a gene are not shown as being associated with that
gene (they are in grey).
Strains in the central 10-90% of a gene are color coded by the insertion's strand.
Usually, "+" means that the selectable marker is encoded on the forward strand,
i.e., transcribed rightward.
500 nt GFF3886 and GFF3887 are separated by 376 nucleotides GFF3887 and GFF3888 overlap by 1 nucleotides GFF3888 and GFF3889 are separated by 41 nucleotides
GFF3886 - UTP--glucose-1-phosphate uridylyltransferase (EC 2.7.7.9), at 165,003 to 165,896
GFF3886
GFF3887 - dTDP-glucose 4,6-dehydratase (EC 4.2.1.46), at 166,273 to 167,358
GFF3887
GFF3888 - dTDP-4-dehydrorhamnose reductase (EC 1.1.1.133), at 167,358 to 168,257
GFF3888
GFF3889 - Glucose-1-phosphate thymidylyltransferase (EC 2.7.7.24), at 168,299 to 169,183
GFF3889
Position (kb) 166
167
168 Strain fitness (log2 ratio) -6
-5
-4
-3
-2
-1
0
1
2 at 165.275 kb on + strand, within GFF3886 at 165.341 kb on + strand, within GFF3886 at 165.341 kb on + strand, within GFF3886 at 165.342 kb on - strand, within GFF3886 at 165.435 kb on + strand, within GFF3886 at 165.435 kb on + strand, within GFF3886 at 165.436 kb on - strand, within GFF3886 at 165.437 kb on + strand, within GFF3886 at 165.783 kb on + strand, within GFF3886 at 165.784 kb on - strand, within GFF3886 at 165.874 kb on + strand at 165.875 kb on - strand at 166.085 kb on + strand at 166.158 kb on - strand at 166.585 kb on + strand, within GFF3887 at 166.612 kb on + strand, within GFF3887 at 166.764 kb on + strand, within GFF3887 at 166.825 kb on + strand, within GFF3887 at 166.857 kb on + strand, within GFF3887 at 166.860 kb on + strand, within GFF3887 at 166.864 kb on + strand, within GFF3887 at 166.864 kb on + strand, within GFF3887 at 166.973 kb on + strand, within GFF3887 at 166.975 kb on + strand, within GFF3887 at 166.976 kb on - strand, within GFF3887 at 166.976 kb on - strand, within GFF3887 at 167.004 kb on + strand, within GFF3887 at 167.082 kb on - strand, within GFF3887 at 167.086 kb on - strand, within GFF3887 at 167.149 kb on + strand, within GFF3887 at 167.150 kb on - strand, within GFF3887 at 167.150 kb on - strand, within GFF3887 at 167.341 kb on + strand at 167.368 kb on + strand at 167.510 kb on + strand, within GFF3888 at 167.557 kb on + strand, within GFF3888 at 167.673 kb on + strand, within GFF3888 at 167.828 kb on + strand, within GFF3888 at 167.828 kb on + strand, within GFF3888 at 168.043 kb on + strand, within GFF3888 at 168.095 kb on + strand, within GFF3888 at 168.264 kb on + strand
Per-strain Table
Position Strand Gene LocusTag Fraction LB_plus_SM_buffer with EIP16_phage 0.875 MOI remove 165,275 + GFF3886 0.30 -3.1 165,341 + GFF3886 0.38 -3.3 165,341 + GFF3886 0.38 -3.9 165,342 - GFF3886 0.38 -3.3 165,435 + GFF3886 0.48 -0.3 165,435 + GFF3886 0.48 -1.0 165,436 - GFF3886 0.48 -4.4 165,437 + GFF3886 0.49 -1.4 165,783 + GFF3886 0.87 -1.4 165,784 - GFF3886 0.87 -0.7 165,874 + -1.4 165,875 - -0.7 166,085 + -4.5 166,158 - -1.9 166,585 + GFF3887 0.29 -3.6 166,612 + GFF3887 0.31 -5.8 166,764 + GFF3887 0.45 -5.4 166,825 + GFF3887 0.51 -3.5 166,857 + GFF3887 0.54 -4.6 166,860 + GFF3887 0.54 -4.1 166,864 + GFF3887 0.54 -4.8 166,864 + GFF3887 0.54 -4.5 166,973 + GFF3887 0.64 -2.9 166,975 + GFF3887 0.65 -2.3 166,976 - GFF3887 0.65 -0.7 166,976 - GFF3887 0.65 -2.1 167,004 + GFF3887 0.67 -2.7 167,082 - GFF3887 0.74 +0.1 167,086 - GFF3887 0.75 -1.4 167,149 + GFF3887 0.81 -3.2 167,150 - GFF3887 0.81 +1.9 167,150 - GFF3887 0.81 -0.1 167,341 + -4.0 167,368 + +0.6 167,510 + GFF3888 0.17 -0.4 167,557 + GFF3888 0.22 +0.4 167,673 + GFF3888 0.35 -0.8 167,828 + GFF3888 0.52 +0.2 167,828 + GFF3888 0.52 +0.2 168,043 + GFF3888 0.76 +0.4 168,095 + GFF3888 0.82 +1.6 168,264 + +0.9
Or see this region's nucleotide sequence