Experiment: LB_plus_SM_buffer with EIP16_phage 0.875 MOI
Only strains with sufficient reads to estimate fitness are shown,
but the strain fitness values are still rather noisy and may be biased towards zero.
Strains near the edge of a gene are not shown as being associated with that
gene (they are in grey).
Strains in the central 10-90% of a gene are color coded by the insertion's strand.
Usually, "+" means that the selectable marker is encoded on the forward strand,
i.e., transcribed rightward.
500 nt GFF3792 and GFF3793 are separated by 204 nucleotides GFF3793 and GFF3794 are separated by 2 nucleotides GFF3794 and GFF3795 are separated by 176 nucleotides
GFF3792 - Pyridine nucleotide-disulphide oxidoreductase family protein, at 62,218 to 63,459
GFF3792
GFF3793 - FIG00638799: hypothetical protein, at 63,664 to 63,903
GFF3793
GFF3794 - SanA protein, at 63,906 to 64,625
GFF3794
GFF3795 - Cytidine deaminase (EC 3.5.4.5), at 64,802 to 65,686
GFF3795
Position (kb) 63
64
65 Strain fitness (log2 ratio) -3
-2
-1
0
1 at 63.022 kb on + strand, within GFF3792 at 63.199 kb on - strand, within GFF3792 at 63.895 kb on + strand at 64.161 kb on - strand, within GFF3794 at 64.202 kb on + strand, within GFF3794 at 64.339 kb on + strand, within GFF3794 at 64.386 kb on - strand, within GFF3794 at 64.430 kb on - strand, within GFF3794 at 64.459 kb on + strand, within GFF3794 at 64.460 kb on - strand, within GFF3794 at 64.510 kb on - strand, within GFF3794 at 64.520 kb on - strand, within GFF3794 at 64.523 kb on + strand, within GFF3794 at 64.524 kb on - strand, within GFF3794 at 64.526 kb on - strand, within GFF3794 at 64.681 kb on + strand at 64.681 kb on + strand at 64.681 kb on + strand at 64.682 kb on - strand at 65.069 kb on - strand, within GFF3795 at 65.078 kb on - strand, within GFF3795 at 65.078 kb on - strand, within GFF3795 at 65.086 kb on + strand, within GFF3795 at 65.135 kb on - strand, within GFF3795 at 65.221 kb on + strand, within GFF3795 at 65.222 kb on - strand, within GFF3795 at 65.516 kb on + strand, within GFF3795
Per-strain Table
Position Strand Gene LocusTag Fraction LB_plus_SM_buffer with EIP16_phage 0.875 MOI remove 63,022 + GFF3792 0.65 +0.3 63,199 - GFF3792 0.79 +0.3 63,895 + -0.0 64,161 - GFF3794 0.35 -2.0 64,202 + GFF3794 0.41 +1.4 64,339 + GFF3794 0.60 -2.2 64,386 - GFF3794 0.67 +0.6 64,430 - GFF3794 0.73 -0.5 64,459 + GFF3794 0.77 -0.8 64,460 - GFF3794 0.77 -0.3 64,510 - GFF3794 0.84 -1.8 64,520 - GFF3794 0.85 -2.1 64,523 + GFF3794 0.86 -0.6 64,524 - GFF3794 0.86 -2.1 64,526 - GFF3794 0.86 -3.3 64,681 + -1.6 64,681 + -1.3 64,681 + -0.5 64,682 - -0.7 65,069 - GFF3795 0.30 +0.4 65,078 - GFF3795 0.31 -0.1 65,078 - GFF3795 0.31 +0.0 65,086 + GFF3795 0.32 +0.5 65,135 - GFF3795 0.38 -0.7 65,221 + GFF3795 0.47 -0.4 65,222 - GFF3795 0.47 -0.3 65,516 + GFF3795 0.81 +0.5
Or see this region's nucleotide sequence